{"title":"透射电镜观察Or基因对Or基因渗入的热带花椰菜叶绿体形态和β-胡萝卜素积累的影响。","authors":"Anamika Chandel, Shrawan Singh","doi":"10.1007/s00709-025-02106-5","DOIUrl":null,"url":null,"abstract":"<p><p>Cauliflower Or gene governs massive accumulation of β-carotene in the edible 'curd' portion. However, homozygous dominant (OrOr) plants accumulate higher β-carotene than heterozygous (Oror) individuals, yet this phenomenon has not been thoroughly elucidated in relation to chromoplast morphology. A study was performed in a complete randomised block design with three replicates, in which curd samples of homozygous orange (OrOr; CF<sub>Or-HM</sub>)<sub>,</sub> heterozygous orange (Oror; CF<sub>Or-HT</sub>) and white (oror; CF<sub>WT</sub>) genotypes were analysed using transmission electron microscopy (TEM). The number of chromoplasts in a cell and their morphology (shape and size) exhibited significant variation in the genotypes. In CF<sub>Or-HM</sub> genotypes, chromoplasts exhibited a membrane-like structure, but in CF<sub>Or-HT</sub>, they were small granules. The number of chromoplasts was significantly higher in CF<sub>Or-HM</sub> compared to CF<sub>Or-HT</sub>. The CF<sub>WT</sub> had leucoplasts instead of chromoplasts. The CF<sub>Or-HM</sub> (15.1 ± 0.1 µg/g FW) had significantly higher β-carotene content than CF<sub>Or-HT</sub> (5.6 ± 0.3 µg/g FW). Both CF<sub>Or-HM</sub> and CF<sub>Or-HT</sub> had 18 and 7 times higher β-carotene than the white counterpart CF<sub>WT</sub> (0.8 ± 0.1 µg/g FW). The number and size of chromoplasts exhibit a strong correlation with the concentration of total carotenoids and β-carotene in the curd portion. This is the first systematic report on changes in chromoplast features associated with Or-gene zygosity in cauliflower.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transmission electron microscopy reveals the impact of Or gene on chromoplast morphology and β-carotene accumulation in Or gene introgressed tropical cauliflower.\",\"authors\":\"Anamika Chandel, Shrawan Singh\",\"doi\":\"10.1007/s00709-025-02106-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cauliflower Or gene governs massive accumulation of β-carotene in the edible 'curd' portion. However, homozygous dominant (OrOr) plants accumulate higher β-carotene than heterozygous (Oror) individuals, yet this phenomenon has not been thoroughly elucidated in relation to chromoplast morphology. A study was performed in a complete randomised block design with three replicates, in which curd samples of homozygous orange (OrOr; CF<sub>Or-HM</sub>)<sub>,</sub> heterozygous orange (Oror; CF<sub>Or-HT</sub>) and white (oror; CF<sub>WT</sub>) genotypes were analysed using transmission electron microscopy (TEM). The number of chromoplasts in a cell and their morphology (shape and size) exhibited significant variation in the genotypes. In CF<sub>Or-HM</sub> genotypes, chromoplasts exhibited a membrane-like structure, but in CF<sub>Or-HT</sub>, they were small granules. The number of chromoplasts was significantly higher in CF<sub>Or-HM</sub> compared to CF<sub>Or-HT</sub>. The CF<sub>WT</sub> had leucoplasts instead of chromoplasts. The CF<sub>Or-HM</sub> (15.1 ± 0.1 µg/g FW) had significantly higher β-carotene content than CF<sub>Or-HT</sub> (5.6 ± 0.3 µg/g FW). Both CF<sub>Or-HM</sub> and CF<sub>Or-HT</sub> had 18 and 7 times higher β-carotene than the white counterpart CF<sub>WT</sub> (0.8 ± 0.1 µg/g FW). The number and size of chromoplasts exhibit a strong correlation with the concentration of total carotenoids and β-carotene in the curd portion. This is the first systematic report on changes in chromoplast features associated with Or-gene zygosity in cauliflower.</p>\",\"PeriodicalId\":20731,\"journal\":{\"name\":\"Protoplasma\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-08-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Protoplasma\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00709-025-02106-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Protoplasma","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00709-025-02106-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Transmission electron microscopy reveals the impact of Or gene on chromoplast morphology and β-carotene accumulation in Or gene introgressed tropical cauliflower.
Cauliflower Or gene governs massive accumulation of β-carotene in the edible 'curd' portion. However, homozygous dominant (OrOr) plants accumulate higher β-carotene than heterozygous (Oror) individuals, yet this phenomenon has not been thoroughly elucidated in relation to chromoplast morphology. A study was performed in a complete randomised block design with three replicates, in which curd samples of homozygous orange (OrOr; CFOr-HM), heterozygous orange (Oror; CFOr-HT) and white (oror; CFWT) genotypes were analysed using transmission electron microscopy (TEM). The number of chromoplasts in a cell and their morphology (shape and size) exhibited significant variation in the genotypes. In CFOr-HM genotypes, chromoplasts exhibited a membrane-like structure, but in CFOr-HT, they were small granules. The number of chromoplasts was significantly higher in CFOr-HM compared to CFOr-HT. The CFWT had leucoplasts instead of chromoplasts. The CFOr-HM (15.1 ± 0.1 µg/g FW) had significantly higher β-carotene content than CFOr-HT (5.6 ± 0.3 µg/g FW). Both CFOr-HM and CFOr-HT had 18 and 7 times higher β-carotene than the white counterpart CFWT (0.8 ± 0.1 µg/g FW). The number and size of chromoplasts exhibit a strong correlation with the concentration of total carotenoids and β-carotene in the curd portion. This is the first systematic report on changes in chromoplast features associated with Or-gene zygosity in cauliflower.
期刊介绍：
Protoplasma publishes original papers, short communications and review articles which are of interest to cell biology in all its scientific and applied aspects. We seek contributions dealing with plants and animals but also prokaryotes, protists and fungi, from the following fields:
cell biology of both single and multicellular organisms
molecular cytology
the cell cycle
membrane biology including biogenesis, dynamics, energetics and electrophysiology
inter- and intracellular transport
the cytoskeleton
organelles
experimental and quantitative ultrastructure
cyto- and histochemistry
Further, conceptual contributions such as new models or discoveries at the cutting edge of cell biology research will be published under the headings "New Ideas in Cell Biology".
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