Circ_0000847通过与IGF2BP2结合,增强IGF2 mRNA稳定性,促进结直肠癌的迁移、侵袭和EMT过程。

IF 3.5 4区 医学 Q2 ONCOLOGY
Aikang Zhang, Yongbin Zheng
{"title":"Circ_0000847通过与IGF2BP2结合,增强IGF2 mRNA稳定性,促进结直肠癌的迁移、侵袭和EMT过程。","authors":"Aikang Zhang, Yongbin Zheng","doi":"10.1007/s12032-025-02877-0","DOIUrl":null,"url":null,"abstract":"<p><p>Colorectal cancer (CRC) stands as one of the most prevalent forms of malignant gastrointestinal tumors. Circular RNAs (circRNAs) could serve as promising targets for therapeutic intervention in CRC. This study aims to uncover the significance and influence of circRNAs within the landscape of CRC. CircRNA microarray, RNase R assay, and fluorescence in situ hybridization were conducted to verify the expression and characteristics of circRNA. The biological behaviors of CRC cells were evaluated by cell counting kit-8, transwell assay, and wound-healing assay. The underlying mechanism was revealed using quantitative real-time PCR, Western bot, silver staining assay, biotin-labeled RNA pulldown, and RNA immunoprecipitation. The results indicated that circ_0000847, a CRC-associated circRNA, was over-expressed in CRC. Inhibition of circ_0000847 effectively restrained the migratory and invasive behaviors of CRC cells, alongside suppressing their epithelial-mesenchymal transition (EMT). Circ_0000847 bound to IGF2BP2, thereby enhancing the IGF2 mRNA stability. Importantly, overexpression of IGF2 abrogated the inhibitory effect of circ_0000847 depletion on CRC cellular processes. In addition, circ_0000847 promoted tumor growth in vivo. In summary, by engaging with IGF2BP2, circ_0000847 plays a role in stabilizing IGF2 mRNA, which in turn curtails the advancement of CRC. Hence, circ_0000847 could serve as an innovative and potentially pivotal target for the development of CRC therapies.</p>","PeriodicalId":18433,"journal":{"name":"Medical Oncology","volume":"42 10","pages":"436"},"PeriodicalIF":3.5000,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Circ_0000847 promotes the migration, invasion, and EMT process in colorectal cancer through binding to IGF2BP2 to enhance IGF2 mRNA stability.\",\"authors\":\"Aikang Zhang, Yongbin Zheng\",\"doi\":\"10.1007/s12032-025-02877-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Colorectal cancer (CRC) stands as one of the most prevalent forms of malignant gastrointestinal tumors. Circular RNAs (circRNAs) could serve as promising targets for therapeutic intervention in CRC. This study aims to uncover the significance and influence of circRNAs within the landscape of CRC. CircRNA microarray, RNase R assay, and fluorescence in situ hybridization were conducted to verify the expression and characteristics of circRNA. The biological behaviors of CRC cells were evaluated by cell counting kit-8, transwell assay, and wound-healing assay. The underlying mechanism was revealed using quantitative real-time PCR, Western bot, silver staining assay, biotin-labeled RNA pulldown, and RNA immunoprecipitation. The results indicated that circ_0000847, a CRC-associated circRNA, was over-expressed in CRC. Inhibition of circ_0000847 effectively restrained the migratory and invasive behaviors of CRC cells, alongside suppressing their epithelial-mesenchymal transition (EMT). Circ_0000847 bound to IGF2BP2, thereby enhancing the IGF2 mRNA stability. Importantly, overexpression of IGF2 abrogated the inhibitory effect of circ_0000847 depletion on CRC cellular processes. In addition, circ_0000847 promoted tumor growth in vivo. In summary, by engaging with IGF2BP2, circ_0000847 plays a role in stabilizing IGF2 mRNA, which in turn curtails the advancement of CRC. Hence, circ_0000847 could serve as an innovative and potentially pivotal target for the development of CRC therapies.</p>\",\"PeriodicalId\":18433,\"journal\":{\"name\":\"Medical Oncology\",\"volume\":\"42 10\",\"pages\":\"436\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medical Oncology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s12032-025-02877-0\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical Oncology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12032-025-02877-0","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

结直肠癌(CRC)是最常见的恶性胃肠道肿瘤之一。环状rna (circRNAs)可以作为CRC治疗干预的有希望的靶点。本研究旨在揭示环状rna在结直肠癌中的意义和影响。通过CircRNA芯片、RNase R检测和荧光原位杂交验证CircRNA的表达和特性。采用细胞计数试剂盒-8、transwell实验和创面愈合实验评价结直肠癌细胞的生物学行为。通过实时荧光定量PCR、Western bot、银染色、生物素标记RNA下拉和RNA免疫沉淀等方法揭示其潜在机制。结果表明,CRC相关circRNA circ_0000847在CRC中过表达。抑制circ_0000847可有效抑制结直肠癌细胞的迁移和侵袭行为,同时抑制其上皮-间质转化(EMT)。Circ_0000847与IGF2BP2结合,从而增强了IGF2 mRNA的稳定性。重要的是,IGF2的过表达消除了circ_0000847缺失对CRC细胞过程的抑制作用。此外,circ_0000847在体内促进肿瘤生长。综上所述,circ_0000847通过与IGF2BP2结合,在稳定IGF2 mRNA中发挥作用,从而抑制CRC的进展。因此,circ_0000847可以作为CRC治疗开发的创新和潜在关键靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Circ_0000847 promotes the migration, invasion, and EMT process in colorectal cancer through binding to IGF2BP2 to enhance IGF2 mRNA stability.

Colorectal cancer (CRC) stands as one of the most prevalent forms of malignant gastrointestinal tumors. Circular RNAs (circRNAs) could serve as promising targets for therapeutic intervention in CRC. This study aims to uncover the significance and influence of circRNAs within the landscape of CRC. CircRNA microarray, RNase R assay, and fluorescence in situ hybridization were conducted to verify the expression and characteristics of circRNA. The biological behaviors of CRC cells were evaluated by cell counting kit-8, transwell assay, and wound-healing assay. The underlying mechanism was revealed using quantitative real-time PCR, Western bot, silver staining assay, biotin-labeled RNA pulldown, and RNA immunoprecipitation. The results indicated that circ_0000847, a CRC-associated circRNA, was over-expressed in CRC. Inhibition of circ_0000847 effectively restrained the migratory and invasive behaviors of CRC cells, alongside suppressing their epithelial-mesenchymal transition (EMT). Circ_0000847 bound to IGF2BP2, thereby enhancing the IGF2 mRNA stability. Importantly, overexpression of IGF2 abrogated the inhibitory effect of circ_0000847 depletion on CRC cellular processes. In addition, circ_0000847 promoted tumor growth in vivo. In summary, by engaging with IGF2BP2, circ_0000847 plays a role in stabilizing IGF2 mRNA, which in turn curtails the advancement of CRC. Hence, circ_0000847 could serve as an innovative and potentially pivotal target for the development of CRC therapies.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Medical Oncology
Medical Oncology 医学-肿瘤学
CiteScore
4.20
自引率
2.90%
发文量
259
审稿时长
1.4 months
期刊介绍: Medical Oncology (MO) communicates the results of clinical and experimental research in oncology and hematology, particularly experimental therapeutics within the fields of immunotherapy and chemotherapy. It also provides state-of-the-art reviews on clinical and experimental therapies. Topics covered include immunobiology, pathogenesis, and treatment of malignant tumors.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信