纳米姜黄素介导的光动力疗法作为根管消毒的新方法：离体研究。

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-08-30 DOI:10.1016/j.jdent.2025.106075

Nayrouz B. Soliman , Sally A. Sabra , Shahinda Rezk , Nayera A. Mokhless , Rania M. ElBackly

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引用次数: 0

摘要

背景：抗菌光动力疗法（aPDT）已被推荐用于加强根管消毒。天然衍生的光敏剂被认为可以提高aPDT的有效性。目的：评价纳米姜黄素作为牙髓消毒光敏剂的体外特性、抗菌活性和管状渗透深度。材料与方法：采用溶胶-油法制备纳米姜黄素，并利用紫外可见分光光度法、zeta电位测量、傅里叶变换红外光谱、x射线衍射分析和透射电镜对其进行了表征。采用孔扩散法测定其抑菌活性，确定最小抑菌浓度。单根牙分为3组（n=6）；I组（纳米姜黄素修饰aPDT）、II组（姜黄素修饰aPDT）和III组（亚甲基蓝常规aPDT）。在离根尖4、8、12 mm处制备牙体，在激光共聚焦显微镜下观察管状牙体穿透率。数据收集，制表和统计分析使用合适的测试。结果：制备了一种纯形式的纳米姜黄素并对其进行了表征。纳米姜黄素和姜黄素对粪肠球菌的抑菌作用（p=0.298）和对管状渗透的作用（p=0.662）均无显著差异。与亚甲基蓝相比，纳米姜黄素和姜黄素的穿透性均显著增强（p≤0.001）。结论：与亚甲基蓝相比，纳米姜黄素和姜黄素对微管的穿透性显著提高，但其抑菌活性和穿透深度无显著差异。因此，纳米姜黄素可以取代姜黄素作为光敏剂，因为它具有提高溶解度和消除二甲亚砜作为溶剂的作用。临床意义：纳米姜黄素可作为一种有效的环境友好型光敏剂，加强牙髓感染的抗菌光动力治疗。

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Nanocurcumin mediated photodynamic therapy as a novel approach for root canal disinfection: ex-vivo study

Background

Antibacterial photodynamic therapy (aPDT) has been recommended to enhance root canal disinfection. Naturally derived photosensitizers have been suggested to enhance the effectiveness of aPDT.

Aim

To evaluate the in vitro characteristics, antibacterial activity and tubular penetration depth of nanocurcumin used as a photosensitizer for endodontic disinfection.

Materials and Methods

Nanocurcumin was prepared using sol-oil technique and characterized using ultraviolet visible spectrophotometry, zeta potential measurements, Fourier transform infrared spectroscopy, X-ray diffraction analysis and transmission electron microscopy. The antibacterial activity was assessed using well diffusion test and the minimum inhibitory concentration was determined. Single-rooted teeth were divided into 3 equal groups (n=6); group I (nanocurcumin modified aPDT), group II (curcumin modified aPDT) and group III (methylene blue conventional aPDT). Teeth were prepared and sectioned at 4, 8 and 12 mm from the root apices and examined under confocal laser scanning microscope to evaluate the tubular penetration percentage. Data were collected, tabulated and statistically analyzed using suitable tests.

Results

A pure form of nanocurcumin was prepared and characterized. There was no significant difference between nanocurcumin and curcumin neither in their antibacterial effect on Enterococcus faecalis (p=0.298) nor in their tubular penetration (p=0.662). Both nanocurcumin and curcumin showed significantly enhanced penetration when compared to methylene blue (p≤0.001).

Conclusion

Both nanocurcumin and curcumin, caused significantly higher tubular penetration compared with methylene blue, with no significant difference between them regarding antibacterial activity and penetration depth. Thus nanocurcumin could replace curcumin as a photosensitizer for its enhanced solubility and elimination of dimethyl sulfoxide as a solvent.

Clinical significance

Nanocurcumin can offer an effective environment-friendly photosensitizer for enhancing antibacterial photodynamic therapy for management of endodontic infections.

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.