Nathaniel Noblett , Tony Roenspies , Stephane Flibotte , Antonio Colavita
{"title":"核激素受体调节PAL-1/尾侧介导线虫腹侧神经束组装","authors":"Nathaniel Noblett , Tony Roenspies , Stephane Flibotte , Antonio Colavita","doi":"10.1016/j.ydbio.2025.08.023","DOIUrl":null,"url":null,"abstract":"<div><div>The regulatory network governed by CDX/<em>Caudal</em> family transcription factors plays critical roles in shaping embryonic neural development. In <em>C. elegans</em>, we found that proper expression of <em>pal-1</em>, the <em>C. elegans Caudal</em> homologue, is required for correct positioning of motor neuron cell bodies in the first larval stage ventral nerve cord (VNC). We identified an upstream regulatory region within the <em>pal-1</em> promoter that drives <em>pal-1</em> expression in a subset of DD and DA neuronal progenitors. We also show that SEX-1, a nuclear hormone receptor, is required for motor neuron positioning in the VNC. Loss of <em>sex-1</em> results in neuronal positioning defects similar to those observed in <em>pal-1</em> mutants. This is in part due to a requirement for SEX-1 in promoting <em>pal-1</em> expression in DD and DA neuronal progenitors during VNC assembly. Double mutant analysis further suggests that <em>sex-1</em> also has <em>pal-1</em>-independent functions. Together, these findings define a transcriptional hierarchy in which the SEX-1 nuclear hormone receptor regulates the tissue-specific activity of PAL-1 to promote proper motor neuron positioning in the VNC and highlight a conserved role for NHR and CDX/Caudal family proteins in central nerve cord formation.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"528 ","pages":"Pages 13-26"},"PeriodicalIF":2.1000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nuclear hormone receptor regulation of PAL-1/Caudal mediates ventral nerve cord assembly in C. elegans\",\"authors\":\"Nathaniel Noblett , Tony Roenspies , Stephane Flibotte , Antonio Colavita\",\"doi\":\"10.1016/j.ydbio.2025.08.023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The regulatory network governed by CDX/<em>Caudal</em> family transcription factors plays critical roles in shaping embryonic neural development. In <em>C. elegans</em>, we found that proper expression of <em>pal-1</em>, the <em>C. elegans Caudal</em> homologue, is required for correct positioning of motor neuron cell bodies in the first larval stage ventral nerve cord (VNC). We identified an upstream regulatory region within the <em>pal-1</em> promoter that drives <em>pal-1</em> expression in a subset of DD and DA neuronal progenitors. We also show that SEX-1, a nuclear hormone receptor, is required for motor neuron positioning in the VNC. Loss of <em>sex-1</em> results in neuronal positioning defects similar to those observed in <em>pal-1</em> mutants. This is in part due to a requirement for SEX-1 in promoting <em>pal-1</em> expression in DD and DA neuronal progenitors during VNC assembly. Double mutant analysis further suggests that <em>sex-1</em> also has <em>pal-1</em>-independent functions. Together, these findings define a transcriptional hierarchy in which the SEX-1 nuclear hormone receptor regulates the tissue-specific activity of PAL-1 to promote proper motor neuron positioning in the VNC and highlight a conserved role for NHR and CDX/Caudal family proteins in central nerve cord formation.</div></div>\",\"PeriodicalId\":11070,\"journal\":{\"name\":\"Developmental biology\",\"volume\":\"528 \",\"pages\":\"Pages 13-26\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0012160625002404\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0012160625002404","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Nuclear hormone receptor regulation of PAL-1/Caudal mediates ventral nerve cord assembly in C. elegans
The regulatory network governed by CDX/Caudal family transcription factors plays critical roles in shaping embryonic neural development. In C. elegans, we found that proper expression of pal-1, the C. elegans Caudal homologue, is required for correct positioning of motor neuron cell bodies in the first larval stage ventral nerve cord (VNC). We identified an upstream regulatory region within the pal-1 promoter that drives pal-1 expression in a subset of DD and DA neuronal progenitors. We also show that SEX-1, a nuclear hormone receptor, is required for motor neuron positioning in the VNC. Loss of sex-1 results in neuronal positioning defects similar to those observed in pal-1 mutants. This is in part due to a requirement for SEX-1 in promoting pal-1 expression in DD and DA neuronal progenitors during VNC assembly. Double mutant analysis further suggests that sex-1 also has pal-1-independent functions. Together, these findings define a transcriptional hierarchy in which the SEX-1 nuclear hormone receptor regulates the tissue-specific activity of PAL-1 to promote proper motor neuron positioning in the VNC and highlight a conserved role for NHR and CDX/Caudal family proteins in central nerve cord formation.
期刊介绍:
Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.