用商业ELISA快速通道法定量食品行业原料金枪鱼罐头中的组胺

IF 2.8 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2025-09-01 DOI:10.1016/j.jfp.2025.100602

Luigi Crobu , Valeria Pasciu , Eleonora Serra , Gonzalo Crosi Martinez , Lucia Trujillo , Rita Melillo , Giuseppe Esposito , Domenico Meloni

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引用次数: 0

摘要

组胺的存在通常被用作良好生产规范和罐装金枪鱼保存状态的指标。本产品在饮食业广泛用作配料，经常出现不正确的储存条件和可能的后处理污染，导致组胺的产生。在本研究中，我们应用了一种快速ELISA筛选方法，用于定量测定作为餐饮行业配料的金枪鱼罐头中的组胺。一项实验是在打开的金枪鱼罐头上进行的，并在冷藏温度下保存长达一周，以模拟“最坏情况”的储存条件。总的来说，结果明显低于欧盟法规2073/2005规定的食品安全标准（组胺含量为100 mg/kg）；然而，ELISA筛选试验显示，在“最坏”条件下储存的样品中，组胺水平有统计学意义（p < 0.00001）的增加。HistaSure™ELISA快速通道方法具有很大的优势，可以很容易地作为HACCP应用中的一种可能的控制策略。

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Quantification of Histamine in Canned Tuna Used as an Ingredient in the Catering Trade by a Commercial ELISA Fast-Track Method

The presence of Histamine is generally used as an indicator of the good manufacturing practice and of the preservation state of canned tuna. This product is widely used as an ingredient in the catering trade with frequent incorrect storage conditions and likely postprocessing contamination causing Histamine production. In this study, we have applied a rapid ELISA screening method for the quantitative determination of Histamine in canned tuna used as an ingredient in the catering trade. An experiment was carried out on tuna cans opened and held at refrigeration temperatures for up to one week as a simulation of ‘‘worst-case’’ storage conditions. Altogether, the results were markedly under the food safety criteria indicated by EC Regulation 2073/2005 (<100 mg/kg of histamine); however, the ELISA screening test revealed a statistically significant (p < 0.00001) increase of the Histamine levels in samples stored in ‘‘worst-case’’ conditions. The HistaSure™ ELISA Fast Track method offered great advantages and can be easily used as a possible control strategy in HACCP applications.

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来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.