{"title":"谷胱甘肽s -转移酶Pi 1 （GSTP1）基因多态性在伊拉克乳腺癌患者中的可能关联","authors":"Hasan Alhayali, Zohreh Hojati","doi":"10.61186/rbmb.13.4.516","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Investigating the role of glutathione S-transferase pi 1 (GSTP1) in breast cancer development and exploring genetic variations in GSTP1 that may contribute to susceptibility to the disease.</p><p><strong>Methods: </strong>Blood samples were collected from 40 healthy control individuals and 75 breast cancer patients for genomic DNA extraction. PCR and bioinformatics analysis were used to examine the GSTP1 gene sequences.</p><p><strong>Results: </strong>Gel electrophoresis confirmed the presence of a 433-bp amplified genetic locus with a 212C>A variation in the intron-4 region, identified as rs757152293. The Single nucleotide polymorphisms (SNP) exhibited variable distributions, with homozygous CC and heterozygous CA genotypes. Patient samples with the CA genotype were submitted to NCBI under accession numbers OL957029-OL957036, while those with the CC genotype were submitted under accession numbers OL957027-OL957034. Additionally, sequence analysis of the rs757152293 SNP in healthy individuals was submitted to NCBI under accession numbers OL957037-OL957041.</p><p><strong>Conclusions: </strong>This study is the first to suggest a possible association between GSTP1 genetic polymorphism and breast cancer in the investigated population.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"516-524"},"PeriodicalIF":1.2000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367208/pdf/","citationCount":"0","resultStr":"{\"title\":\"Possible Association of Glutathione S-Transferase Pi 1 (GSTP1) Gene Polymorphism in Iraqi Patients with Breast Cancer.\",\"authors\":\"Hasan Alhayali, Zohreh Hojati\",\"doi\":\"10.61186/rbmb.13.4.516\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Investigating the role of glutathione S-transferase pi 1 (GSTP1) in breast cancer development and exploring genetic variations in GSTP1 that may contribute to susceptibility to the disease.</p><p><strong>Methods: </strong>Blood samples were collected from 40 healthy control individuals and 75 breast cancer patients for genomic DNA extraction. PCR and bioinformatics analysis were used to examine the GSTP1 gene sequences.</p><p><strong>Results: </strong>Gel electrophoresis confirmed the presence of a 433-bp amplified genetic locus with a 212C>A variation in the intron-4 region, identified as rs757152293. The Single nucleotide polymorphisms (SNP) exhibited variable distributions, with homozygous CC and heterozygous CA genotypes. Patient samples with the CA genotype were submitted to NCBI under accession numbers OL957029-OL957036, while those with the CC genotype were submitted under accession numbers OL957027-OL957034. Additionally, sequence analysis of the rs757152293 SNP in healthy individuals was submitted to NCBI under accession numbers OL957037-OL957041.</p><p><strong>Conclusions: </strong>This study is the first to suggest a possible association between GSTP1 genetic polymorphism and breast cancer in the investigated population.</p>\",\"PeriodicalId\":45319,\"journal\":{\"name\":\"Reports of Biochemistry and Molecular Biology\",\"volume\":\"13 4\",\"pages\":\"516-524\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367208/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reports of Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.61186/rbmb.13.4.516\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reports of Biochemistry and Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.61186/rbmb.13.4.516","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Possible Association of Glutathione S-Transferase Pi 1 (GSTP1) Gene Polymorphism in Iraqi Patients with Breast Cancer.
Background: Investigating the role of glutathione S-transferase pi 1 (GSTP1) in breast cancer development and exploring genetic variations in GSTP1 that may contribute to susceptibility to the disease.
Methods: Blood samples were collected from 40 healthy control individuals and 75 breast cancer patients for genomic DNA extraction. PCR and bioinformatics analysis were used to examine the GSTP1 gene sequences.
Results: Gel electrophoresis confirmed the presence of a 433-bp amplified genetic locus with a 212C>A variation in the intron-4 region, identified as rs757152293. The Single nucleotide polymorphisms (SNP) exhibited variable distributions, with homozygous CC and heterozygous CA genotypes. Patient samples with the CA genotype were submitted to NCBI under accession numbers OL957029-OL957036, while those with the CC genotype were submitted under accession numbers OL957027-OL957034. Additionally, sequence analysis of the rs757152293 SNP in healthy individuals was submitted to NCBI under accession numbers OL957037-OL957041.
Conclusions: This study is the first to suggest a possible association between GSTP1 genetic polymorphism and breast cancer in the investigated population.
期刊介绍：
The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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