非肌球蛋白IIA (NMIIA)调节各向异性细胞张力,维持小鼠晶状体子午线细胞的六边形排列。

IF 2.7 3区 生物学 Q3 CELL BIOLOGY
Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-20 DOI:10.1091/mbc.E25-04-0154
Sadia T Islam, Yiwen Tang, Heather Boliver, Dapeng Bi, Velia M Fowler
{"title":"非肌球蛋白IIA (NMIIA)调节各向异性细胞张力,维持小鼠晶状体子午线细胞的六边形排列。","authors":"Sadia T Islam, Yiwen Tang, Heather Boliver, Dapeng Bi, Velia M Fowler","doi":"10.1091/mbc.E25-04-0154","DOIUrl":null,"url":null,"abstract":"<p><p>The mouse ocular lens is an excellent vertebrate model for epithelial cell hexagonal packing during tissue morphogenesis. As lens epithelial cells differentiate into fiber cells, the epithelial cells rearrange into hexagonally packed meridional row (MR) cells that further differentiate to form fiber cells. We previously reported that the nonmuscle myosin IIA (NMIIA)-E1841K mutation, which alters NMIIA bipolar filament assembly, significantly disrupts MR cell hexagonal packing. Immunofluorescence microscopy of MR cells demonstrates increased enrichment of NMIIA, N-cadherin, and vinculin at anterior-posterior (AP)-oriented sides of control MR cells, but equal distributions on all sides of mutant MR cells. Furthermore, F-actin is uniformly distributed around all edges of control MR cells but reduced at the AP-oriented edges of mutant MR cells. Using Bayesian Mechanical Inference, we discovered that MR cells in control lenses exhibit anisotropic junctional tension, in which relative tension is more concentrated at the AP-oriented edges. In contrast, MR cells in mutant lenses show isotropic junctional tension on all sides. We conclude that the NMIIA-E1841K mutation results in altered F-actin, NMIIA, N-cadherin, and vinculin distributions, disrupting the anisotropic orientational pattern of mechanical forces within the tissue, leading to disordered cell packing during mouse lens epithelial cell differentiation.</p>","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"ar124"},"PeriodicalIF":2.7000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483326/pdf/","citationCount":"0","resultStr":"{\"title\":\"Nonmuscle myosin IIA (NMIIA) regulates anisotropic cell tension to maintain the hexagonal packing of mouse lens meridional row cells.\",\"authors\":\"Sadia T Islam, Yiwen Tang, Heather Boliver, Dapeng Bi, Velia M Fowler\",\"doi\":\"10.1091/mbc.E25-04-0154\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The mouse ocular lens is an excellent vertebrate model for epithelial cell hexagonal packing during tissue morphogenesis. As lens epithelial cells differentiate into fiber cells, the epithelial cells rearrange into hexagonally packed meridional row (MR) cells that further differentiate to form fiber cells. We previously reported that the nonmuscle myosin IIA (NMIIA)-E1841K mutation, which alters NMIIA bipolar filament assembly, significantly disrupts MR cell hexagonal packing. Immunofluorescence microscopy of MR cells demonstrates increased enrichment of NMIIA, N-cadherin, and vinculin at anterior-posterior (AP)-oriented sides of control MR cells, but equal distributions on all sides of mutant MR cells. Furthermore, F-actin is uniformly distributed around all edges of control MR cells but reduced at the AP-oriented edges of mutant MR cells. Using Bayesian Mechanical Inference, we discovered that MR cells in control lenses exhibit anisotropic junctional tension, in which relative tension is more concentrated at the AP-oriented edges. In contrast, MR cells in mutant lenses show isotropic junctional tension on all sides. We conclude that the NMIIA-E1841K mutation results in altered F-actin, NMIIA, N-cadherin, and vinculin distributions, disrupting the anisotropic orientational pattern of mechanical forces within the tissue, leading to disordered cell packing during mouse lens epithelial cell differentiation.</p>\",\"PeriodicalId\":18735,\"journal\":{\"name\":\"Molecular Biology of the Cell\",\"volume\":\" \",\"pages\":\"ar124\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483326/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Biology of the Cell\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1091/mbc.E25-04-0154\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Biology of the Cell","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1091/mbc.E25-04-0154","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/20 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

小鼠晶状体是研究组织形态发生过程中上皮细胞六角形堆积的良好脊椎动物模型。晶状体上皮细胞分化为纤维细胞时,上皮细胞重新排列成六边形排列的子午行细胞,进一步分化为纤维细胞。我们之前报道了非肌肉肌球蛋白IIA (NMIIA)-E1841K突变,它改变了NMIIA双极丝的组装,显著破坏了MR细胞的六边形堆积。MR细胞的免疫荧光显微镜显示,在对照MR细胞的前后(AP)定向侧,NMIIA、N-cadherin和vinculin的富集增加,但在突变MR细胞的所有侧分布相同。此外,f -肌动蛋白均匀分布在对照MR细胞的所有边缘,但在突变MR细胞的ap方向边缘减少。利用贝叶斯力学推理,我们发现对照透镜中的MR细胞表现出各向异性的连接张力,其中相对张力更集中在ap取向边缘。相反,突变体中的MR细胞在所有方面都表现出各向同性的连接张力。我们得出结论,NMIIA- e1841k突变导致f -肌动蛋白、NMIIA、n -钙粘蛋白和血管蛋白分布改变,破坏组织内机械力的各向异性取向模式,导致小鼠晶状体上皮细胞分化过程中细胞堆积紊乱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nonmuscle myosin IIA (NMIIA) regulates anisotropic cell tension to maintain the hexagonal packing of mouse lens meridional row cells.

The mouse ocular lens is an excellent vertebrate model for epithelial cell hexagonal packing during tissue morphogenesis. As lens epithelial cells differentiate into fiber cells, the epithelial cells rearrange into hexagonally packed meridional row (MR) cells that further differentiate to form fiber cells. We previously reported that the nonmuscle myosin IIA (NMIIA)-E1841K mutation, which alters NMIIA bipolar filament assembly, significantly disrupts MR cell hexagonal packing. Immunofluorescence microscopy of MR cells demonstrates increased enrichment of NMIIA, N-cadherin, and vinculin at anterior-posterior (AP)-oriented sides of control MR cells, but equal distributions on all sides of mutant MR cells. Furthermore, F-actin is uniformly distributed around all edges of control MR cells but reduced at the AP-oriented edges of mutant MR cells. Using Bayesian Mechanical Inference, we discovered that MR cells in control lenses exhibit anisotropic junctional tension, in which relative tension is more concentrated at the AP-oriented edges. In contrast, MR cells in mutant lenses show isotropic junctional tension on all sides. We conclude that the NMIIA-E1841K mutation results in altered F-actin, NMIIA, N-cadherin, and vinculin distributions, disrupting the anisotropic orientational pattern of mechanical forces within the tissue, leading to disordered cell packing during mouse lens epithelial cell differentiation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular Biology of the Cell
Molecular Biology of the Cell 生物-细胞生物学
CiteScore
6.00
自引率
6.10%
发文量
402
审稿时长
2 months
期刊介绍: MBoC publishes research articles that present conceptual advances of broad interest and significance within all areas of cell, molecular, and developmental biology. We welcome manuscripts that describe advances with applications across topics including but not limited to: cell growth and division; nuclear and cytoskeletal processes; membrane trafficking and autophagy; organelle biology; quantitative cell biology; physical cell biology and mechanobiology; cell signaling; stem cell biology and development; cancer biology; cellular immunology and microbial pathogenesis; cellular neurobiology; prokaryotic cell biology; and cell biology of disease.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信