牙周蛋白在牙滤泡破骨细胞分化中的作用机制分析:附2例报告。

IF 1 4区 医学 Q3 MEDICINE, GENERAL & INTERNAL
Jun Cai, Han Qin
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引用次数: 0

摘要

背景:本研究对2例牙齿萌牙障碍患者的牙滤泡及牙槽骨进行分析,旨在从细胞外基质对牙滤泡破骨细胞分化的影响角度,为探讨该病的病因及选择治疗方案提供一定的参考。病例总结:分别采集1例单牙萌牙障碍患者和1例全恒牙萌牙障碍患者的牙囊和牙槽骨组织。同时采集因正畸治疗需要拔除的阻塞牙的牙囊和牙槽骨组织作为对照组。采用苏木精和伊红(HE)染色观察牙滤泡细胞形态。采用免疫组化染色法观察牙滤泡和牙槽骨组织中骨膜蛋白、核因子κ B受体激活物配体(RANKL)、骨保护素(OPG)蛋白的表达。并观察恒牙胚芽除去牙冠阻力后的出牙情况。结论:2例牙滤泡组织的HE染色显示,牙滤泡细胞体积减小,细胞核浓缩,细胞纤维化。免疫组化染色显示,牙滤泡和牙槽骨组织中骨膜蛋白表达升高,RANKL和OPG蛋白表达降低,RANKL/OPG比值降低。除去阻力后,恒牙胚芽通常表现为正常的萌出。出牙障碍可能伴随着牙周蛋白的异常重塑,影响牙滤泡内破骨细胞的分化功能,导致牙槽骨代谢失衡,导致出牙障碍。无论是单牙还是全恒牙萌牙障碍,一旦去除冠状阻力,牙齿往往都能正常萌牙。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanism analysis of periostin in osteoclasts differentiation of dental follicle: Two case reports.

Background: This study analyzed the dental follicle and alveolar bone of two patients with tooth eruption disorders, aiming to provide some reference for exploring the etiology and selecting treatment plans of this disease from the perspective of the influence of extracellular matrix on osteoclasts differentiation in dental follicle.

Case summary: Collect dental follicle and alveolar bone tissue from one patient with single tooth eruption disorder and one patient with full permanent tooth eruption disorder, respectively. Simultaneously collect the dental follicle and alveolar bone tissue of obstructed teeth that need to be extracted due to orthodontic treatment as the control group. Hematoxylin and eosin (HE) staining was used to observe the morphology of dental follicle cells. Immunohistochemical staining was used to observe the expression of periostin, receptor activator of nuclear factor kappa B ligand (RANKL), and osteoprotegerin (OPG) protein in dental follicle and alveolar bone tissue. And observe the eruption of teeth after removing resistance from the crown of the permanent tooth germ.

Conclusion: HE staining of two cases of dental follicle tissues showed that the volume of dental follicle cells decreased, the nuclei were condensed, and there seemed to be cellular fibrosis. The immunohistochemical staining showed that both the dental follicle and alveolar bone tissue exhibited increased expression of periostin, decreased expression of RANKL and OPG proteins, and decreased RANKL/OPG ratio. After removing resistance, the permanent tooth germ often appears to have normal eruption. Tooth eruption disorders may be accompanied by abnormal remodeling of periostin, which affects the differentiation function of osteoclasts in the dental follicle and leads to metabolic imbalance of alveolar bone, resulting in tooth eruption disorders. Whether it is a single or full permanent tooth eruption disorder, once the coronal resistance is removed, the teeth can often erupt normally.

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来源期刊
World Journal of Clinical Cases
World Journal of Clinical Cases Medicine-General Medicine
自引率
0.00%
发文量
3384
期刊介绍: The World Journal of Clinical Cases (WJCC) is a high-quality, peer reviewed, open-access journal. The primary task of WJCC is to rapidly publish high-quality original articles, reviews, editorials, and case reports in the field of clinical cases. In order to promote productive academic communication, the peer review process for the WJCC is transparent; to this end, all published manuscripts are accompanied by the anonymized reviewers’ comments as well as the authors’ responses. The primary aims of the WJCC are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in clinical cases.
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