拟南芥DJ-1亲缘关系对热应激和氧化应激适应的调控。

IF 3.8 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Priyanka Kataria, Naga Jyothi Pullagurla, Debabrata Laha, Patrick D'Silva
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引用次数: 0

摘要

植物的生长发育是一个高度调控的过程,主要受各种环境因素的控制,这些环境因素的极端暴露导致慢性应激条件,促进活性氧(ROS)和羰基物种(RCS)的产生。ROS和RCS广泛破坏细胞生物分子和细胞器,影响植物的发育。新出现的报告强调,多重应激反应的DJ-1超家族蛋白在减弱与非生物应激相关的细胞毒性作用方面至关重要。目前的报告在酵母和植物模型中得到验证,表明AtDJ-1C和AtDJ-1E是强大的抗氧化剂,可以清除ROS并提高氧化应激下的存活率。虽然它们缺乏传统的乙二醛酶,也不会减弱蛋白质的糖基化,但AtDJ-1C和AtDJ-1E保存GSH库并调节氧化还原稳态。此外,基因表达谱表明,AtDJ-1C和AtDJ-1E的水平可以迅速建立,以对抗高温和氧化应激条件。值得注意的是,atdj - 1c和AtDJ-1E的下调促进了有害的改变,如叶绿素保留减少,根形态发生受损,以及由于ROS升高而诱导对热应激的敏感性。而在生理条件下,过表达AtDJ-1C和AtDJ-1E可提高植株高度和莲座形成。总之,我们的研究揭示了拟南芥DJ-1C和DJ-1E在高温和氧化胁迫条件下调控植物健康和生存的关键功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Control of heat and oxidative stress adaptation by the DJ-1 paralogs in Arabidopsis thaliana.

Plant growth and development are highly regulated processes and are majorly controlled by various environmental factors, whose extreme exposures lead to chronic stress conditions promoting reactive oxygen species (ROS) and carbonyl species (RCS) production. ROS and RCS extensively damage cellular biomolecules and organelles, affecting a plant's development. Emerging reports highlight that the multi-stress responding DJ-1 superfamily proteins are critical in attenuating cytotoxic effects associated with abiotic stress. The current report, validated in yeast and plant models, shows that AtDJ-1C and AtDJ-1E are robust antioxidants that scavenge ROS and improve survival under oxidative stress. Although they lack conventional glyoxalases and do not attenuate the glycation of proteins, AtDJ-1C and AtDJ-1E preserve the GSH pool and regulate redox homeostasis. Moreover, gene expression profiling indicates that levels of AtDJ-1C and AtDJ-1E are rapidly established to counter heat and oxidative stress conditions. Notably, the knockdown of AtDJ-1 C and AtDJ-1E promotes detrimental alterations such as reduced chlorophyll retention, impaired root morphogenesis, and induced sensitivity to heat stress due to ROS elevation. Contrastingly, overexpression of AtDJ-1C and AtDJ-1E improved plant height and rosette formation under physiological conditions. In conclusion, our study unravels the pivotal functions of Arabidopsis thaliana DJ-1C and DJ-1E in governing plant health and survival under heat and oxidative stress conditions.

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来源期刊
Plant Molecular Biology
Plant Molecular Biology 生物-生化与分子生物学
自引率
2.00%
发文量
95
审稿时长
1.4 months
期刊介绍: Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.
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