{"title":"3D基因组测序技术：过去、现在和未来。","authors":"Haoxi Chai, Yijun Ruan","doi":"10.1016/j.tig.2025.07.010","DOIUrl":null,"url":null,"abstract":"<p><p>The genome is packaged with nuclear proteins and RNAs into a complex structure known as chromatin. Its dynamic organization influences genome functions and nuclear properties. Since 2009, high-throughput DNA sequencing methods such as Hi-C and ChIA-PET have pioneered genome-wide mapping of chromatin folding architectures and have given rise to the field of three-dimensional (3D) genome biology. Now, after 15 years of development, this field has experienced a remarkable growth and is still expanding rapidly. It is significantly deepening our understanding of how genome organization affects nuclear functions in various biological systems. In this review we focus on the breakthroughs and expansion of sequencing-based technologies in mapping 3D genomic landscapes and envisage the next frontiers in advancing the 3D genome biology.</p>","PeriodicalId":54413,"journal":{"name":"Trends in Genetics","volume":" ","pages":""},"PeriodicalIF":16.3000,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D genome sequencing technology in its mid-teens: past, present, and future.\",\"authors\":\"Haoxi Chai, Yijun Ruan\",\"doi\":\"10.1016/j.tig.2025.07.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The genome is packaged with nuclear proteins and RNAs into a complex structure known as chromatin. Its dynamic organization influences genome functions and nuclear properties. Since 2009, high-throughput DNA sequencing methods such as Hi-C and ChIA-PET have pioneered genome-wide mapping of chromatin folding architectures and have given rise to the field of three-dimensional (3D) genome biology. Now, after 15 years of development, this field has experienced a remarkable growth and is still expanding rapidly. It is significantly deepening our understanding of how genome organization affects nuclear functions in various biological systems. In this review we focus on the breakthroughs and expansion of sequencing-based technologies in mapping 3D genomic landscapes and envisage the next frontiers in advancing the 3D genome biology.</p>\",\"PeriodicalId\":54413,\"journal\":{\"name\":\"Trends in Genetics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":16.3000,\"publicationDate\":\"2025-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Trends in Genetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.tig.2025.07.010\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.tig.2025.07.010","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
3D genome sequencing technology in its mid-teens: past, present, and future.
The genome is packaged with nuclear proteins and RNAs into a complex structure known as chromatin. Its dynamic organization influences genome functions and nuclear properties. Since 2009, high-throughput DNA sequencing methods such as Hi-C and ChIA-PET have pioneered genome-wide mapping of chromatin folding architectures and have given rise to the field of three-dimensional (3D) genome biology. Now, after 15 years of development, this field has experienced a remarkable growth and is still expanding rapidly. It is significantly deepening our understanding of how genome organization affects nuclear functions in various biological systems. In this review we focus on the breakthroughs and expansion of sequencing-based technologies in mapping 3D genomic landscapes and envisage the next frontiers in advancing the 3D genome biology.
期刊介绍：
Launched in 1985, Trends in Genetics swiftly established itself as a "must-read" for geneticists, offering concise, accessible articles covering a spectrum of topics from developmental biology to evolution. This reputation endures, making TiG a cherished resource in the genetic research community. While evolving with the field, the journal now embraces new areas like genomics, epigenetics, and computational genetics, alongside its continued coverage of traditional subjects such as transcriptional regulation, population genetics, and chromosome biology.
Despite expanding its scope, the core objective of TiG remains steadfast: to furnish researchers and students with high-quality, innovative reviews, commentaries, and discussions, fostering an appreciation for advances in genetic research. Each issue of TiG presents lively and up-to-date Reviews and Opinions, alongside shorter articles like Science & Society and Spotlight pieces. Invited from leading researchers, Reviews objectively chronicle recent developments, Opinions provide a forum for debate and hypothesis, and shorter articles explore the intersection of genetics with science and policy, as well as emerging ideas in the field. All articles undergo rigorous peer-review.
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