Oscar de Souza Lopes , Suely S.D. de Almeida Guimarães , Ricardo de Carvalho
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引用次数: 6
摘要
这项工作评估了稀释黄热病疫苗的稳定性，以确定维持世卫组织要求的每人剂量至少3 log10的17D病毒的条件。将疫苗保存在0°C或37°C，用蒸馏水、0.15 m生理盐水或0.15 m PBS (pH值为5.5、7.2和8.0)稀释。在下一步中，稳定物质如明胶和蛋白胨被添加到疫苗中。将疫苗用蒸馏水稀释后，在37°C下可将病毒滴度维持3小时，巴西已采用这种稀释剂作为常规使用。
Studies on yellow fever vaccine II—stability of the reconstituted product
This work evaluated the stability of diluted yellow fever vaccine in order to determine conditions that maintain the minimum of 3 log10 of 17D virus per human dose as required by WHO. The vaccines were held at 0 °C or at 37 °C and were diluted either with distilled water, with 0·15 m saline or with 0·15 m PBS at pH 5·5, 7·2 and 8·0. In a next step, stabilizer substances such as gelatin and peptone were added to the vaccines. Dilution of the vaccines in distilled water maintained the virus titre for up to three hours at 37 °C and this diluent has been adopted for routine use in Brazil.
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