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引用次数: 0
摘要
DNA甲基化是一种重要的表观遗传修饰，在植物有丝分裂和减数分裂细胞分裂中稳定地遗传。它受到多种表观遗传途径的调节，甲基化的改变可以导致独立于DNA序列变化的表型变异。在这项研究中，发现染色质重塑器DDM1引发的DNA甲基化变化（DNA甲基化1的减少）影响拟南芥叶片表型。ddm1突变显著降低了全基因组DNA甲基化，特别是转座元件（te）。TEs的低甲基化激活了下游基因，包括DPA4 (THE APEX中发育相关的PCG靶4)，它抑制了CUC2（杯形子叶2），这是叶片分形的关键调节因子。重要的是，DPA4启动子的甲基化变化在F2代中稳定遗传。本研究发现DPA4是受ddm1突变影响的基因，该突变显著影响拟南芥叶片的锯齿形。这些发现突出了诱导表观遗传修饰对植物发育的影响，为进一步探索可遗传的表观遗传性状提供了基础。
DDM1-Mediated DNA Methylation Regulates Leaf Serration in Arabidopsis thaliana.
DNA methylation is a crucial epigenetic modification that is stably inherited across both mitotic and meiotic cell divisions in plants. It is regulated by multiple epigenetic pathways, and alterations in methylation can lead to phenotypic variation independent of changes in the DNA sequence. In this study, changes in DNA methylation triggered by the chromatin remodeler DDM1 (DECREASE IN DNA METHYLATION 1) were found to influence leaf phenotypes in Arabidopsis thaliana. The ddm1 mutation dramatically reduced genome-wide DNA methylation, particularly in transposable elements (TEs). Hypomethylation of TEs activated downstream genes, including DPA4 (DEVELOPMENT-RELATED PCG TARGET IN THE APEX 4), which suppresses CUC2 (CUP-SHAPED COTYLEDON 2), a key regulator of leaf serration. Importantly, methylation changes in the DPA4 promoter were stably inherited in the F2 generation. This study identified DPA4 as a gene affected by the ddm1 mutation, which significantly affects leaf serration in A. thaliana. These findings highlight the impact of induced epigenetic modifications on plant development, providing a basis for further exploration of heritable epigenetic traits.
期刊介绍：
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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