果蝇伤害感受器对损伤翻译反应的RNA测序数据集。

IF 2 Q3 COMPUTER SCIENCE, INFORMATION SYSTEMS

Data Pub Date : 2025-02-01 Epub Date: 2025-01-21 DOI:10.3390/data10020011

Christine M Hale, Kyle J Beauchemin, Courtney L Brann, Julie K Moulton, Ramaz Geguchadze, Benjamin J Harrison, Geoffrey K Ganter

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引用次数: 0

摘要

为了研究损伤诱导的伤害感受器致敏机制，我们对受伤果蝇幼虫和未受伤果蝇幼虫的伤害感受器的翻译体进行了测序。通过GAL4/UAS方法，在第4类树突状树突神经元中表达绿色荧光蛋白（GFP）标记的核糖体亚基的3龄幼虫被紫外线或假损伤，这些幼虫被认为是表达偷窃的初级伤害受体。对幼虫进行GFP标签的翻译核糖体亲和纯化，并对伤害受体特异性核糖体结合RNA进行测序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

RNA Sequencing Dataset of Drosophila Nociceptor Translatomic Response to Injury.

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RNA Sequencing Dataset of Drosophila Nociceptor Translatomic Response to Injury.

To prepare to address the mechanisms of injury-induced nociceptor sensitization, we sequenced the translatome of the nociceptors of injured Drososophila larvae and those of uninjured larvae. Third-instar larvae expressing a green fluorescent protein (GFP)-tagged ribosomal subunit specifically in Class 4 dendritic arborization neurons, recognized as pickpocket-expressing primary nociceptors, via the GAL4/UAS method, were injured by ultraviolet light or sham-injured. Larvae were subjected to translating ribosome affinity purification for the GFP tag and nociceptor-specific ribosome-bound RNA was sequenced.

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来源期刊

Data Decision Sciences-Information Systems and Management

CiteScore

4.30

自引率

3.80%

发文量

审稿时长

10 weeks