一种用于活细胞成像和CFTR活性评价的选择性碘化指示剂的研制。

IF 4.1 Q2 CHEMISTRY, ANALYTICAL

Sensors & diagnostics Pub Date : 2025-08-28 DOI:10.1039/D5SD00086F

Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang and Kaho Leung

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引用次数: 0

摘要

囊性纤维化（CF）是由囊性纤维化跨膜传导调节基因（CFTR）突变引起的。监测I-转运是评估活细胞中CFTR功能的关键方法，为诊断工具和治疗方法的发展提供了基础。在这里，我们报道了一种铱（iii）复合物（I- sense），用于选择性和ph无关的细胞内I-成像。通过跟踪细胞碘I-摄取，I- sense促进了活细胞中CFTR活性的评估，为CFTR活性的功能表征提供了有价值的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity

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Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity

Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I⁻ transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(III) complex (I-Sense) for the selective and pH-independent imaging of intracellular I⁻. By tracking cellular iodide I⁻ uptake, I-Sense facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.

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来源期刊

Sensors & diagnostics

CiteScore

2.30

自引率

0.00%

发文量