ilr3 - nrt /NIA1/SWEET12模块调控苹果氮素吸收和利用。

IF 8.1 Q1 HORTICULTURE
Hong-Liang Li, Ran-Xin Liu, Xiang Wu, Xin-Long Guo, Shan-Shan Li, Tian-Tian Wang, Yan-Yan Guo, Xiao-Fei Wang, Chun-Xiang You
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引用次数: 0

摘要

氮(N)是植物生理代谢、生长发育所必需的。植物为了适应环境氮水平的波动,进化出了一个复杂的调控网络,可以有效地调控氮素的吸收和利用。然而,苹果对氮的吸收和利用调控机制尚不清楚。在这里,我们通过酵母单杂交(Y1H)筛选发现MdILR3 (IAA-LEUCINE RESISTANT3)是MdNRT2.4的上游调节因子。MdILR3过表达显著上调MdNRT2.3/2.4和MdNIA1的表达,导致硝酸盐含量和硝酸盐还原酶活性增加。Y1H和EMSA分析显示,MdILR3直接与MdNRT2.3/2.4和MdNIA1的启动子相互作用。此外,MdILR3可以直接结合MdSWEET12的启动子并激活其表达,从而调节蔗糖运输,为根系吸收N提供能量。综上所述,我们提供的生理和分子证据表明,MdILR3可能通过激活与氮吸收和糖转运相关的基因表达来积极调节硝酸盐反应。结果表明,对苹果进行遗传改良可以提高其对氮素的吸收和利用能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The ILR3-NRTs/NIA1/SWEET12 module regulates nitrogen uptake and utilization in apple.

Nitrogen (N) is essential for the physiological metabolism, growth, and development of plants. Plants have evolved a complex regulatory network for the efficient regulation of N uptake and utilization to adapt to fluctuations in environmental N levels. However, the mechanisms underlying the regulation of N absorption and utilization in apple remain unclear. Here, we identified MdILR3 (IAA-LEUCINE RESISTANT3) as an upstream regulator of MdNRT2.4 through yeast one-hybrid (Y1H) screening. MdILR3 overexpression significantly up-regulated the expression of MdNRT2.3/2.4 and MdNIA1, resulting in an increase in nitrate content and nitrate reductase activity. Y1H and EMSA assays revealed that MdILR3 directly interacted with the promoters of MdNRT2.3/2.4 and MdNIA1. Furthermore, MdILR3 can directly bind to the promoter of MdSWEET12 and activate its expression, thereby regulating sucrose transport to provide energy for N uptake in roots. In summary, we provide physiological and molecular evidence suggesting that MdILR3 may positively regulate nitrate response by activating the expression of genes related to N uptake and sugar transport. Our findings suggest that genetic improvements in apple could enhance its ability to absorb and utilize N.

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来源期刊
Molecular Horticulture
Molecular Horticulture horticultural research-
CiteScore
8.00
自引率
0.00%
发文量
24
审稿时长
12 weeks
期刊介绍: Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.
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