{"title":"一种用于活细胞成像和CFTR活性评价的选择性碘化指示剂的研制。","authors":"Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang, Kaho Leung","doi":"10.1039/d5sd00086f","DOIUrl":null,"url":null,"abstract":"<p><p>Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I<sup>-</sup> transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(iii) complex (<b>I-Sense</b>) for the selective and pH-independent imaging of intracellular I<sup>-</sup>. By tracking cellular iodide I<sup>-</sup> uptake, <b>I-Sense</b> facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" ","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394795/pdf/","citationCount":"0","resultStr":"{\"title\":\"Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity.\",\"authors\":\"Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang, Kaho Leung\",\"doi\":\"10.1039/d5sd00086f\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I<sup>-</sup> transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(iii) complex (<b>I-Sense</b>) for the selective and pH-independent imaging of intracellular I<sup>-</sup>. By tracking cellular iodide I<sup>-</sup> uptake, <b>I-Sense</b> facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.</p>\",\"PeriodicalId\":74786,\"journal\":{\"name\":\"Sensors & diagnostics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394795/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensors & diagnostics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1039/d5sd00086f\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/d5sd00086f","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity.
Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I- transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(iii) complex (I-Sense) for the selective and pH-independent imaging of intracellular I-. By tracking cellular iodide I- uptake, I-Sense facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.
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