{"title":"纳米硒对热应激下虹鳟鱼脾脏组织病理变化及基因表达的影响。","authors":"Junhao Lu, Zhe Liu, Jiahui Zhang, Lanlan Li, Guiyan Zhao, Yucai Pan, Jinqiang Quan, Guolin Song","doi":"10.1007/s10695-025-01576-x","DOIUrl":null,"url":null,"abstract":"<p><p>Rainbow trout(Oncorhynchus mykiss) is a typical cold-water fish often threatened by high summer temperatures. Nano-selenium as a feed additive can improve the antioxidant capacity of the body and relieve stress. In this study, different levels of nano-selenium (0, 5 and 10 mg/kg) were added to the feed of rainbow trout to determine the changes in spleen structure and expression of related genes in rainbow trout at the proper temperature (18℃) and heat stress temperature (24℃). The results showed that the addition of 5 mg/kg nano-selenium had a more obvious protective effect on rainbow trout spleen. The addition of nano-selenium improved the antioxidant capacity and immunity of rainbow trout spleen at 18 °C. After heat stress, the addition of 5 mg/kg of nano-selenium significantly increased the expression levels of spleen heat shock protein genes, such as HSP10, HSP47, HSP70, HSP90, antioxidant-related genes, such as glutathione peroxidase (GPx), thioredoxin reductase (Trx), superoxide dismutase (SOD1), catalase (CAT) and immune-related genes, such as tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Immunoglobulin M (IgM), Complement Component 3 (C3), resulting in the improvement of antioxidant capacity and immunity, and the protection of the spleen to alleviate the damages caused by heat stress. This study provides a theoretical basis for the development of anti-heat stress feeds for rainbow trout.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"158"},"PeriodicalIF":2.5000,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of nano-selenium on the histopathological changes and gene expression of rainbow trout (Oncorhynchus mykiss) spleen under heat stress.\",\"authors\":\"Junhao Lu, Zhe Liu, Jiahui Zhang, Lanlan Li, Guiyan Zhao, Yucai Pan, Jinqiang Quan, Guolin Song\",\"doi\":\"10.1007/s10695-025-01576-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Rainbow trout(Oncorhynchus mykiss) is a typical cold-water fish often threatened by high summer temperatures. Nano-selenium as a feed additive can improve the antioxidant capacity of the body and relieve stress. In this study, different levels of nano-selenium (0, 5 and 10 mg/kg) were added to the feed of rainbow trout to determine the changes in spleen structure and expression of related genes in rainbow trout at the proper temperature (18℃) and heat stress temperature (24℃). The results showed that the addition of 5 mg/kg nano-selenium had a more obvious protective effect on rainbow trout spleen. The addition of nano-selenium improved the antioxidant capacity and immunity of rainbow trout spleen at 18 °C. After heat stress, the addition of 5 mg/kg of nano-selenium significantly increased the expression levels of spleen heat shock protein genes, such as HSP10, HSP47, HSP70, HSP90, antioxidant-related genes, such as glutathione peroxidase (GPx), thioredoxin reductase (Trx), superoxide dismutase (SOD1), catalase (CAT) and immune-related genes, such as tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Immunoglobulin M (IgM), Complement Component 3 (C3), resulting in the improvement of antioxidant capacity and immunity, and the protection of the spleen to alleviate the damages caused by heat stress. This study provides a theoretical basis for the development of anti-heat stress feeds for rainbow trout.</p>\",\"PeriodicalId\":12274,\"journal\":{\"name\":\"Fish Physiology and Biochemistry\",\"volume\":\"51 5\",\"pages\":\"158\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fish Physiology and Biochemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10695-025-01576-x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish Physiology and Biochemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10695-025-01576-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effects of nano-selenium on the histopathological changes and gene expression of rainbow trout (Oncorhynchus mykiss) spleen under heat stress.
Rainbow trout(Oncorhynchus mykiss) is a typical cold-water fish often threatened by high summer temperatures. Nano-selenium as a feed additive can improve the antioxidant capacity of the body and relieve stress. In this study, different levels of nano-selenium (0, 5 and 10 mg/kg) were added to the feed of rainbow trout to determine the changes in spleen structure and expression of related genes in rainbow trout at the proper temperature (18℃) and heat stress temperature (24℃). The results showed that the addition of 5 mg/kg nano-selenium had a more obvious protective effect on rainbow trout spleen. The addition of nano-selenium improved the antioxidant capacity and immunity of rainbow trout spleen at 18 °C. After heat stress, the addition of 5 mg/kg of nano-selenium significantly increased the expression levels of spleen heat shock protein genes, such as HSP10, HSP47, HSP70, HSP90, antioxidant-related genes, such as glutathione peroxidase (GPx), thioredoxin reductase (Trx), superoxide dismutase (SOD1), catalase (CAT) and immune-related genes, such as tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Immunoglobulin M (IgM), Complement Component 3 (C3), resulting in the improvement of antioxidant capacity and immunity, and the protection of the spleen to alleviate the damages caused by heat stress. This study provides a theoretical basis for the development of anti-heat stress feeds for rainbow trout.
期刊介绍:
Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.