登革病毒在沙特阿拉伯反复传入和隐传的分子进化见解

IF 11.9 1区医学 Q1 INFECTIOUS DISEASES

Journal of Infection Pub Date : 2025-09-01 DOI:10.1016/j.jinf.2025.106608

Muhammad Bashir Bello , Zainab BuAli , Nidia S. Trovao , Safia S. Aljedani , Abdullah Algaissi , Khalid J. Shrwani , Samer Zakari , Sharif Hala , Rfeef Alyami , Mohammad Bosaeed

{"title":"登革病毒在沙特阿拉伯反复传入和隐传的分子进化见解","authors":"Muhammad Bashir Bello , Zainab BuAli , Nidia S. Trovao , Safia S. Aljedani , Abdullah Algaissi , Khalid J. Shrwani , Samer Zakari , Sharif Hala , Rfeef Alyami , Mohammad Bosaeed","doi":"10.1016/j.jinf.2025.106608","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>To investigate the genetic diversity, evolutionary dynamics, and phylogeography of DENV strains circulating in Saudi Arabia.</div></div><div><h3>Methods</h3><div>We conducted serotyping, whole-genome sequencing, and phylogeographic analyses of DENV strains collected across Saudi Arabia between 2021 and 2023. A total of 20 full genomes were successfully obtained: DENV-1 (n = 2), DENV-2 (n = 10), and DENV-3 (n = 8).</div></div><div><h3>Results</h3><div>Serotyping revealed co-circulation of DENV-1, DENV-2, and DENV-3, with DENV-2 emerging as the predominant serotype. Phylogeographic analysis of whole genomes identified at least five distinct introductions of DENV-2 genotype II into Saudi Arabia, primarily originating from India, Sri Lanka and Pakistan. The earliest introduction was estimated around 13 June 1985 (95% HPD: 5 June 1983 to 11 September 1986). DENV-1 genotype III, undetected for over two decades, re-emerged in Jazan and was likely introduced from Djibouti (TMRCA: 27 July 2018; 95% HPD: 9 December 2017 to 21 March 2019). Two independent introductions of DENV-3 genotype III were identified, originating from Malaysia and India, with TMRCA estimates ranging from 2007 to 2011—indicating at least a decade of undetected circulation.</div></div><div><h3>Conclusions</h3><div>Our findings highlight Saudi Arabia’s evolving role as a regional hub for DENV transmission, driven by mass gatherings and labor migration. Strengthening genomic surveillance, enhancing vector control, and fostering regional data sharing are critical to improving outbreak response and preparedness.</div></div>","PeriodicalId":50180,"journal":{"name":"Journal of Infection","volume":"91 3","pages":"Article 106608"},"PeriodicalIF":11.9000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular evolutionary insights into the repeated introductions and cryptic transmission of dengue virus in Saudi Arabia\",\"authors\":\"Muhammad Bashir Bello , Zainab BuAli , Nidia S. Trovao , Safia S. Aljedani , Abdullah Algaissi , Khalid J. Shrwani , Samer Zakari , Sharif Hala , Rfeef Alyami , Mohammad Bosaeed\",\"doi\":\"10.1016/j.jinf.2025.106608\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>To investigate the genetic diversity, evolutionary dynamics, and phylogeography of DENV strains circulating in Saudi Arabia.</div></div><div><h3>Methods</h3><div>We conducted serotyping, whole-genome sequencing, and phylogeographic analyses of DENV strains collected across Saudi Arabia between 2021 and 2023. A total of 20 full genomes were successfully obtained: DENV-1 (n = 2), DENV-2 (n = 10), and DENV-3 (n = 8).</div></div><div><h3>Results</h3><div>Serotyping revealed co-circulation of DENV-1, DENV-2, and DENV-3, with DENV-2 emerging as the predominant serotype. Phylogeographic analysis of whole genomes identified at least five distinct introductions of DENV-2 genotype II into Saudi Arabia, primarily originating from India, Sri Lanka and Pakistan. The earliest introduction was estimated around 13 June 1985 (95% HPD: 5 June 1983 to 11 September 1986). DENV-1 genotype III, undetected for over two decades, re-emerged in Jazan and was likely introduced from Djibouti (TMRCA: 27 July 2018; 95% HPD: 9 December 2017 to 21 March 2019). Two independent introductions of DENV-3 genotype III were identified, originating from Malaysia and India, with TMRCA estimates ranging from 2007 to 2011—indicating at least a decade of undetected circulation.</div></div><div><h3>Conclusions</h3><div>Our findings highlight Saudi Arabia’s evolving role as a regional hub for DENV transmission, driven by mass gatherings and labor migration. Strengthening genomic surveillance, enhancing vector control, and fostering regional data sharing are critical to improving outbreak response and preparedness.</div></div>\",\"PeriodicalId\":50180,\"journal\":{\"name\":\"Journal of Infection\",\"volume\":\"91 3\",\"pages\":\"Article 106608\"},\"PeriodicalIF\":11.9000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Infection\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0163445325002087\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Infection","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0163445325002087","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

摘要

研究沙特阿拉伯流行的DENV毒株的遗传多样性、进化动态和系统地理。方法对2021年至2023年在沙特阿拉伯收集的DENV菌株进行血清分型、全基因组测序和系统地理分析。共获得20个全基因组：DENV-1 （n = 2）、DENV-2 （n = 10）和DENV-3 （n = 8）。结果血清分型显示DENV-1、DENV-2和DENV-3共循环，其中DENV-2为主要血清型。对全基因组的系统地理分析发现，至少有5种不同的DENV-2基因型传入沙特阿拉伯，主要源自印度、斯里兰卡和巴基斯坦。最早的引进估计在1985年6月13日左右（95% HPD: 1983年6月5日至1986年9月11日）。20多年来未被发现的DENV-1基因III型在吉赞再次出现，可能是从吉布提传入的（TMRCA: 2018年7月27日；95% HPD: 2017年12月9日至2019年3月21日）。发现了两个独立的DENV-3基因型引进，分别来自马来西亚和印度，TMRCA估计时间为2007年至2011年，表明至少有十年未被发现的传播。研究结果表明，在大规模集会和劳动力迁移的推动下，沙特阿拉伯正在成为DENV传播的区域中心。加强基因组监测、加强病媒控制和促进区域数据共享对于改善疫情应对和防范至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Molecular evolutionary insights into the repeated introductions and cryptic transmission of dengue virus in Saudi Arabia

Background

To investigate the genetic diversity, evolutionary dynamics, and phylogeography of DENV strains circulating in Saudi Arabia.

Methods

We conducted serotyping, whole-genome sequencing, and phylogeographic analyses of DENV strains collected across Saudi Arabia between 2021 and 2023. A total of 20 full genomes were successfully obtained: DENV-1 (n = 2), DENV-2 (n = 10), and DENV-3 (n = 8).

Results

Serotyping revealed co-circulation of DENV-1, DENV-2, and DENV-3, with DENV-2 emerging as the predominant serotype. Phylogeographic analysis of whole genomes identified at least five distinct introductions of DENV-2 genotype II into Saudi Arabia, primarily originating from India, Sri Lanka and Pakistan. The earliest introduction was estimated around 13 June 1985 (95% HPD: 5 June 1983 to 11 September 1986). DENV-1 genotype III, undetected for over two decades, re-emerged in Jazan and was likely introduced from Djibouti (TMRCA: 27 July 2018; 95% HPD: 9 December 2017 to 21 March 2019). Two independent introductions of DENV-3 genotype III were identified, originating from Malaysia and India, with TMRCA estimates ranging from 2007 to 2011—indicating at least a decade of undetected circulation.

Conclusions

Our findings highlight Saudi Arabia’s evolving role as a regional hub for DENV transmission, driven by mass gatherings and labor migration. Strengthening genomic surveillance, enhancing vector control, and fostering regional data sharing are critical to improving outbreak response and preparedness.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Infection 医学-传染病学

CiteScore

45.90

自引率

3.20%

发文量

475

审稿时长

16 days

期刊介绍： The Journal of Infection publishes original papers on all aspects of infection - clinical, microbiological and epidemiological. The Journal seeks to bring together knowledge from all specialties involved in infection research and clinical practice, and present the best work in the ever-changing field of infection. Each issue brings you Editorials that describe current or controversial topics of interest, high quality Reviews to keep you in touch with the latest developments in specific fields of interest, an Epidemiology section reporting studies in the hospital and the general community, and a lively correspondence section.