Mukharram M Bikbov, Gyulli M Kazakbaeva, Songhomitra Panda-Jonas, Iskander D Valishin, Aigul M Nizamutdinova, Jost B Jonas
{"title":"玻璃体内帕尼单抗与激光诱导兔视网膜变性视网膜色素上皮增殖的关系。","authors":"Mukharram M Bikbov, Gyulli M Kazakbaeva, Songhomitra Panda-Jonas, Iskander D Valishin, Aigul M Nizamutdinova, Jost B Jonas","doi":"10.3389/fopht.2025.1641194","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to examine the effect of intravitreally applied epidermal growth factor (EGF) receptor blocker panitumumab on the proliferation of retinal pigment epithelium cells (RPE) in an experimental model of localized retinal degeneration.</p><p><strong>Methods: </strong>The experimental study included rabbits with age of 2 to 3 months and body weight of 2.5-3 kg and which were randomly distributed into a study group and control group. The right eyes received two retinal argon laser coagulation spots (500 mW; diameter, 100 μm; duration, 0.5 s), applied with an interval of 2 min at the same location close to the vascular streak in the posterior fundus region. For five times at 2-day intervals, the rabbits of the study group received intravitreal injections of 1 mg panitumumab (0.10 mL), and the rabbits of the control group had intravitreal injections of 0.10 mL Ringer's solution. At baseline, at each time point of re-examination, and at study end, the animals were examined by fundus photography and optical coherence tomography of the laser spot.</p><p><strong>Results: </strong>The study included 19 rabbits (study group: 10 animals; control group: nine animals). After the third injection and at study end, the laser-induced area of depigmentation + hyperpigmentation combined did not vary significantly between the study group and the control group (1.43 ± 0.63 mm<sup>2</sup> versus 1.63 ± 0.77 mm<sup>2</sup>; <i>P</i> = 0.56; and 1.37 ± 0.63 mm<sup>2</sup> versus 1.61 ± 0.74 mm<sup>2</sup>; <i>P</i> = 0.46, respectively). At the same time points, the area with hyperpigmentation was significantly smaller in the study group than in the control group (0.16 ± 0.15 mm<sup>2</sup> versus 0.80 ± 0.59 mm<sup>2</sup>; <i>P</i> = 0.01; and 0.14 ± 0.14 mm<sup>2</sup> versus 0.70 ± 0.56 mm<sup>2</sup>; <i>P</i> = 0.02, respectively). At the same time points, the ratio of the hyperpigmented area to the combined depigmented + hyperpigmented area was significantly smaller in the study group than in the control group (0.11 ± 0.09 versus 0.43 ± 0.19 mm<sup>2</sup>; <i>P</i> < 0.001; and 0.10 ± 0.08 versus 0.35 ± 0.23mm<sup>2</sup>; <i>P</i> = 0.006, respectively).</p><p><strong>Conclusions: </strong>These findings indicate that intravitreally administered panitumumab was associated with reduced subretinal hyperpigmentation in a laser-induced model of retinal injury. While this may reflect a modulation of the RPE response, including the potential suppression of RPE proliferation, further studies incorporating histological and molecular analyses are warranted to confirm its effect on subretinal fibrosis.</p>","PeriodicalId":73096,"journal":{"name":"Frontiers in ophthalmology","volume":"5 ","pages":"1641194"},"PeriodicalIF":0.9000,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367493/pdf/","citationCount":"0","resultStr":"{\"title\":\"Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits.\",\"authors\":\"Mukharram M Bikbov, Gyulli M Kazakbaeva, Songhomitra Panda-Jonas, Iskander D Valishin, Aigul M Nizamutdinova, Jost B Jonas\",\"doi\":\"10.3389/fopht.2025.1641194\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>This study aims to examine the effect of intravitreally applied epidermal growth factor (EGF) receptor blocker panitumumab on the proliferation of retinal pigment epithelium cells (RPE) in an experimental model of localized retinal degeneration.</p><p><strong>Methods: </strong>The experimental study included rabbits with age of 2 to 3 months and body weight of 2.5-3 kg and which were randomly distributed into a study group and control group. The right eyes received two retinal argon laser coagulation spots (500 mW; diameter, 100 μm; duration, 0.5 s), applied with an interval of 2 min at the same location close to the vascular streak in the posterior fundus region. For five times at 2-day intervals, the rabbits of the study group received intravitreal injections of 1 mg panitumumab (0.10 mL), and the rabbits of the control group had intravitreal injections of 0.10 mL Ringer's solution. At baseline, at each time point of re-examination, and at study end, the animals were examined by fundus photography and optical coherence tomography of the laser spot.</p><p><strong>Results: </strong>The study included 19 rabbits (study group: 10 animals; control group: nine animals). After the third injection and at study end, the laser-induced area of depigmentation + hyperpigmentation combined did not vary significantly between the study group and the control group (1.43 ± 0.63 mm<sup>2</sup> versus 1.63 ± 0.77 mm<sup>2</sup>; <i>P</i> = 0.56; and 1.37 ± 0.63 mm<sup>2</sup> versus 1.61 ± 0.74 mm<sup>2</sup>; <i>P</i> = 0.46, respectively). At the same time points, the area with hyperpigmentation was significantly smaller in the study group than in the control group (0.16 ± 0.15 mm<sup>2</sup> versus 0.80 ± 0.59 mm<sup>2</sup>; <i>P</i> = 0.01; and 0.14 ± 0.14 mm<sup>2</sup> versus 0.70 ± 0.56 mm<sup>2</sup>; <i>P</i> = 0.02, respectively). At the same time points, the ratio of the hyperpigmented area to the combined depigmented + hyperpigmented area was significantly smaller in the study group than in the control group (0.11 ± 0.09 versus 0.43 ± 0.19 mm<sup>2</sup>; <i>P</i> < 0.001; and 0.10 ± 0.08 versus 0.35 ± 0.23mm<sup>2</sup>; <i>P</i> = 0.006, respectively).</p><p><strong>Conclusions: </strong>These findings indicate that intravitreally administered panitumumab was associated with reduced subretinal hyperpigmentation in a laser-induced model of retinal injury. While this may reflect a modulation of the RPE response, including the potential suppression of RPE proliferation, further studies incorporating histological and molecular analyses are warranted to confirm its effect on subretinal fibrosis.</p>\",\"PeriodicalId\":73096,\"journal\":{\"name\":\"Frontiers in ophthalmology\",\"volume\":\"5 \",\"pages\":\"1641194\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367493/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in ophthalmology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3389/fopht.2025.1641194\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in ophthalmology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/fopht.2025.1641194","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:本研究旨在探讨玻璃体内应用表皮生长因子(EGF)受体阻滞剂帕尼单抗对局限性视网膜变性实验模型视网膜色素上皮细胞(RPE)增殖的影响。方法:选取2 ~ 3月龄、体重2.5 ~ 3 kg的家兔,随机分为研究组和对照组。右眼眼底后区血管纹附近同一位置接受2个视网膜氩激光凝固斑(直径500 mW,直径100 μm,持续时间0.5 s),间隔2 min。研究组兔玻璃体内注射1 mg panitumumab (0.10 mL),每隔2天注射5次,对照组兔玻璃体内注射0.10 mL林格氏液。在基线、每个时间点复查和研究结束时,对动物进行眼底摄影和激光光斑光学相干断层扫描。结果:共纳入实验兔19只(研究组10只,对照组9只)。在第三次注射后和研究结束时,激光诱导的脱色+色素沉着合并面积在研究组和对照组之间无显著差异(1.43±0.63 mm2 vs 1.63±0.77 mm2, P = 0.56; 1.37±0.63 mm2 vs 1.61±0.74 mm2, P = 0.46)。在同一时间点,研究组色素沉着面积明显小于对照组(0.16±0.15 mm2 vs 0.80±0.59 mm2, P = 0.01; 0.14±0.14 mm2 vs 0.70±0.56 mm2, P = 0.02)。在同一时间点,研究组的色素沉着面积与脱色+色素沉着合并面积之比明显小于对照组(分别为0.11±0.09比0.43±0.19 mm2, P < 0.001; 0.10±0.08比0.35±0.23mm2, P = 0.006)。结论:这些发现表明,在激光诱导的视网膜损伤模型中,玻璃体内给药panitumumab与视网膜下色素沉着减少有关。虽然这可能反映了RPE反应的调节,包括对RPE增殖的潜在抑制,但需要进一步的研究结合组织学和分子分析来证实其对视网膜下纤维化的影响。
Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits.
Purpose: This study aims to examine the effect of intravitreally applied epidermal growth factor (EGF) receptor blocker panitumumab on the proliferation of retinal pigment epithelium cells (RPE) in an experimental model of localized retinal degeneration.
Methods: The experimental study included rabbits with age of 2 to 3 months and body weight of 2.5-3 kg and which were randomly distributed into a study group and control group. The right eyes received two retinal argon laser coagulation spots (500 mW; diameter, 100 μm; duration, 0.5 s), applied with an interval of 2 min at the same location close to the vascular streak in the posterior fundus region. For five times at 2-day intervals, the rabbits of the study group received intravitreal injections of 1 mg panitumumab (0.10 mL), and the rabbits of the control group had intravitreal injections of 0.10 mL Ringer's solution. At baseline, at each time point of re-examination, and at study end, the animals were examined by fundus photography and optical coherence tomography of the laser spot.
Results: The study included 19 rabbits (study group: 10 animals; control group: nine animals). After the third injection and at study end, the laser-induced area of depigmentation + hyperpigmentation combined did not vary significantly between the study group and the control group (1.43 ± 0.63 mm2 versus 1.63 ± 0.77 mm2; P = 0.56; and 1.37 ± 0.63 mm2 versus 1.61 ± 0.74 mm2; P = 0.46, respectively). At the same time points, the area with hyperpigmentation was significantly smaller in the study group than in the control group (0.16 ± 0.15 mm2 versus 0.80 ± 0.59 mm2; P = 0.01; and 0.14 ± 0.14 mm2 versus 0.70 ± 0.56 mm2; P = 0.02, respectively). At the same time points, the ratio of the hyperpigmented area to the combined depigmented + hyperpigmented area was significantly smaller in the study group than in the control group (0.11 ± 0.09 versus 0.43 ± 0.19 mm2; P < 0.001; and 0.10 ± 0.08 versus 0.35 ± 0.23mm2; P = 0.006, respectively).
Conclusions: These findings indicate that intravitreally administered panitumumab was associated with reduced subretinal hyperpigmentation in a laser-induced model of retinal injury. While this may reflect a modulation of the RPE response, including the potential suppression of RPE proliferation, further studies incorporating histological and molecular analyses are warranted to confirm its effect on subretinal fibrosis.