鉴定儿童脉络丛乳头状瘤中的血管生成因子。

IF 2 Q3 CLINICAL NEUROLOGY
NeuroSci Pub Date : 2025-08-11 DOI:10.3390/neurosci6030076
Nurfarhanah Bte Syed Sulaiman, Sofiah M Y Sng, Khurshid Z Merchant, Lee Ping Ng, David C Y Low, Wan Tew Seow, Sharon Y Y Low
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引用次数: 0

摘要

(1)背景:脉络丛乳头状瘤(CPPs)是一种罕见的脑肿瘤,多发生于幼童。CPP发展的机制尚不清楚。另外,血管生成的过程也与其他原发性脑肿瘤有关。我们假设血管生成是CPP生物学的一个标志。本研究旨在鉴定和验证CPPs中的血管生成因子。(2)方法:采集脑脊液(CSF)和CPP肿瘤标本。多重免疫测定板用于鉴定CSF样品中差异表达的细胞因子。同时,患者来源的原代细胞培养物及其上清液来自CPP样品。靶向蛋白质组印迹阵列和人脐静脉内皮细胞(HUVEC)血管生成测定用于验证研究。(3)结果:脑脊液分析显示,与非肿瘤对照相比,CPP患者样本中VEGF-A、MCP-1、MMP-1、TNF-α和CD40L的表达更高。接下来,通过在线蛋白-蛋白网络平台的评估报告,这些细胞因子与内皮细胞调节有关。采用以血管生成为重点的方法,cpp衍生的细胞系和上清液显示出类似的VEGF, MCP-1和MMP-1的高表达。接下来,当加入条件CPP细胞培养基时,观察HUVEC血管生成实验培养中淋巴结的发芽和小管的形成。(4)结论:这项概念验证研究显示了探索CPP血管生成的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identifying Angiogenic Factors in Pediatric Choroid Plexus Papillomas.

Identifying Angiogenic Factors in Pediatric Choroid Plexus Papillomas.

Identifying Angiogenic Factors in Pediatric Choroid Plexus Papillomas.

Identifying Angiogenic Factors in Pediatric Choroid Plexus Papillomas.

(1) Background: Choroid plexus papillomas (CPPs) are rare brain tumors that tend to occur in very young children. Mechanisms of CPP development remain unelucidated. Separately, the process of angiogenesis has been implicated in other primary brain tumors. We hypothesize that angiogenesis is a hallmark of CPP biology. This study aims to identify and validate angiogenic factors in CPPs. (2) Methods: Cerebrospinal fluid (CSF) and CPP tumor samples are collected. A multiplex immunoassay panel is used to identify differentially expressed cytokines in the CSF samples. Concurrently, patient-derived primary cell cultures and their supernatants are derived from CPP samples. Targeted proteome blot arrays and human umbilical vein endothelial cell (HUVEC) angiogenesis assays are used for validation studies. (3) Results: CSF profiling showed higher expressions of VEGF-A, MCP-1, MMP-1, TNF-α, and CD40L in CPP patient samples versus non-tumor controls. Next, assessment via online protein-protein network platforms reports that these cytokines are associated with endothelial cell regulation. Using an angiogenesis-focused approach, CPP-derived cell lines and supernatants showed similarly higher expressions of VEGF, MCP-1, and MMP-1. Next, sprouting of nodes and tubule formation were observed in HUVEC angiogenesis assay cultures when conditioned CPP cell culture media was added. (4) Conclusions: This proof-of-concept study demonstrates potential to explore angiogenesis in CPP.

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