无价HPV疫苗在hiv感染妇女中靶向的致癌HPV基因型的高流行率迫切地加强了对生活在加蓬的主要女性人群进行预防性疫苗接种的必要性。

IF 3 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Marcelle Mboumba-Mboumba, Augustin Mouinga-Ondeme, Pamela Moussavou-Boundzanga, Jeordy Dimitri Engone-Ondo, Roseanne Mounanga Mourimarodi, Abdoulaye Diane, Christ Ognari Ayoumi, Laurent Bélec, Ralph-Sydney Mboumba Bouassa, Ivan Mfouo-Tynga
{"title":"无价HPV疫苗在hiv感染妇女中靶向的致癌HPV基因型的高流行率迫切地加强了对生活在加蓬的主要女性人群进行预防性疫苗接种的必要性。","authors":"Marcelle Mboumba-Mboumba, Augustin Mouinga-Ondeme, Pamela Moussavou-Boundzanga, Jeordy Dimitri Engone-Ondo, Roseanne Mounanga Mourimarodi, Abdoulaye Diane, Christ Ognari Ayoumi, Laurent Bélec, Ralph-Sydney Mboumba Bouassa, Ivan Mfouo-Tynga","doi":"10.3390/diseases13080260","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background/Objectives.</b> Women living with human immunodeficiency virus (WLWH) have a six-fold higher risk of developing cervical cancer associated with high-risk human Papillomavirus (HR-HPV) than HIV-negative women. We herein assessed HR-HPV genotype distribution and plasma levels of the cancer antigen 125 (CA-125) in WLWH in a rural town in Gabon, in Central Africa. <b>Methods.</b> Adult WLWH attending the local HIV outpatient center were prospectively enrolled and underwent cervical visual inspection and cervicovaginal and blood sampling. HIV RNA load and CA-125 levels were measured from plasma using the Cepheid<sup>®</sup> Xpert<sup>®</sup> HIV-1 Viral Load kit and BioMérieux VIDAS<sup>®</sup> CA-125 II assay, respectively. HPV detection and genotyping were performed via a nested polymerase chain reaction (MY09/11 and GP5+/6+), followed by sequencing. <b>Results.</b> Fifty-eight WLWH (median age: 52 years) were enrolled. Median CD4 count was 547 cells/µL (IQR: 412.5-737.5) and HIV RNA load 4.88 Log<sub>10</sub> copies/mL (IQR: 3.79-5.49). HPV prevalence was 68.96%, with HR-HPV detected in 41.37% of women. Among HR-HPV-positive samples, 87.5% (21/24) were genotypes targeted by the Gardasil vaccine, while 12.5% (3/24) were non-vaccine types. Predominant HR-HPV types included HPV-16 (13.8%), HPV-33 (10.34%), HPV-35 (5.17%), HPV-31, and HPV-58 (3.45%). Most participants had normal cervical cytology (62.07%), and a minority (14.29%) had elevated CA-125 levels, with no correlation to cytological abnormalities. <b>Conclusions.</b> In the hinterland of Gabon, WLWH are facing an unsuspected yet substantial burden of cervical HR-HPV infection and a neglected risk for cervical cancer. Strengthening cervical cancer prevention through targeted HPV vaccination, sexual education, and accessible screening strategies will help in mitigating associated risk.</p>","PeriodicalId":72832,"journal":{"name":"Diseases (Basel, Switzerland)","volume":"13 8","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12386138/pdf/","citationCount":"0","resultStr":"{\"title\":\"The High Prevalence of Oncogenic HPV Genotypes Targeted by the Nonavalent HPV Vaccine in HIV-Infected Women Urgently Reinforces the Need for Prophylactic Vaccination in Key Female Populations Living in Gabon.\",\"authors\":\"Marcelle Mboumba-Mboumba, Augustin Mouinga-Ondeme, Pamela Moussavou-Boundzanga, Jeordy Dimitri Engone-Ondo, Roseanne Mounanga Mourimarodi, Abdoulaye Diane, Christ Ognari Ayoumi, Laurent Bélec, Ralph-Sydney Mboumba Bouassa, Ivan Mfouo-Tynga\",\"doi\":\"10.3390/diseases13080260\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Background/Objectives.</b> Women living with human immunodeficiency virus (WLWH) have a six-fold higher risk of developing cervical cancer associated with high-risk human Papillomavirus (HR-HPV) than HIV-negative women. We herein assessed HR-HPV genotype distribution and plasma levels of the cancer antigen 125 (CA-125) in WLWH in a rural town in Gabon, in Central Africa. <b>Methods.</b> Adult WLWH attending the local HIV outpatient center were prospectively enrolled and underwent cervical visual inspection and cervicovaginal and blood sampling. HIV RNA load and CA-125 levels were measured from plasma using the Cepheid<sup>®</sup> Xpert<sup>®</sup> HIV-1 Viral Load kit and BioMérieux VIDAS<sup>®</sup> CA-125 II assay, respectively. HPV detection and genotyping were performed via a nested polymerase chain reaction (MY09/11 and GP5+/6+), followed by sequencing. <b>Results.</b> Fifty-eight WLWH (median age: 52 years) were enrolled. Median CD4 count was 547 cells/µL (IQR: 412.5-737.5) and HIV RNA load 4.88 Log<sub>10</sub> copies/mL (IQR: 3.79-5.49). HPV prevalence was 68.96%, with HR-HPV detected in 41.37% of women. Among HR-HPV-positive samples, 87.5% (21/24) were genotypes targeted by the Gardasil vaccine, while 12.5% (3/24) were non-vaccine types. Predominant HR-HPV types included HPV-16 (13.8%), HPV-33 (10.34%), HPV-35 (5.17%), HPV-31, and HPV-58 (3.45%). Most participants had normal cervical cytology (62.07%), and a minority (14.29%) had elevated CA-125 levels, with no correlation to cytological abnormalities. <b>Conclusions.</b> In the hinterland of Gabon, WLWH are facing an unsuspected yet substantial burden of cervical HR-HPV infection and a neglected risk for cervical cancer. Strengthening cervical cancer prevention through targeted HPV vaccination, sexual education, and accessible screening strategies will help in mitigating associated risk.</p>\",\"PeriodicalId\":72832,\"journal\":{\"name\":\"Diseases (Basel, Switzerland)\",\"volume\":\"13 8\",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-08-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12386138/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diseases (Basel, Switzerland)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/diseases13080260\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diseases (Basel, Switzerland)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/diseases13080260","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0

摘要

背景/目标。感染人类免疫缺陷病毒(WLWH)的妇女患与高危人乳头瘤病毒(HR-HPV)相关的宫颈癌的风险比艾滋病毒阴性妇女高6倍。我们在此评估了中非加蓬农村小镇WLWH中HR-HPV基因型分布和癌症抗原125 (CA-125)的血浆水平。方法。参加当地HIV门诊中心的成年WLWH被前瞻性招募,并接受宫颈视觉检查和宫颈阴道和血液采样。分别使用Cepheid®Xpert®HIV-1病毒载量试剂盒和biomrieux VIDAS®CA-125 II检测血浆中HIV RNA载量和CA-125水平。通过巢式聚合酶链反应(MY09/11和GP5+/6+)进行HPV检测和基因分型,然后进行测序。结果。纳入了58名WLWH(中位年龄:52岁)。中位CD4计数为547个细胞/µL (IQR: 412.5-737.5), HIV RNA载量为4.88 Log10拷贝/mL (IQR: 3.79-5.49)。HPV患病率为68.96%,其中HR-HPV检出率为41.37%。在hr - hpv阳性样本中,87.5%(21/24)为加德西疫苗靶向基因型,12.5%(3/24)为非疫苗型。主要的HR-HPV类型包括HPV-16(13.8%)、HPV-33(10.34%)、HPV-35(5.17%)、HPV-31和HPV-58(3.45%)。大多数参与者宫颈细胞学正常(62.07%),少数(14.29%)CA-125水平升高,与细胞学异常无关。结论。在加蓬腹地,产妇面临着子宫颈人乳头瘤病毒感染这一意料之外的重大负担,以及被忽视的宫颈癌风险。通过有针对性的HPV疫苗接种、性教育和可获得的筛查策略加强宫颈癌预防将有助于减轻相关风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The High Prevalence of Oncogenic HPV Genotypes Targeted by the Nonavalent HPV Vaccine in HIV-Infected Women Urgently Reinforces the Need for Prophylactic Vaccination in Key Female Populations Living in Gabon.

The High Prevalence of Oncogenic HPV Genotypes Targeted by the Nonavalent HPV Vaccine in HIV-Infected Women Urgently Reinforces the Need for Prophylactic Vaccination in Key Female Populations Living in Gabon.

The High Prevalence of Oncogenic HPV Genotypes Targeted by the Nonavalent HPV Vaccine in HIV-Infected Women Urgently Reinforces the Need for Prophylactic Vaccination in Key Female Populations Living in Gabon.

Background/Objectives. Women living with human immunodeficiency virus (WLWH) have a six-fold higher risk of developing cervical cancer associated with high-risk human Papillomavirus (HR-HPV) than HIV-negative women. We herein assessed HR-HPV genotype distribution and plasma levels of the cancer antigen 125 (CA-125) in WLWH in a rural town in Gabon, in Central Africa. Methods. Adult WLWH attending the local HIV outpatient center were prospectively enrolled and underwent cervical visual inspection and cervicovaginal and blood sampling. HIV RNA load and CA-125 levels were measured from plasma using the Cepheid® Xpert® HIV-1 Viral Load kit and BioMérieux VIDAS® CA-125 II assay, respectively. HPV detection and genotyping were performed via a nested polymerase chain reaction (MY09/11 and GP5+/6+), followed by sequencing. Results. Fifty-eight WLWH (median age: 52 years) were enrolled. Median CD4 count was 547 cells/µL (IQR: 412.5-737.5) and HIV RNA load 4.88 Log10 copies/mL (IQR: 3.79-5.49). HPV prevalence was 68.96%, with HR-HPV detected in 41.37% of women. Among HR-HPV-positive samples, 87.5% (21/24) were genotypes targeted by the Gardasil vaccine, while 12.5% (3/24) were non-vaccine types. Predominant HR-HPV types included HPV-16 (13.8%), HPV-33 (10.34%), HPV-35 (5.17%), HPV-31, and HPV-58 (3.45%). Most participants had normal cervical cytology (62.07%), and a minority (14.29%) had elevated CA-125 levels, with no correlation to cytological abnormalities. Conclusions. In the hinterland of Gabon, WLWH are facing an unsuspected yet substantial burden of cervical HR-HPV infection and a neglected risk for cervical cancer. Strengthening cervical cancer prevention through targeted HPV vaccination, sexual education, and accessible screening strategies will help in mitigating associated risk.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.80
自引率
0.00%
发文量
0
审稿时长
6 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信