通过高级预处理提高间充质干细胞细胞外囊泡对神经退行性疾病的治疗潜力。

IF 3.3 3区 医学 Q2 CELL & TISSUE ENGINEERING
Stem Cells International Pub Date : 2025-08-21 eCollection Date: 2025-01-01 DOI:10.1155/sci/2616653
Cristina D'Arrigo, Sara Labbate, Denise Galante
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引用次数: 0

摘要

急性和慢性神经退行性疾病(NDs)是全世界致残和死亡的主要原因。急性NDs包括中风、创伤性脑损伤(TBI)和脊髓损伤(SCI)等病症。另一方面,慢性NDs包括阿尔茨海默病(AD)、帕金森病(PD)、亨廷顿病(HD)、多发性硬化症(MS)和肌萎缩侧索硬化症(ALS)。目前,这些疾病没有明确的治疗方法,现有的治疗方法主要集中在减缓症状的进展。间充质干细胞(Mesenchymal stem cells, MSCs)因其多系分化能力、免疫调节能力和再生特性而受到再生医学的关注。近年来,来自间充质干细胞的细胞外囊泡(EVs)作为一种无细胞治疗方法显示出巨大的前景,消除了直接使用间充质干细胞相关的风险,如移植后的致瘤性和细胞存活率差。电动汽车已成为细胞间通讯和组织修复的强大介质,具有免疫调节、抗炎和促再生特性。然而,由于骨髓间充质干细胞的复制性衰老导致的EVs产量低和疗效降低等局限性限制了它们的治疗潜力。预处理策略,包括缺氧、3D培养和生化启动,已经在其他领域进行了探索,以提高电动汽车的性能,但它们在nd中的具体应用仍然缺乏报道。本综述旨在通过分析用于提高间充质干细胞衍生的内皮细胞治疗神经退行性疾病潜力的预处理方法来解决这一空白。这些预处理策略可以提高电动汽车的产量、功能货量和治疗急慢性NDs的靶向治疗效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Boosting the Therapeutic Potential of Extracellular Vesicles Derived From Mesenchymal Stem Cells via Advanced Preconditioning for Neurodegenerative Disorders.

Boosting the Therapeutic Potential of Extracellular Vesicles Derived From Mesenchymal Stem Cells via Advanced Preconditioning for Neurodegenerative Disorders.

Boosting the Therapeutic Potential of Extracellular Vesicles Derived From Mesenchymal Stem Cells via Advanced Preconditioning for Neurodegenerative Disorders.

Acute and chronic neurodegenerative conditions (NDs) are major causes of disability and mortality worldwide. Acute NDs encompass conditions such as stroke, traumatic brain injury (TBI), and spinal cord injury (SCI). On the other hand, chronic NDs include Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), multiple sclerosis (MS), and amyotrophic lateral sclerosis (ALS). Currently, no definitive cure exists for these diseases, and available therapies focus primarily on slowing the progression of symptoms. Mesenchymal stem cells (MSCs), due to their multilineage differentiation capacity, immunomodulatory abilities, and regenerative properties, have gained attention in regenerative medicine. In recent years, extracellular vesicles (EVs) derived from MSCs have shown great promise as a cell-free therapeutic approach, eliminating the risks associated with direct MSCs use, such as tumorigenicity and poor cell survival after transplantation. EVs have emerged as powerful mediators of intercellular communication and tissue repair, exhibiting immunomodulatory, anti-inflammatory, and proregenerative properties. However, limitations such as low EVs yield and reduced efficacy due to MSCs replicative senescence restrict their therapeutic potential. Preconditioning strategies, including hypoxia, 3D cultures, and biochemical priming, have been explored in other fields to enhance EVs properties, yet their specific application to NDs remains under-reported. This review aims to address this gap by analyzing the preconditioning methods used to boost the therapeutic potential of MSCs-derived EVs for neurodegenerative diseases. These preconditioning strategies may enhance EVs yield, functional cargo, and targeted therapeutic efficacy for treating acute and chronic NDs.

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来源期刊
Stem Cells International
Stem Cells International CELL & TISSUE ENGINEERING-
CiteScore
8.10
自引率
2.30%
发文量
188
审稿时长
18 weeks
期刊介绍: Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials. Topics covered include, but are not limited to: embryonic stem cells; induced pluripotent stem cells; tissue-specific stem cells; stem cell differentiation; genetics and epigenetics; cancer stem cells; stem cell technologies; ethical, legal, and social issues.
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