使用滑膜液和关节组织标本的BioFire关节感染面板的性能和潜在效用。

IF 5.4 2区 医学 Q1 MICROBIOLOGY
Shivani Fox-Lewis, Marc Douglass, Sally Roberts
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引用次数: 0

摘要

BioFire联合感染面板(BJIP) (biomrieux)是一种用于关节感染诊断的快速样本到答案多重聚合酶链反应(PCR)平台。本回顾性研究评估了其在天然关节和假关节的滑液和关节组织标本中的表现。包括2023年11月至2024年9月接收的关节液和组织标本。一些标本在BJIP检测之前被合并。将BJIP结果与复合标准实验室检测(培养、16S rRNA基因PCR和测序,以及该感染事件的并发微生物学结果)进行比较。收集临床资料(诊断、手术史、抗生素治疗)。对天然和人工关节标本进行亚群分析。134例患者224份标本,其中滑液107份,关节组织117份。BJIP检测到的最常见的细菌是金黄色葡萄球菌和链球菌,滑液和关节组织的PPA阳性率分别为90.5%和86.8%。滑膜液与关节组织的PPA无显著差异,天然关节与假体关节的PPA也无显著差异。额外诊断结果为1例。假体关节感染中缺少重要病原体,如痤疮角质杆菌,降低了总体PPA(74.5%的滑液,77.6%的关节组织)。BJIP在滑液和关节组织标本中表现良好,来自天然关节和假关节的面板上生物。很少有额外的诊断结果。我们建议使用BJIP预先提供快速结果以优化患者护理的实施途径。本研究比较了BioFire关节感染聚合酶链反应面板与标准的基于培养的方法来检测引起关节感染的生物体。这是一项相对较新的测试,迄今公布的数据表明,它在滑液中表现良好。有有限的已发表的数据使用它的关节组织标本。我们发现BioFire关节感染面板可以可靠地检测滑液和关节组织标本中的病原体,尽管其性能受到与假体关节感染相关的重要病原体(如痤疮角膜炎)缺失的限制。我们建议使用小组提供快速诊断,以优化患者护理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Performance and potential utility of the BioFire Joint Infection Panel with synovial fluid and joint tissue specimens.

The BioFire Joint Infection Panel (BJIP) (bioMérieux) is a rapid sample-to-answer multiplex polymerase chain reaction (PCR) platform for the diagnosis of joint infections. This retrospective study evaluated its performance with synovial fluid and joint tissue specimens from native and prosthetic joints. Joint fluid and tissue specimens received from November 2023 to September 2024 were included. Some specimens were pooled prior to BJIP testing. BJIP results were compared to composite standard laboratory testing (culture, 16S rRNA gene PCR and sequencing [where performed], and concurrent microbiological results for that infection episode). Clinical data were collected (diagnosis, surgical history, antibiotic treatment). Subgroup analysis of native and prosthetic joint specimens was conducted. There were 224 specimens from 134 patients: 107 synovial fluids and 117 joint tissues. The most common organisms, reliably detected by the BJIP, were Staphylococcus aureus and Streptococcus spp. The on-panel positive percent agreement (PPA) was 90.5% for synovial fluids and 86.8% for joint tissues. There was no significant difference in the PPA for synovial fluids vs joint tissues, nor for native vs prosthetic joints. The additional diagnostic yield was one case. The absence of important pathogens in prosthetic joint infection, e.g., Cutibacterium acnes, reduces the overall PPA (74.5% synovial fluid, 77.6% joint tissue). The BJIP performs well in synovial fluid and joint tissue specimens, from native and prosthetic joints for on-panel organisms. There was minimal additional diagnostic yield. We suggest an implementation pathway using the BJIP upfront to provide rapid results to optimize patient care.IMPORTANCEThis study compared the BioFire Joint Infection polymerase chain reaction panel to standard culture-based methods for detecting organisms causing joint infections. This is a relatively new test, and the data published so far show it performs well with synovial fluid. There are limited published data using it for joint tissue specimens. We found that the BioFire Joint Infection Panel reliably detects pathogens in synovial fluid and joint tissue specimens, though its performance is limited by the absence of important pathogens associated with prosthetic joint infection, e.g., Cutibacterium acnes. We suggest using the panel to provide rapid diagnosis to optimize patient care.

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来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
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