Maria A Paularie, Emerson A Fonseca, Vitor Monken, André G Pereira, Rafael P Vieira, Ado Jorio
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Exploring collagen fibrillogenesis at the nanoscale: Tip-enhanced Raman imaging of protofibrils.
Collagen, a key structural component of the extracellular matrix, assembles through a hierarchical process of fibrillogenesis. Despite extensive studies on mature collagen fibrils, intermediates such as protofibrils remain underexplored, particularly at the nanoscale. This study presents hyperspectral tip-enhanced Raman spectroscopy (TERS) imaging of collagen protofibrils, offering chemical and structural insights into early fibrillogenesis by acquiring nanoscale molecular profiles of collagen intermediates. TERS spectra, complemented by atomic force microscopy (AFM) images, reveal characteristic molecular vibrational modes, including the phenylalanine ring breathing mode, amide II and / stretching vibrations, with distinct spectral signatures compared to mature fibrils.
期刊介绍：
The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit.
The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens.
Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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