新型合成肽抗体识别神经内分泌蛋白7B2在内分泌细胞和物种间的表达多样性

IF 1.5 4区 生物学 Q4 CELL BIOLOGY
Journal of Histochemistry & Cytochemistry Pub Date : 2025-07-01 Epub Date: 2025-08-25 DOI:10.1369/00221554251365996
Shota Kikuchi, Seiji Torii, Masahiro Hosaka, Tadashi Yasui, Hiroshi Gomi
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引用次数: 0

摘要

神经内分泌蛋白7B2在激素原转化酶2 (PC2)的成熟和活性调控中起着至关重要的作用。为了阐明7B2与PC2在内分泌组织中的表达关系,我们在豚鼠体内合成了多肽抗体。选择的抗原肽序列对应于大鼠氨基酸(aa)序列的三个不同位置:n端aa 1-14位于信号序列之后,中间aa 77-90包含部分proPC2激活域,c端aa 156-168具有抑制PC2活性的功能。这些抗体在多种动物物种中表现出特异性反应性。这些抗体的反应性不同,表明7B2的分子形式因内分泌细胞而异,在大鼠和狗垂体中间细胞中表现出不同的表达模式。7B2和PC2在大鼠垂体乳腺肥大细胞泌乳素(PRL)颗粒中的共定位支持了这些蛋白之间的相互作用。然而,这些蛋白的表达强度并不对应,并且检测到与表位相关的差异。这些结果可能表明与7B2和PC2相互作用动力学相关的分子状态的改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression Diversity in Endocrine Cells and Between Species Revealed by Novel Synthetic Peptide Antibodies Recognizing the Neuroendocrine Protein 7B2.

The neuroendocrine protein 7B2 plays a crucial role in the maturation and activity regulation of prohormone convertase 2 (PC2). To elucidate the relationship between 7B2 and PC2 expression in endocrine tissues, we generated synthetic peptide antibodies in guinea pigs. The antigenic peptide sequences were selected to correspond to three different positions in the rat amino acid (aa) sequence: The N-terminal aa 1-14 is situated immediately following the signal sequence, the middle aa 77-90 contains a part of the proPC2 activation domain, and the C-terminal aa 156-168 functions to suppress PC2 activity. These antibodies demonstrated specific reactivity across a diverse array of animal species. The reactivity of these antibodies differed, suggesting that the molecular form of 7B2 differs depending on the endocrine cell, and a different expression pattern was demonstrated in rat and dog pituitary intermediate cells. The colocalization of 7B2 and PC2 in prolactin (PRL) granules in rat pituitary mammotrophs supports the interaction between these proteins. However, the expression intensities of these proteins did not correspond, and epitope-related disparities were detected. These results may be indicative of alterations in the molecular state associated with the dynamics of the interaction between 7B2 and PC2.

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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
32
审稿时长
1 months
期刊介绍: Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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