微滤膜稳定高效分离稀有细菌的浮萍-微生物共培养方法的改进。

IF 2 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2025-01-01 DOI:10.1264/jsme2.ME24075

Yosuke Morishita, Tomoki Iwashita, Manabu Kanno, Hideyuki Tamaki, Yoichi Kamagata, Tadashi Toyama, Kazuhiro Mori, Masaaki Morikawa, Yasuhiro Tanaka

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引用次数: 0

摘要

我们最近提出了一种新的微生物分离技术，“浮萍-微生物共培养法”，用于分离各种各样的微生物，包括很少被培养的微生物。这种方法包括无菌浮萍与环境微生物接种，然后共同培养一段时间。然后用传统的琼脂平板法将植物及其周围的培养基作为微生物源进行分离。本研究改进了微滤膜（孔径为0.8 ~ 1.2‍‍μm）对微生物接种物进行预处理的方法，提高了Verrucomicrobiota门微生物的分离效率。

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Improvements in the Duckweed-Microbe Co-cultivation Method for the Stable and Efficient Isolation of Rarely Cultivated Bacteria Using Microfilter Membranes.

We recently proposed a novel microbial isolation technique, the "duckweed-microbe co-cultivation method", for isolating a wide variety of microbes, including rarely cultivated microbes. This method involves the inoculation of aseptic duckweed with environmental microbes followed by co-cultivation for a set period. Plants and their surrounding medium are then used as microbial sources for isolation in the conventional agar plate method. In the present study, we improved the method by using microfilter membranes (pore sizes of 0.8-1.2‍ ‍μm) to pretreat microbial inocula, which increased the isolation efficiency of rarely cultivated microbes representing the phylum Verrucomicrobiota.

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.