利用人牙髓干细胞制造生物三维导管再生免疫缺陷大鼠面神经。

IF 3.3 3区 医学 Q2 CELL & TISSUE ENGINEERING
Stem Cells International Pub Date : 2025-08-25 eCollection Date: 2025-01-01 DOI:10.1155/sci/1923945
Yuri Matsui-Chujo, Ayano Hatori, Monika Nakano, Yuki Kanno, Ryosuke Ikeguchi, Tomoki Aoyama, Kazuaki Fujita, Yudai Miyazaki, Yoko Torii, Shizuka Akieda, Daichi Chikazu, Yoko Kawase-Koga
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引用次数: 0

摘要

颌面部肿瘤手术或外伤可损伤周围神经,如面神经。自体神经移植是治疗周围神经损伤的金标准。由于需要新的周围神经再生技术,使用生物3D打印机仅用细胞制造三维(3D)结构引起了人们的关注。牙髓干细胞(DPSCs)是一种很有前途的细胞来源,因为它们具有高克隆性、增殖性和多分化潜力。在本研究中,利用生物3D打印机将DPSCs制成神经导管,并在大鼠面神经损伤模型中评估其神经再生潜力。从患者智齿中获得DPSCs并进行培养。利用生物3D打印机制作了一个5毫米的生物3D导管。选取10周龄、体重190 ~ 240 g的F344 /rnu-大鼠6只,分为Bio 3D组(n = 3)和硅胶管组(n = 3)。将5 mm的Bio 3D导管和硅胶管移植到4 mm的缺陷中。术后12周进行评估。两组免疫缺陷大鼠的胡须都在移动。生物3D组有髓鞘轴突数量明显大于硅胶组。生物3D组再生轴突有髓鞘直径(MAD)和髓鞘厚度(MT)显著大于硅胶组(MAD: p < 0.01, MT: p < 0.05)。在这项研究中,我们证实了由DPSCs制成的生物3D结构移植到大鼠面神经损伤模型中的神经再生潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Facial Nerve Regeneration in Immunodeficient Rats Using a Bio 3D Conduit Fabricated From Human Dental Pulp Stem Cells.

Tumor surgery or trauma in the maxillofacial region may cause injuries to peripheral nerves, such as facial nerves. The gold standard of treatment for peripheral nerve injury has been autologous nerve grafting. Since new peripheral nerve regeneration technologies are required, three-dimensional (3D) structures fabricated only from cells by using Bio 3D printers are attracting attention. Dental pulp stem cells (DPSCs) are a promising option as a cell source because of their high clonogenic, proliferative, and multidifferentiation potentials. In this study, nerve conduits were fabricated from DPSCs using a Bio 3D printer, and their potential for nerve regeneration was evaluated in a rat facial nerve injury model. DPSCs were obtained from wisdomteeth of patients and cultured. A 5 mm Bio 3D conduit was fabricated by using a Bio 3D printer. Six F344 rnu-/rnu- rats with immune deficiency (10 weeks old, body weight: 190-240 g) were divided into two groups: a Bio 3D group (n = 3) and a silicone tube group (n = 3). The 5 mm Bio 3D conduits and silicone tubes were transplanted into 4 mm defects. Evaluation was performed at 12 weeks after the surgery. The whiskers of immunodeficient rats in both groups were moving. The number of myelinated axons was larger in the Bio 3D group than in the silicone group. Myelinated axon diameter (MAD) and myelin thickness (MT) of regenerated axons in the Bio 3D group were significantly greater than those in the silicone group (MAD: p  < 0.01, MT: p  < 0.05). In this study, we confirmed the nerve regeneration potential of Bio 3D structures fabricated from DPSCs that were transplanted into a rat model of facial nerve injury.

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来源期刊
Stem Cells International
Stem Cells International CELL & TISSUE ENGINEERING-
CiteScore
8.10
自引率
2.30%
发文量
188
审稿时长
18 weeks
期刊介绍: Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials. Topics covered include, but are not limited to: embryonic stem cells; induced pluripotent stem cells; tissue-specific stem cells; stem cell differentiation; genetics and epigenetics; cancer stem cells; stem cell technologies; ethical, legal, and social issues.
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