不同“煮沸”方法提取细菌DNA的效果。

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-08-30 DOI:10.1016/j.mimet.2025.107245

Isabella R. Loberiza , Fletcher E. Falk , Timothy J. Green , Andrew H. Loudon

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引用次数: 0

摘要

16S rRNA基因的Sanger测序仍然是鉴定细菌分离物的有效方法，但首先需要一种有效且廉价的DNA提取方法。我们测试了5种不同的低成本DNA提取方法对9株细菌分离物的提取效果，以确定一种标准方法。

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Efficacy of different “boiling” methods for bacterial DNA extraction

Sanger sequencing of the 16S rRNA gene remains an effective method to identify bacterial isolates but first requires an effective and inexpensive DNA extraction method. We tested the efficacy of five different low-cost DNA extraction methods on nine bacterial isolates to identify a standard method.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.