新诊断系统性红斑狼疮伴肾脏受累患者单核细胞中TWEAK和CD163的表达及意义

IF 1.9 4区 医学 Q3 RHEUMATOLOGY
Lupus Pub Date : 2025-10-01 Epub Date: 2025-08-25 DOI:10.1177/09612033251371335
Lu Zhang, Lulu Zeng, Tingting Zeng, Qianbin Dai, Yao Zhou, Qing Luo, Junming Li
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The sensitivity and specificity of TWEAK and CD163 for the diagnosis and differential diagnosis of SLE+RI were analysed by receiver operating characteristic (ROC) curves. Western blot experiments were used to evaluate the protein expression of TWEAK and CD163 in monocytes.ResultsThe expression levels of TWEAK and CD163 in monocytes were significantly greater in the SLE group than in the healthy control (HC) and rheumatoid arthritis (RA) groups. The expression levels of TWEAK and CD163 in monocytes from anti-double-stranded DNA antibody (anti-dsDNA)-positive patients and patients with proteinuria were respectively greater than those from anti-dsDNA-negative patients and patients without proteinuria. The expression levels of both genes were significantly lower after treatment than before treatment in the SLE+RI group (<i>p</i> < 0.05). The expression levels of TWEAK and CD163 in monocytes were positively correlated with the SLE activity score (SLEDAI) in the SLE+RI group. 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引用次数: 0

摘要

目的检测系统性红斑狼疮(SLE)合并肾累及(SLE+RI)患者外周血单核细胞中TWEAK和CD163的表达水平,探讨TWEAK和CD163在SLE及SLE+RI诊断中的应用价值。方法采用实时荧光定量聚合酶链反应(RT-qPCR)检测70例SLE患者和对照组单核细胞中TWEAK和CD163的表达水平。分析SLE+RI患者单核细胞中TWEAK/CD163表达水平与实验室检查及临床表现的关系。采用受试者工作特征(ROC)曲线分析TWEAK和CD163对SLE+RI诊断和鉴别诊断的敏感性和特异性。Western blot检测单核细胞中TWEAK和CD163蛋白的表达。结果SLE组单核细胞中TWEAK和CD163的表达水平明显高于健康对照组(HC)和类风湿关节炎(RA)组。抗双链DNA抗体(anti-dsDNA)阳性和蛋白尿患者单核细胞中TWEAK和CD163的表达水平分别高于抗双链DNA抗体阴性和无蛋白尿患者。SLE+RI组治疗后两种基因表达水平均显著低于治疗前(p < 0.05)。在SLE+RI组,单核细胞中TWEAK和CD163的表达水平与SLE活动度评分(SLEDAI)呈正相关。ROC曲线分析显示,SLE+RI组TWEAK表达曲线下面积(AUC)为0.869。SLE+RI组CD163的AUC为0.792,SLE+RI组TWEAK与CD163的联合表达为0.842。与对照组相比,SLE+RI患者单核细胞中TWEAK和CD163蛋白表达显著升高。结论SLE患者中TWEAK和CD163表达水平升高,且SLE+RI患者表达水平高于SLE-RI患者。这些发现与疾病活动性、自身抗体产生和临床症状密切相关,可作为SLE+RI诊断和活动性的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression and significance of TWEAK and CD163 in monocytes of patients with newly diagnosed systemic lupus erythematosus with renal involvement.

ObjectiveTo detect the expression levels of TWEAK and CD163 in monocytes from the peripheral blood of patients with systemic lupus erythematosus (SLE) complicated with renal involvement (SLE+RI) and to explore the application value of TWEAK and CD163 in the diagnosis of SLE and SLE+RI.MethodsThe expression levels of TWEAK and CD163 in the monocytes of 70 SLE patients and the control group were determined by real-time fluorescence quantitative polymerase chain reaction (RT‒qPCR). To analyse the relationship between TWEAK/CD163 expression levels and laboratory examination and clinical manifestations in monocytes of SLE+RI patients. The sensitivity and specificity of TWEAK and CD163 for the diagnosis and differential diagnosis of SLE+RI were analysed by receiver operating characteristic (ROC) curves. Western blot experiments were used to evaluate the protein expression of TWEAK and CD163 in monocytes.ResultsThe expression levels of TWEAK and CD163 in monocytes were significantly greater in the SLE group than in the healthy control (HC) and rheumatoid arthritis (RA) groups. The expression levels of TWEAK and CD163 in monocytes from anti-double-stranded DNA antibody (anti-dsDNA)-positive patients and patients with proteinuria were respectively greater than those from anti-dsDNA-negative patients and patients without proteinuria. The expression levels of both genes were significantly lower after treatment than before treatment in the SLE+RI group (p < 0.05). The expression levels of TWEAK and CD163 in monocytes were positively correlated with the SLE activity score (SLEDAI) in the SLE+RI group. ROC curve analysis revealed that the area under the curve (AUC) of TWEAK expression was 0.869 in the SLE+RI group. The AUC of CD163 in the SLE+RI group was 0.792, the combined expression of TWEAK and CD163 was 0.842 in the SLE+RI group. TWEAK and CD163 protein expression in monocytes from patients with SLE+RI was significantly increased compared with that in controls.ConclusionThe expression levels of TWEAK and CD163 are increased in SLE patients, and the expression levels in SLE+RI patients are greater than those in SLE-RI patients. These findings are closely related to disease activity, autoantibody production and clinical symptoms and can be used as biomarkers for the diagnosis and activity of SLE+RI.

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来源期刊
Lupus
Lupus 医学-风湿病学
CiteScore
4.20
自引率
11.50%
发文量
225
审稿时长
1 months
期刊介绍: The only fully peer reviewed international journal devoted exclusively to lupus (and related disease) research. Lupus includes the most promising new clinical and laboratory-based studies from leading specialists in all lupus-related disciplines. Invaluable reading, with extended coverage, lupus-related disciplines include: Rheumatology, Dermatology, Immunology, Obstetrics, Psychiatry and Cardiovascular Research…
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