Mennat-Elrahman A Fahmy, Amany A Abdel-Aal, Soad I Hassan, Maisa A Shalaby, Marwa Esmat
{"title":"1型糖尿病小鼠模型与地塞米松免疫功能低下小鼠模型隐孢子虫病伴回肠隐窝超微结构变化","authors":"Mennat-Elrahman A Fahmy, Amany A Abdel-Aal, Soad I Hassan, Maisa A Shalaby, Marwa Esmat","doi":"10.1007/s12639-025-01789-0","DOIUrl":null,"url":null,"abstract":"<p><p>Cryptosporidiosis is an enteric infection caused by <i>Cryptosporidium spp.</i> The severity of the disease depends mainly on the immune status of the host. The infection is self-limited in immunocompetent individuals but in immunocompromised patients, it can be severe and threatening. To provide new insights into a better understanding of the pathogenesis of the infection and the impact of immune modulation on the course of the disease, we used 4 groups of Swiss-Albino mice; dexamethasone (DEX) group, the diabetic group, the DEX-infected group, and the diabetic-infected group. The blood glucose levels, oocyst shedding, mortality rates, and ultrastructural changes among study groups were observed and documented. The diabetic groups showed hyperglycemia while the DEX-infected group showed significantly higher oocyst shedding rates compared to the diabetic-infected group (<i>P</i> > 0.005). At the end of the experiment, the DEX groups showed higher mortality rates. Regarding the ultrastructural ileal crypt changes recorded in all groups, the DEX-infected group showed the severest changes with significantly lower numbers of Paneth cells, depletion of Paneth cell granules, and increased number of apoptotic crypt bodies significantly (<i>P</i> > 0.005) compared to the diabetic-infected group. On the contrary, the diabetic-infected group showed a significant expansion of Paneth cells with an increased number of granules and a significantly decreased number of apoptotic crypt bodies (<i>P</i> > 0.005). However, both models failed to control the infection properly highlighting the importance of early diagnosis and treatment of suspected immunocompromised cases.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 3","pages":"712-724"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399472/pdf/","citationCount":"0","resultStr":"{\"title\":\"The ileal crypt ultrastructural changes accompanying cryptosporidiosis in type 1 diabetic mouse model versus dexamethasone-immunocompromised mouse model.\",\"authors\":\"Mennat-Elrahman A Fahmy, Amany A Abdel-Aal, Soad I Hassan, Maisa A Shalaby, Marwa Esmat\",\"doi\":\"10.1007/s12639-025-01789-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cryptosporidiosis is an enteric infection caused by <i>Cryptosporidium spp.</i> The severity of the disease depends mainly on the immune status of the host. The infection is self-limited in immunocompetent individuals but in immunocompromised patients, it can be severe and threatening. To provide new insights into a better understanding of the pathogenesis of the infection and the impact of immune modulation on the course of the disease, we used 4 groups of Swiss-Albino mice; dexamethasone (DEX) group, the diabetic group, the DEX-infected group, and the diabetic-infected group. The blood glucose levels, oocyst shedding, mortality rates, and ultrastructural changes among study groups were observed and documented. The diabetic groups showed hyperglycemia while the DEX-infected group showed significantly higher oocyst shedding rates compared to the diabetic-infected group (<i>P</i> > 0.005). At the end of the experiment, the DEX groups showed higher mortality rates. Regarding the ultrastructural ileal crypt changes recorded in all groups, the DEX-infected group showed the severest changes with significantly lower numbers of Paneth cells, depletion of Paneth cell granules, and increased number of apoptotic crypt bodies significantly (<i>P</i> > 0.005) compared to the diabetic-infected group. On the contrary, the diabetic-infected group showed a significant expansion of Paneth cells with an increased number of granules and a significantly decreased number of apoptotic crypt bodies (<i>P</i> > 0.005). However, both models failed to control the infection properly highlighting the importance of early diagnosis and treatment of suspected immunocompromised cases.</p>\",\"PeriodicalId\":16664,\"journal\":{\"name\":\"Journal of Parasitic Diseases\",\"volume\":\"49 3\",\"pages\":\"712-724\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399472/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Parasitic Diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s12639-025-01789-0\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/2/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Immunology and Microbiology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Parasitic Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12639-025-01789-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/25 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
The ileal crypt ultrastructural changes accompanying cryptosporidiosis in type 1 diabetic mouse model versus dexamethasone-immunocompromised mouse model.
Cryptosporidiosis is an enteric infection caused by Cryptosporidium spp. The severity of the disease depends mainly on the immune status of the host. The infection is self-limited in immunocompetent individuals but in immunocompromised patients, it can be severe and threatening. To provide new insights into a better understanding of the pathogenesis of the infection and the impact of immune modulation on the course of the disease, we used 4 groups of Swiss-Albino mice; dexamethasone (DEX) group, the diabetic group, the DEX-infected group, and the diabetic-infected group. The blood glucose levels, oocyst shedding, mortality rates, and ultrastructural changes among study groups were observed and documented. The diabetic groups showed hyperglycemia while the DEX-infected group showed significantly higher oocyst shedding rates compared to the diabetic-infected group (P > 0.005). At the end of the experiment, the DEX groups showed higher mortality rates. Regarding the ultrastructural ileal crypt changes recorded in all groups, the DEX-infected group showed the severest changes with significantly lower numbers of Paneth cells, depletion of Paneth cell granules, and increased number of apoptotic crypt bodies significantly (P > 0.005) compared to the diabetic-infected group. On the contrary, the diabetic-infected group showed a significant expansion of Paneth cells with an increased number of granules and a significantly decreased number of apoptotic crypt bodies (P > 0.005). However, both models failed to control the infection properly highlighting the importance of early diagnosis and treatment of suspected immunocompromised cases.
期刊介绍:
The primary constituency of the Journal of Parasitic Diseases is parasitology. It publishes original research papers (pure, applied and clinical), which contribute significantly to any area of parasitology. Research papers on various aspects of cellular and molecular parasitology are welcome.