产生新的Slc20a2敲除小鼠系作为原发性脑钙化的体内模型。

IF 2.9 3区 医学 Q2 NEUROSCIENCES
Hisaka Kurita, Hiroki Kitaura, Kazuya Nishii, Tomohiko Masaka, Kazuki Ohuchi, Masatoshi Inden, Akiyoshi Kakita, Masatake Osawa, Isao Hozumi
{"title":"产生新的Slc20a2敲除小鼠系作为原发性脑钙化的体内模型。","authors":"Hisaka Kurita, Hiroki Kitaura, Kazuya Nishii, Tomohiko Masaka, Kazuki Ohuchi, Masatoshi Inden, Akiyoshi Kakita, Masatake Osawa, Isao Hozumi","doi":"10.1186/s13041-025-01240-8","DOIUrl":null,"url":null,"abstract":"<p><p>Primary brain calcification (PBC) is a neurodegenerative disease that causes bilateral ectopic calcification in the brain. In this study, using newly generated Slc20a2 knockout (Slc20a2<sup>-/-</sup>) mice, we establish an in vivo model for PBC. In contrast to heterozygous Slc20a2<sup>+/-</sup> mice (9/9 animals) showing no obvious abnormalities, the homozygous Slc20a2<sup>-/-</sup> mice exhibited severe calcification at 11 months of age (5/5 animals). Whilst smaller in size and number, the deposits were also detectable in 5-month-old Slc20a2<sup>-/-</sup> mice (2/2 animals). By contrast, no obvious alterations were detectable in visceral organs, including the lung, kidney, liver, and spleen. Consistently, in PBC patients, despite the systemic mineral metabolic disturbance, calcification occurs only in a brain restricted manner. Hence, these observations suggest that our mouse model is capable of recapitulating certain aspects of human PBC etiology. In summary, our data suggested the utility of an in vivo PBC mouse model in understanding the pathological mechanisms behind brain calcification, which leads in development of novel therapeutics against PBC.</p>","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":"18 1","pages":"70"},"PeriodicalIF":2.9000,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369223/pdf/","citationCount":"0","resultStr":"{\"title\":\"Generation of a new Slc20a2 knockout mouse line as in vivo model for primary brain calcification.\",\"authors\":\"Hisaka Kurita, Hiroki Kitaura, Kazuya Nishii, Tomohiko Masaka, Kazuki Ohuchi, Masatoshi Inden, Akiyoshi Kakita, Masatake Osawa, Isao Hozumi\",\"doi\":\"10.1186/s13041-025-01240-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Primary brain calcification (PBC) is a neurodegenerative disease that causes bilateral ectopic calcification in the brain. In this study, using newly generated Slc20a2 knockout (Slc20a2<sup>-/-</sup>) mice, we establish an in vivo model for PBC. In contrast to heterozygous Slc20a2<sup>+/-</sup> mice (9/9 animals) showing no obvious abnormalities, the homozygous Slc20a2<sup>-/-</sup> mice exhibited severe calcification at 11 months of age (5/5 animals). Whilst smaller in size and number, the deposits were also detectable in 5-month-old Slc20a2<sup>-/-</sup> mice (2/2 animals). By contrast, no obvious alterations were detectable in visceral organs, including the lung, kidney, liver, and spleen. Consistently, in PBC patients, despite the systemic mineral metabolic disturbance, calcification occurs only in a brain restricted manner. Hence, these observations suggest that our mouse model is capable of recapitulating certain aspects of human PBC etiology. In summary, our data suggested the utility of an in vivo PBC mouse model in understanding the pathological mechanisms behind brain calcification, which leads in development of novel therapeutics against PBC.</p>\",\"PeriodicalId\":18851,\"journal\":{\"name\":\"Molecular Brain\",\"volume\":\"18 1\",\"pages\":\"70\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369223/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Brain\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s13041-025-01240-8\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Brain","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13041-025-01240-8","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

原发性脑钙化(PBC)是一种神经退行性疾病,导致双侧脑异位钙化。本研究利用新生成的Slc20a2敲除小鼠(Slc20a2-/-),建立PBC的体内模型。与杂合子Slc20a2+/-小鼠(9/9)无明显异常相比,纯合子Slc20a2-/-小鼠在11月龄时出现严重的钙化(5/5)。虽然体积和数量较小,但在5个月大的Slc20a2-/-小鼠(2/2动物)中也可检测到沉积物。相比之下,肺、肾、肝、脾等内脏器官未见明显改变。在PBC患者中,尽管存在全身性矿物质代谢紊乱,但钙化只在脑受限的情况下发生。因此,这些观察结果表明,我们的小鼠模型能够概括人类PBC病因的某些方面。总之,我们的数据表明,PBC小鼠体内模型有助于理解脑钙化背后的病理机制,从而开发出针对PBC的新疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Generation of a new Slc20a2 knockout mouse line as in vivo model for primary brain calcification.

Primary brain calcification (PBC) is a neurodegenerative disease that causes bilateral ectopic calcification in the brain. In this study, using newly generated Slc20a2 knockout (Slc20a2-/-) mice, we establish an in vivo model for PBC. In contrast to heterozygous Slc20a2+/- mice (9/9 animals) showing no obvious abnormalities, the homozygous Slc20a2-/- mice exhibited severe calcification at 11 months of age (5/5 animals). Whilst smaller in size and number, the deposits were also detectable in 5-month-old Slc20a2-/- mice (2/2 animals). By contrast, no obvious alterations were detectable in visceral organs, including the lung, kidney, liver, and spleen. Consistently, in PBC patients, despite the systemic mineral metabolic disturbance, calcification occurs only in a brain restricted manner. Hence, these observations suggest that our mouse model is capable of recapitulating certain aspects of human PBC etiology. In summary, our data suggested the utility of an in vivo PBC mouse model in understanding the pathological mechanisms behind brain calcification, which leads in development of novel therapeutics against PBC.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular Brain
Molecular Brain NEUROSCIENCES-
CiteScore
7.30
自引率
0.00%
发文量
97
审稿时长
>12 weeks
期刊介绍: Molecular Brain is an open access, peer-reviewed journal that considers manuscripts on all aspects of studies on the nervous system at the molecular, cellular, and systems level providing a forum for scientists to communicate their findings. Molecular brain research is a rapidly expanding research field in which integrative approaches at the genetic, molecular, cellular and synaptic levels yield key information about the physiological and pathological brain. These studies involve the use of a wide range of modern techniques in molecular biology, genomics, proteomics, imaging and electrophysiology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信