[靶向溶酶体相关膜蛋白5的miR-302a-3p抑制口腔鳞状细胞癌的侵袭转移]。

Li Yu, Tiejun Zhou, Xiao Wu, Xinhong Lin, Xiaoyan Zhang, Yongxian Lai, Xinyue Liao, Hang Si, Yun Feng, Jie Jian, Yan Feng
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引用次数: 0

摘要

目的:本研究旨在探讨溶酶体相关膜蛋白5 (LAMP5)和microRNA (miR)-302a-3p在口腔鳞癌(OSCC)中的表达及其在OSCC侵袭转移中的作用机制。方法:基于The Cancer Genome Atlas数据库,分析LAMP5在OSCC中的表达及其作为预后指标的敏感性。采用Western blot、定量逆转录聚合酶链反应和细胞免疫细胞化学检测LAMP5在OSCC组织和细胞中的表达。通过细胞计数试剂盒-8、免疫细胞化学、迁移和侵袭实验分别评估LAMP5对OSCC细胞增殖、迁移和侵袭的影响。利用miRNA靶向预测网站预测调控LAMP5的miR,验证miR-302a-3p对LAMP5的靶向调控作用。在裸鼠肿瘤发生模型中评估了LAMP5敲低对OSCC肿瘤生长的影响。结果:LAMP5在OSCC组织和细胞中高表达。对OSCC的早期诊断具有较高的敏感性。LAMP5敲低可显著抑制OSCC细胞的增殖、迁移和侵袭,而LAMP5过表达可增加这些细胞的活性。LAMP5的表达受miR-302a-3p调控。在体内,LAMP5敲低可显著抑制OSCC肿瘤的生长。结论:LAMP5通过增强OSCC细胞的增殖、迁移和侵袭,促进OSCC的恶性进展。miR-302a-3p负调控LAMP5的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[miR-302a-3p targeting lysosomal-associated membrane protein 5 inhibits the invasion and metastasis of oral squamous cell carcinoma].

Objectives: This study aimed to explore the expression of lysosomal-associated membrane protein 5 (LAMP5) and microRNA (miR)-302a-3p in oral squamous cell carcinoma (OSCC) and their functional mechanism on the invasion and metastasis of OSCC.

Methods: The expression of LAMP5 in OSCC and its sensitivity as a prognostic indicator were analyzed on the basis of The Cancer Genome Atlas database. Western blot, quantitative reverse transcription polymerase chain reaction, and cell immunocytochemistry were used to detect the expression of LAMP5 in OSCC tissues and cells. The effect of LAMP5 on the proliferation, migration, and invasion of OSCC cells was evaluated through cell counting kit-8, immunocytochemistry, migration, and invasion assays, respectively. The miRNA targeting prediction websites were used to predict the miR that regulates LAMP5 and verify the targeted regulatory effect of miR-302a-3p on LAMP5. The effect of LAMP5 knockdown on OSCC tumor growth was evaluated in a nude mouse tumorigenesis model.

Results: LAMP5 was highly expressed in OSCC tissues and cells. It showed high sensitivity in the early diagnosis of OSCC. LAMP5 knockdown significantly inhibited the proliferation, migration, and invasion of OSCC cells, whereas LAMP5 overexpression increased these cell activities. The expression of LAMP5 was regulated by miR-302a-3p. In vivo, LAMP5 knockdown significantly inhibited the growth of OSCC tumor.

Conclusions: LAMP5 promotes the malignant progression of OSCC by enhancing the proliferation, migration, and invasion of OSCC cells. The expression of LAMP5 is negatively regulated by miR-302a-3p.

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