核激素受体调节PAL-1/尾侧介导线虫腹侧神经束组装

IF 2.1 3区 生物学 Q2 DEVELOPMENTAL BIOLOGY
Nathaniel Noblett , Tony Roenspies , Stephane Flibotte , Antonio Colavita
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引用次数: 0

摘要

CDX/尾侧家族转录因子调控的调控网络在胚胎神经发育中起着关键作用。在线虫中,我们发现线虫尾侧同源基因pal-1的正确表达是线虫第一幼虫期腹侧神经索(VNC)运动神经元胞体正确定位所必需的。我们在pal-1启动子中发现了一个上游调控区域,该区域在DD和DA神经元祖细胞的一个子集中驱动pal-1表达。我们还表明,核激素受体SEX-1是运动神经元在VNC中的定位所必需的。性别-1缺失导致神经元定位缺陷,这与pal-1突变体中观察到的相似。这部分是由于在VNC组装期间,在DD和DA神经元祖细胞中促进pal-1表达需要SEX-1。双突变分析进一步表明,性别-1也具有与pal-1无关的功能。总之,这些发现定义了一个转录层次,其中性别-1核激素受体调节PAL-1的组织特异性活性,以促进VNC中适当的运动神经元定位,并强调了NHR和CDX/尾侧家族蛋白在中枢神经索形成中的保守作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Nuclear hormone receptor regulation of PAL-1/Caudal mediates ventral nerve cord assembly in C. elegans

Nuclear hormone receptor regulation of PAL-1/Caudal mediates ventral nerve cord assembly in C. elegans
The regulatory network governed by CDX/Caudal family transcription factors plays critical roles in shaping embryonic neural development. In C. elegans, we found that proper expression of pal-1, the C. elegans Caudal homologue, is required for correct positioning of motor neuron cell bodies in the first larval stage ventral nerve cord (VNC). We identified an upstream regulatory region within the pal-1 promoter that drives pal-1 expression in a subset of DD and DA neuronal progenitors. We also show that SEX-1, a nuclear hormone receptor, is required for motor neuron positioning in the VNC. Loss of sex-1 results in neuronal positioning defects similar to those observed in pal-1 mutants. This is in part due to a requirement for SEX-1 in promoting pal-1 expression in DD and DA neuronal progenitors during VNC assembly. Double mutant analysis further suggests that sex-1 also has pal-1-independent functions. Together, these findings define a transcriptional hierarchy in which the SEX-1 nuclear hormone receptor regulates the tissue-specific activity of PAL-1 to promote proper motor neuron positioning in the VNC and highlight a conserved role for NHR and CDX/Caudal family proteins in central nerve cord formation.
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来源期刊
Developmental biology
Developmental biology 生物-发育生物学
CiteScore
5.30
自引率
3.70%
发文量
182
审稿时长
1.5 months
期刊介绍: Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.
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