诱导多能干细胞系JHUi008-A的生成，来自健康女性供体

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-08-24 DOI:10.1016/j.scr.2025.103819

Christianne J. Chua , Deborah DiSilvestre , Rosy Joshi-Mukherjee , Leslie Tung , Gordon F. Tomaselli , Kenneth R. Boheler

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引用次数: 0

摘要

使用具有良好特征的人类诱导多能干细胞（hiPSCs）对于发育研究和疾病建模至关重要。在这里，我们报告了使用仙台病毒技术对来自健康女性供体的外周血单个核细胞（PBMCs）进行重编程后产生正常的雌性hiPSCs系。该细胞系已广泛用于中胚层来源心肌细胞的体外研究，并已在人类多能干细胞登记处（hPSCreg）中注册。它表现出正常的核型，表达干性标记，并表现出强大的三龄分化潜力。

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Generation of an induced pluripotent stem cell line, JHUi008-A, from a healthy female donor

The use of well characterized human induced pluripotent stem cells (hiPSCs) is essential for developmental studies and disease modeling. Here, we report the generation of a normal, female line of hiPSCs following reprogramming of peripheral blood mononuclear cells (PBMCs) derived from a healthy female donor using Sendai virus technology. This line, which has been extensively employed for the in vitro study of mesoderm-derived cardiomyocytes, is available and registered in the Human Pluripotent Stem Cell Registry (hPSCreg). It exhibits a normal karyotype, expresses stemness markers, and demonstrates robust trilineage differentiation potential.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.