{"title":"糖尿病视网膜病变患者房水中总抗氧化能力和抗坏血酸水平的分析。","authors":"Yen-Ning Chen, Yi-Jen Hsueh, Chao-Kai Chang, Po-Yi Wu, Yu-Ting Tsao, Yi-Hsing Chen, Eugene Yu-Chuan Kang, Chao-Min Cheng, Wei-Chi Wu, Yih-Shiou Hwang, Hung-Chi Chen","doi":"10.1167/tvst.14.8.31","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to investigate aqueous humor total antioxidant capacity (TAC) and ascorbic acid (AA) levels of patients with diabetic retinopathy (DR), and their correlation with serum glycated hemoglobin (HbA1c) levels.</p><p><strong>Methods: </strong>This case-control study enrolled 177 eyes from 177 patients that underwent cataract surgery between April 2019 and November 2022 in Chang Gung Memorial Hospital. Group 1 comprised 103 eyes from patients without diabetes; group 2 comprised 40 eyes from patients with diabetes but without DR; and group 3 comprised 34 eyes from patients with DR. Aqueous humor samples were collected and analyzed for TAC and AA levels using the ferric reducing/antioxidant AA assay.</p><p><strong>Results: </strong>Aqueous humor TAC and AA levels were decreased in group 3 compared with group 2 and group 1 (P < 0.001 and P < 0.001). Aqueous humor TAC and AA levels were independent protective factors against DR in the diabetes population, with adjusted odds ratios of 0.109 (95% confidence interval [CI] = 0.017-0.719, P = 0.021) and 0.095 (95% CI = 0.012-0.726, P = 0.023), respectively. Aqueous humor TAC and AA concentrations were weakly negatively correlated with serum HbA1c levels, with Pearson's correlation coefficients of -0.339 (P = 0.013) and -0.341 (P = 0.012), respectively.</p><p><strong>Conclusions: </strong>TAC and AA had protective effects against DR. Decreased aqueous humor TAC and AA levels may serve as potential biomarkers for DR among the diabetes population.</p><p><strong>Translational relevance: </strong>This study highlights the role of antioxidants in DR and provides foundation for future research on AA supplementation's potential for the prophylaxis and treatment of DR.</p>","PeriodicalId":23322,"journal":{"name":"Translational Vision Science & Technology","volume":"14 8","pages":"31"},"PeriodicalIF":2.6000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372942/pdf/","citationCount":"0","resultStr":"{\"title\":\"Analysis of Total Antioxidant Capacity and Ascorbic Acid Levels in the Aqueous Humor of Patients With Diabetic Retinopathy.\",\"authors\":\"Yen-Ning Chen, Yi-Jen Hsueh, Chao-Kai Chang, Po-Yi Wu, Yu-Ting Tsao, Yi-Hsing Chen, Eugene Yu-Chuan Kang, Chao-Min Cheng, Wei-Chi Wu, Yih-Shiou Hwang, Hung-Chi Chen\",\"doi\":\"10.1167/tvst.14.8.31\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>The purpose of this study was to investigate aqueous humor total antioxidant capacity (TAC) and ascorbic acid (AA) levels of patients with diabetic retinopathy (DR), and their correlation with serum glycated hemoglobin (HbA1c) levels.</p><p><strong>Methods: </strong>This case-control study enrolled 177 eyes from 177 patients that underwent cataract surgery between April 2019 and November 2022 in Chang Gung Memorial Hospital. Group 1 comprised 103 eyes from patients without diabetes; group 2 comprised 40 eyes from patients with diabetes but without DR; and group 3 comprised 34 eyes from patients with DR. Aqueous humor samples were collected and analyzed for TAC and AA levels using the ferric reducing/antioxidant AA assay.</p><p><strong>Results: </strong>Aqueous humor TAC and AA levels were decreased in group 3 compared with group 2 and group 1 (P < 0.001 and P < 0.001). Aqueous humor TAC and AA levels were independent protective factors against DR in the diabetes population, with adjusted odds ratios of 0.109 (95% confidence interval [CI] = 0.017-0.719, P = 0.021) and 0.095 (95% CI = 0.012-0.726, P = 0.023), respectively. Aqueous humor TAC and AA concentrations were weakly negatively correlated with serum HbA1c levels, with Pearson's correlation coefficients of -0.339 (P = 0.013) and -0.341 (P = 0.012), respectively.</p><p><strong>Conclusions: </strong>TAC and AA had protective effects against DR. Decreased aqueous humor TAC and AA levels may serve as potential biomarkers for DR among the diabetes population.</p><p><strong>Translational relevance: </strong>This study highlights the role of antioxidants in DR and provides foundation for future research on AA supplementation's potential for the prophylaxis and treatment of DR.</p>\",\"PeriodicalId\":23322,\"journal\":{\"name\":\"Translational Vision Science & Technology\",\"volume\":\"14 8\",\"pages\":\"31\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372942/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Translational Vision Science & Technology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1167/tvst.14.8.31\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OPHTHALMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Translational Vision Science & Technology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1167/tvst.14.8.31","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的：研究糖尿病视网膜病变（DR）患者房水总抗氧化能力（TAC）和抗坏血酸（AA）水平及其与血清糖化血红蛋白（HbA1c）水平的相关性。方法：本病例对照研究纳入了2019年4月至2022年11月在长庚纪念医院接受白内障手术的177例患者的177只眼睛。第一组为非糖尿病患者103只眼；第二组40只眼来自糖尿病患者，但无DR；第3组包括34只dr患者的眼睛，收集房水样品并使用铁还原/抗氧化AA法分析TAC和AA水平。结果：3组患者房水TAC、AA水平明显低于2、1组（P < 0.001、P < 0.001）。房水中TAC和AA水平是糖尿病人群发生DR的独立保护因素，校正后的比值比分别为0.109（95%可信区间[CI] = 0.017-0.719, P = 0.021）和0.095 （95% CI = 0.012-0.726, P = 0.023）。房水TAC和AA浓度与血清HbA1c水平呈弱负相关，Pearson相关系数分别为-0.339 （P = 0.013）和-0.341 （P = 0.012）。结论：TAC和AA对DR有保护作用，房水中TAC和AA水平降低可能是糖尿病人群DR的潜在生物标志物。翻译相关性：本研究突出了抗氧化剂在DR中的作用，为进一步研究补充AA对DR的预防和治疗潜力奠定了基础。
Analysis of Total Antioxidant Capacity and Ascorbic Acid Levels in the Aqueous Humor of Patients With Diabetic Retinopathy.
Purpose: The purpose of this study was to investigate aqueous humor total antioxidant capacity (TAC) and ascorbic acid (AA) levels of patients with diabetic retinopathy (DR), and their correlation with serum glycated hemoglobin (HbA1c) levels.
Methods: This case-control study enrolled 177 eyes from 177 patients that underwent cataract surgery between April 2019 and November 2022 in Chang Gung Memorial Hospital. Group 1 comprised 103 eyes from patients without diabetes; group 2 comprised 40 eyes from patients with diabetes but without DR; and group 3 comprised 34 eyes from patients with DR. Aqueous humor samples were collected and analyzed for TAC and AA levels using the ferric reducing/antioxidant AA assay.
Results: Aqueous humor TAC and AA levels were decreased in group 3 compared with group 2 and group 1 (P < 0.001 and P < 0.001). Aqueous humor TAC and AA levels were independent protective factors against DR in the diabetes population, with adjusted odds ratios of 0.109 (95% confidence interval [CI] = 0.017-0.719, P = 0.021) and 0.095 (95% CI = 0.012-0.726, P = 0.023), respectively. Aqueous humor TAC and AA concentrations were weakly negatively correlated with serum HbA1c levels, with Pearson's correlation coefficients of -0.339 (P = 0.013) and -0.341 (P = 0.012), respectively.
Conclusions: TAC and AA had protective effects against DR. Decreased aqueous humor TAC and AA levels may serve as potential biomarkers for DR among the diabetes population.
Translational relevance: This study highlights the role of antioxidants in DR and provides foundation for future research on AA supplementation's potential for the prophylaxis and treatment of DR.
期刊介绍：
Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO.
The journal covers a broad spectrum of work, including but not limited to:
Applications of stem cell technology for regenerative medicine,
Development of new animal models of human diseases,
Tissue bioengineering,
Chemical engineering to improve virus-based gene delivery,
Nanotechnology for drug delivery,
Design and synthesis of artificial extracellular matrices,
Development of a true microsurgical operating environment,
Refining data analysis algorithms to improve in vivo imaging technology,
Results of Phase 1 clinical trials,
Reverse translational ("bedside to bench") research.
TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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