{"title":"模拟2型糖尿病的SDT fa/fa大鼠骨质和牙槽骨的组织化学评价。","authors":"Tomoka Hasegawa, Mako Sakakibara, Xuanyu Liu, Mai Haraguchi-Kitakamae, Weisong Li, Haoyu Wang, Yan Shi, Jiaxin Cui, Hiromi Hongo, Tomomaya Yamamoto, Miki Abe-Takagi, Haruhi Maruoka, Hirofumi Miyaji, Minqi Li, Norio Amizuka","doi":"10.1111/odi.70079","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the histopathology of diabetic periodontal tissues, we examined periodontal disease in Torii-Lepr<sup>fa</sup> (SDT fa/fa) rats, mimicking type 2 diabetes.</p><p><strong>Methods: </strong>Forty 30-week-old male SDT fa/fa rats and age-matched Sprague-Dawley (SD) rats were fixed, and the mandibular first molars and their periodontal tissues were histochemically examined.</p><p><strong>Results: </strong>SDT fa/fa rats exhibited epithelial downgrowth in the previous region of interradicular/interalveolar septa and periodontal spaces. Many osteoclasts and osteoblasts were present on the alveolar bone, indicating high bone turnover. Masses of bacteria were predominantly observed in the necrotic dental pulps and the cementum, where most cementocytic lacunae were empty. The cementum of the SDT fa/fa rats showed a distinct immunolocalization of osteocalcin, osteopontin, and dentin matrix protein-1 compared with the SD rats, but did not show advanced glycation end products, which were instead detected in some blood vessels and interstitial fibrous tissues associated with the down-growing epithelium.</p><p><strong>Conclusions: </strong>SDT fa/fa rats exhibited necrosis of dental pulp and severe periodontitis featuring markedly diminished alveolar bone because of stimulated bone resorption and formation. In addition, dead cementum may serve as bacterial nests, leading to changes in the localization of cementum matrix proteins.</p>","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Histochemical Assessment of Cementum and Alveolar Bone in SDT fa/fa Rat Mimicking Type II Diabetes.\",\"authors\":\"Tomoka Hasegawa, Mako Sakakibara, Xuanyu Liu, Mai Haraguchi-Kitakamae, Weisong Li, Haoyu Wang, Yan Shi, Jiaxin Cui, Hiromi Hongo, Tomomaya Yamamoto, Miki Abe-Takagi, Haruhi Maruoka, Hirofumi Miyaji, Minqi Li, Norio Amizuka\",\"doi\":\"10.1111/odi.70079\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the histopathology of diabetic periodontal tissues, we examined periodontal disease in Torii-Lepr<sup>fa</sup> (SDT fa/fa) rats, mimicking type 2 diabetes.</p><p><strong>Methods: </strong>Forty 30-week-old male SDT fa/fa rats and age-matched Sprague-Dawley (SD) rats were fixed, and the mandibular first molars and their periodontal tissues were histochemically examined.</p><p><strong>Results: </strong>SDT fa/fa rats exhibited epithelial downgrowth in the previous region of interradicular/interalveolar septa and periodontal spaces. Many osteoclasts and osteoblasts were present on the alveolar bone, indicating high bone turnover. Masses of bacteria were predominantly observed in the necrotic dental pulps and the cementum, where most cementocytic lacunae were empty. The cementum of the SDT fa/fa rats showed a distinct immunolocalization of osteocalcin, osteopontin, and dentin matrix protein-1 compared with the SD rats, but did not show advanced glycation end products, which were instead detected in some blood vessels and interstitial fibrous tissues associated with the down-growing epithelium.</p><p><strong>Conclusions: </strong>SDT fa/fa rats exhibited necrosis of dental pulp and severe periodontitis featuring markedly diminished alveolar bone because of stimulated bone resorption and formation. In addition, dead cementum may serve as bacterial nests, leading to changes in the localization of cementum matrix proteins.</p>\",\"PeriodicalId\":19615,\"journal\":{\"name\":\"Oral diseases\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-08-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oral diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/odi.70079\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/odi.70079","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Histochemical Assessment of Cementum and Alveolar Bone in SDT fa/fa Rat Mimicking Type II Diabetes.
Objective: To investigate the histopathology of diabetic periodontal tissues, we examined periodontal disease in Torii-Leprfa (SDT fa/fa) rats, mimicking type 2 diabetes.
Methods: Forty 30-week-old male SDT fa/fa rats and age-matched Sprague-Dawley (SD) rats were fixed, and the mandibular first molars and their periodontal tissues were histochemically examined.
Results: SDT fa/fa rats exhibited epithelial downgrowth in the previous region of interradicular/interalveolar septa and periodontal spaces. Many osteoclasts and osteoblasts were present on the alveolar bone, indicating high bone turnover. Masses of bacteria were predominantly observed in the necrotic dental pulps and the cementum, where most cementocytic lacunae were empty. The cementum of the SDT fa/fa rats showed a distinct immunolocalization of osteocalcin, osteopontin, and dentin matrix protein-1 compared with the SD rats, but did not show advanced glycation end products, which were instead detected in some blood vessels and interstitial fibrous tissues associated with the down-growing epithelium.
Conclusions: SDT fa/fa rats exhibited necrosis of dental pulp and severe periodontitis featuring markedly diminished alveolar bone because of stimulated bone resorption and formation. In addition, dead cementum may serve as bacterial nests, leading to changes in the localization of cementum matrix proteins.
期刊介绍：
Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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