Stephen M Kotfila, Sarah J Leduc, Genna M Mullen, Shawna C O Reed
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引用次数: 0
摘要
伯纳蒂克希菌是一种革兰氏阴性的专性细胞内细菌病原体，可引起人类人畜共患Q热。在哺乳动物宿主中，伯氏梭菌可感染巨噬细胞、心脏、大脑、肝脏和胎盘细胞。为了研究，通常在HeLa（人类宫颈）和Vero（非洲绿猴肾）细胞中培养伯纳蒂胞杆菌，但这些细胞类型不能很好地反映自然的复制生态位。在这里，我们报告了burnetii， Nine Mile II期，在N2A（小鼠神经元），HepG2（人肝细胞）和MCF7（人乳腺上皮）细胞中复制的第一个证据。这些发现将为进一步比较研究不同细胞类型的伯氏梭菌的细胞病理学和感染动力学提供依据。
N2A, MCF7, and HepG2 Cells Support Intracellular Replication of Coxiella burnetii.
Coxiella burnetii is a gram-negative, obligate, intracellular bacterial pathogen that causes zoonotic Q fever in humans. In a mammalian host, C. burnetii may infect macrophage, heart, brain, liver, and placental cells. C. burnetii is routinely cultured in HeLa (human cervical) and Vero (African green monkey kidney) cells for research, but these cell types poorly reflect the natural replicative niche. Here we report the first evidence of C. burnetii , Nine Mile Phase II, replication in N2A (mouse neuronal), HepG2 (human hepatocyte), and MCF7 (human mammary epithelial) cells. These findings will enable further comparative study of C. burnetii cytopathology and infection dynamics in various cell types.
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