Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang and Kaho Leung
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Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity
Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I− transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(III) complex (I-Sense) for the selective and pH-independent imaging of intracellular I−. By tracking cellular iodide I− uptake, I-Sense facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.