CRISPR-Cas基因编辑系统在根除人类微生物病原体方面的进展

IF 2.5 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-08-20 DOI:10.1007/s12033-025-01482-w

Gargi Bhattacharjee, Nisarg Gohil, Khushal Khambhati, Karan Murjani, Dinh Toi Chu, Nhat Le Bui, Hue Vu Thi, Indra Mani, Abhisheka Bansal, Sasanala Shamili, Lakkakula Satish, Suresh Ramakrishna, Khalid J Alzahrani, Vijai Singh

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引用次数: 0

摘要

CRISPR-Cas系统已被探索用于几种生物的靶向基因组编辑。它是一种快速、经济、专门和通用的技术。它需要表达多结构域单Cas9蛋白和单向导RNA (sgRNA)，在原间隔器邻近基序（PAM）存在的情况下靶向所需的核酸。这会产生双链断裂，通过非同源末端连接或同源定向修复途径进行修复。目前，已经发现了几种Cas蛋白变体，并被用于几种生物技术应用。本文综述了CRISPR-Cas系统在主要用于控制感染的人类致病微生物基因组编辑方面的最新进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Advancements in CRISPR-Cas Systems for Genome Editing towards Eradication of Human Microbial Pathogens.

CRISPR-Cas systems have been explored for targeted genome editing of several organisms. It is rapid, cost-effective, specific, and versatile technology. It requires expression of multidomain single Cas9 protein and single guide RNA (sgRNA) that targets desired nucleic acids in the presence of a protospacer adjacent motif (PAM). This generates a double-stranded break that is repaired by either non-homologous end joining or a homology-directed repair pathway. Currently, several Cas protein variants have been discovered and being used for several biotechnological applications. This review highlights the recent progress of CRISPR-Cas systems for genome editing of mainly human pathogenic microorganisms for their controlling infections.

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.