{"title":"ClPS1基因介导的2n花粉形成调控可以产生三倍体无籽西瓜。","authors":"Wenyu Pang, Qiaran Wang, Chenxin Li, Wenbing He, Jiafa Wang, Shujuan Tian, Li Yuan","doi":"10.1186/s43897-025-00170-2","DOIUrl":null,"url":null,"abstract":"<p><p>Seedless watermelons are increasingly dominating the consumer market due to their convenience and high quality. However, traditional triploid watermelon breeding faces challenges such as long breeding cycles and low survival rates of triploid F<sub>1</sub> progeny, severely hindering both breeding and production. In this study, we identified the ClPS1 gene as being associated with the formation of 2n gametes in watermelon. Expression analysis revealed that ClPS1 is highly expressed during meiosis and microsporogenesis. Using CRISPR/Cas9, we generated ClPS1-targeted mutants, which disrupted chromosome segregation at metaphase II. This led to the production of diploid male spores and abnormal division of male spores, ultimately generating diploid pollen grains, while female meiosis remained unaffected. Moreover, self-fertilization or crosses using these mutants as paternal parents yielded triploid and aneuploid watermelons. Our findings demonstrate, for the first time, the molecular manipulation of 2n gametes to create triploid seedless watermelons, offering new insights into polyploid breeding and evolutionary studies in the Cucurbitaceae family and other species.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"48"},"PeriodicalIF":8.1000,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403409/pdf/","citationCount":"0","resultStr":"{\"title\":\"ClPS1 gene-mediated manipulation of 2n pollen formation enables the creation of triploid seedless watermelon.\",\"authors\":\"Wenyu Pang, Qiaran Wang, Chenxin Li, Wenbing He, Jiafa Wang, Shujuan Tian, Li Yuan\",\"doi\":\"10.1186/s43897-025-00170-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Seedless watermelons are increasingly dominating the consumer market due to their convenience and high quality. However, traditional triploid watermelon breeding faces challenges such as long breeding cycles and low survival rates of triploid F<sub>1</sub> progeny, severely hindering both breeding and production. In this study, we identified the ClPS1 gene as being associated with the formation of 2n gametes in watermelon. Expression analysis revealed that ClPS1 is highly expressed during meiosis and microsporogenesis. Using CRISPR/Cas9, we generated ClPS1-targeted mutants, which disrupted chromosome segregation at metaphase II. This led to the production of diploid male spores and abnormal division of male spores, ultimately generating diploid pollen grains, while female meiosis remained unaffected. Moreover, self-fertilization or crosses using these mutants as paternal parents yielded triploid and aneuploid watermelons. Our findings demonstrate, for the first time, the molecular manipulation of 2n gametes to create triploid seedless watermelons, offering new insights into polyploid breeding and evolutionary studies in the Cucurbitaceae family and other species.</p>\",\"PeriodicalId\":29970,\"journal\":{\"name\":\"Molecular Horticulture\",\"volume\":\"5 1\",\"pages\":\"48\"},\"PeriodicalIF\":8.1000,\"publicationDate\":\"2025-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403409/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Horticulture\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s43897-025-00170-2\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Horticulture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s43897-025-00170-2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
ClPS1 gene-mediated manipulation of 2n pollen formation enables the creation of triploid seedless watermelon.
Seedless watermelons are increasingly dominating the consumer market due to their convenience and high quality. However, traditional triploid watermelon breeding faces challenges such as long breeding cycles and low survival rates of triploid F1 progeny, severely hindering both breeding and production. In this study, we identified the ClPS1 gene as being associated with the formation of 2n gametes in watermelon. Expression analysis revealed that ClPS1 is highly expressed during meiosis and microsporogenesis. Using CRISPR/Cas9, we generated ClPS1-targeted mutants, which disrupted chromosome segregation at metaphase II. This led to the production of diploid male spores and abnormal division of male spores, ultimately generating diploid pollen grains, while female meiosis remained unaffected. Moreover, self-fertilization or crosses using these mutants as paternal parents yielded triploid and aneuploid watermelons. Our findings demonstrate, for the first time, the molecular manipulation of 2n gametes to create triploid seedless watermelons, offering new insights into polyploid breeding and evolutionary studies in the Cucurbitaceae family and other species.
期刊介绍:
Aims
Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field.
Scope
Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants):
▪ Developmental and evolutionary biology
▪ Physiology, biochemistry and cell biology
▪ Plant-microbe and plant-environment interactions
▪ Genetics and epigenetics
▪ Molecular breeding and biotechnology
▪ Secondary metabolism and synthetic biology
▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome.
The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest.
In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.