壳聚糖-甘油混合纳米纤维在周围神经再生中的应用。

IF 4.6 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
L. Scaccini, A. Sensini, D. Puppi, M. Gagliardi, L. Moroni, M. Cecchini, P. Wieringa and I. Tonazzini
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引用次数: 0

摘要

近十年来,壳聚糖作为一种具有生物相容性、可生物降解性、非免疫原性和广泛应用的天然高分子材料,在再生医学领域受到越来越多的关注。然而,虽然壳聚糖支架(如纳米纤维、薄膜等)已经通过多种方法开发出来,但由于壳聚糖材料的脆性和易碎性,这些支架的应用受到了阻碍。壳聚糖塑化可以作为改善机械性能的一种手段,在固体薄膜中掺入甘油已经证明了其提高壳聚糖机械性能的潜力。在这项工作中,我们报道了首次将增塑化壳聚糖用于纳米纤维的生产。采用静电纺丝法制备了定向甘油增塑壳聚糖纳米纤维。用甘油作为增塑剂调节壳聚糖的力学性能。对制备工艺和甘油浓度进行了优化,并对其表面润湿性、红外光谱和热性能进行了测试。我们获得了连续的、无缺陷的Gly (2%)-Chi纳米纤维,直径为184±6 nm,具有高水平的各向异性。这些Gly-Chi排列的纳米纤维在体外也表现出与原代雪旺细胞的生物相容性,并有效地引导其极化,从而表明它们在周围神经再生方面的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chitosan–glycerol blended nanofibers for peripheral nerve regeneration applications

Chitosan–glycerol blended nanofibers for peripheral nerve regeneration applications

Over the last decade, chitosan has drawn increasing attention in the field of regenerative medicine as a natural polymer that is biocompatible, biodegradable, non-immunogenic and widely available. However, while chitosan scaffolds (e.g. nanofibers, films, etc.) have been developed by multiple methods, the application of these scaffolds has been hampered by the brittle and fragile nature of this material. Chitosan plasticization is possible as a means of improving mechanical properties, with the incorporation of glycerol within solid films having demonstrated its potential to enhance the mechanical properties of chitosan. In this work, we report the first use of plasticized chitosan for the production of nanofibers. We produced aligned glycerol plasticized chitosan nanofibers (Gly–Chi NFs) using electrospinning. Glycerol was used as a plasticizer in order to modulate the mechanical properties of chitosan. We optimized the production process and the glycerol concentration, and measured the surface wettability, FT-IR spectra and thermal properties of the NFs. We obtained continuous, defect-free Gly (2%)–Chi nanofibers with a diameter of 184 ± 6 nm and a high level of anisotropy. These Gly–Chi aligned nanofibers also exhibited biocompatibility with primary Schwann cells in vitro, and were effective in guiding their bipolarization, thus suggesting their potential for peripheral nerve regeneration applications.

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来源期刊
Nanoscale Advances
Nanoscale Advances Multiple-
CiteScore
8.00
自引率
2.10%
发文量
461
审稿时长
9 weeks
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