利用傅立叶变换红外光谱技术通过宫颈拭子无创诊断子宫内膜瘤。

IF 1.3 Q4 OBSTETRICS & GYNECOLOGY
Aslı Karakaşlı, Ümit Görkem, Cihan Toğrul, Engin Yıldırım, Dursun Ali Köse, Ömer Yurdakul
{"title":"利用傅立叶变换红外光谱技术通过宫颈拭子无创诊断子宫内膜瘤。","authors":"Aslı Karakaşlı, Ümit Görkem, Cihan Toğrul, Engin Yıldırım, Dursun Ali Köse, Ömer Yurdakul","doi":"10.4274/tjod.galenos.2025.26980","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate whether Fourier transform infrared (FTIR) spectroscopy applied to cervical swab samples could detect meaningful biochemical differences between women diagnosed with endometriomas and healthy controls, thereby assessing its potential as a non-invasive diagnostic tool.</p><p><strong>Materials and methods: </strong>A total of 104 cervical swab samples-52 from women with endometriomas diagnosed via transvaginal ultrasonography and 52 from healthy controls-were initially collected and processed. Following an optimization process and quality control of spectral data, 24 endometrioma and 20 control samples were included in the final analysis. FTIR spectra were obtained in the 4000-600 cm<sup>-1</sup> range, and the primary outcomes included comparative peak intensities and areas under specific wavenumbers reflecting various bio-organic molecules.</p><p><strong>Results: </strong>Statistically significant differences were observed at 2350 cm<sup>-1</sup> and 1050 cm<sup>-1</sup>, indicative of alterations in carbon dioxide and carbohydrate metabolism, respectively, in the endometrioma group compared with healthy controls (p<0.05). No significant differences were detected in other spectral regions associated with lipids (2950, 1460, 1400 cm<sup>-1</sup>) and proteins (e.g., amid-I and amid-II regions), suggesting that endometrioma may primarily affect carbohydrate metabolism and carbon dioxide balance rather than lipid and protein pathways. Both groups were comparable in demographic and hormonal characteristics, thus bolstering the validity of the findings.</p><p><strong>Conclusion: </strong>FTIR spectroscopy of cervical swab samples revealed distinctive biochemical profiles in women with endometriomas, particularly related to carbon dioxide and carbohydrate metabolism. These data suggest that FTIR analysis, which is rapid and minimally invasive, holds promise for the future development of non-invasive diagnostic strategies for endometrioma. However, larger multicenter studies that include surgical confirmation and disease staging are needed to establish its clinical utility definitively.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":" ","pages":"186-193"},"PeriodicalIF":1.3000,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411982/pdf/","citationCount":"0","resultStr":"{\"title\":\"Non-invasive diagnosis of endometrioma through cervical swabs using Fourier transform infrared spectroscopy.\",\"authors\":\"Aslı Karakaşlı, Ümit Görkem, Cihan Toğrul, Engin Yıldırım, Dursun Ali Köse, Ömer Yurdakul\",\"doi\":\"10.4274/tjod.galenos.2025.26980\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aimed to investigate whether Fourier transform infrared (FTIR) spectroscopy applied to cervical swab samples could detect meaningful biochemical differences between women diagnosed with endometriomas and healthy controls, thereby assessing its potential as a non-invasive diagnostic tool.</p><p><strong>Materials and methods: </strong>A total of 104 cervical swab samples-52 from women with endometriomas diagnosed via transvaginal ultrasonography and 52 from healthy controls-were initially collected and processed. Following an optimization process and quality control of spectral data, 24 endometrioma and 20 control samples were included in the final analysis. FTIR spectra were obtained in the 4000-600 cm<sup>-1</sup> range, and the primary outcomes included comparative peak intensities and areas under specific wavenumbers reflecting various bio-organic molecules.</p><p><strong>Results: </strong>Statistically significant differences were observed at 2350 cm<sup>-1</sup> and 1050 cm<sup>-1</sup>, indicative of alterations in carbon dioxide and carbohydrate metabolism, respectively, in the endometrioma group compared with healthy controls (p<0.05). No significant differences were detected in other spectral regions associated with lipids (2950, 1460, 1400 cm<sup>-1</sup>) and proteins (e.g., amid-I and amid-II regions), suggesting that endometrioma may primarily affect carbohydrate metabolism and carbon dioxide balance rather than lipid and protein pathways. Both groups were comparable in demographic and hormonal characteristics, thus bolstering the validity of the findings.</p><p><strong>Conclusion: </strong>FTIR spectroscopy of cervical swab samples revealed distinctive biochemical profiles in women with endometriomas, particularly related to carbon dioxide and carbohydrate metabolism. These data suggest that FTIR analysis, which is rapid and minimally invasive, holds promise for the future development of non-invasive diagnostic strategies for endometrioma. However, larger multicenter studies that include surgical confirmation and disease staging are needed to establish its clinical utility definitively.</p>\",\"PeriodicalId\":45340,\"journal\":{\"name\":\"Turkish Journal of Obstetrics and Gynecology\",\"volume\":\" \",\"pages\":\"186-193\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411982/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkish Journal of Obstetrics and Gynecology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4274/tjod.galenos.2025.26980\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/9/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Obstetrics and Gynecology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4274/tjod.galenos.2025.26980","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/2 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目的:本研究旨在探讨傅立叶变换红外(FTIR)光谱技术应用于宫颈拭子样本是否能检测出诊断为子宫内膜异位瘤的女性与健康对照组之间有意义的生化差异,从而评估其作为无创诊断工具的潜力。材料和方法:最初收集并处理了104份宫颈拭子样本,其中52份来自经阴道超声诊断为子宫内膜异位瘤的女性,52份来自健康对照组。经过光谱数据的优化过程和质量控制,24例子宫内膜瘤和20例对照样本被纳入最终分析。在4000-600 cm-1范围内获得了FTIR光谱,主要结果包括反映各种生物有机分子的比较峰强度和特定波数下的面积。结果:在2350 cm-1和1050 cm-1处观察到具有统计学意义的差异,这分别表明子宫内膜异位瘤组与健康对照组(p-1)和蛋白质(例如,mid- i和mid- ii区域)的二氧化碳和碳水化合物代谢发生了变化,这表明子宫内膜异位瘤可能主要影响碳水化合物代谢和二氧化碳平衡,而不是脂质和蛋白质途径。两组在人口统计学和激素特征上具有可比性,从而增强了研究结果的有效性。结论:FTIR光谱显示子宫内膜异位瘤妇女的独特生化特征,特别是与二氧化碳和碳水化合物代谢有关。这些数据表明,快速、微创的FTIR分析为子宫内膜异位瘤的无创诊断策略的未来发展提供了希望。然而,需要更大规模的多中心研究,包括手术确认和疾病分期,以确定其临床应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Non-invasive diagnosis of endometrioma through cervical swabs using Fourier transform infrared spectroscopy.

Non-invasive diagnosis of endometrioma through cervical swabs using Fourier transform infrared spectroscopy.

Non-invasive diagnosis of endometrioma through cervical swabs using Fourier transform infrared spectroscopy.

Objective: This study aimed to investigate whether Fourier transform infrared (FTIR) spectroscopy applied to cervical swab samples could detect meaningful biochemical differences between women diagnosed with endometriomas and healthy controls, thereby assessing its potential as a non-invasive diagnostic tool.

Materials and methods: A total of 104 cervical swab samples-52 from women with endometriomas diagnosed via transvaginal ultrasonography and 52 from healthy controls-were initially collected and processed. Following an optimization process and quality control of spectral data, 24 endometrioma and 20 control samples were included in the final analysis. FTIR spectra were obtained in the 4000-600 cm-1 range, and the primary outcomes included comparative peak intensities and areas under specific wavenumbers reflecting various bio-organic molecules.

Results: Statistically significant differences were observed at 2350 cm-1 and 1050 cm-1, indicative of alterations in carbon dioxide and carbohydrate metabolism, respectively, in the endometrioma group compared with healthy controls (p<0.05). No significant differences were detected in other spectral regions associated with lipids (2950, 1460, 1400 cm-1) and proteins (e.g., amid-I and amid-II regions), suggesting that endometrioma may primarily affect carbohydrate metabolism and carbon dioxide balance rather than lipid and protein pathways. Both groups were comparable in demographic and hormonal characteristics, thus bolstering the validity of the findings.

Conclusion: FTIR spectroscopy of cervical swab samples revealed distinctive biochemical profiles in women with endometriomas, particularly related to carbon dioxide and carbohydrate metabolism. These data suggest that FTIR analysis, which is rapid and minimally invasive, holds promise for the future development of non-invasive diagnostic strategies for endometrioma. However, larger multicenter studies that include surgical confirmation and disease staging are needed to establish its clinical utility definitively.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.10
自引率
0.00%
发文量
1
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信