叶黄素通过干扰DNA拓扑异构酶i α诱导乳腺癌细胞DNA损伤。

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2025-08-25 DOI:10.1080/10286020.2025.2544144

Yu-Yao Liu, Ya Chen, Qi-Qi Wang, Xi Zhang, Xiao-Xia Ma, Xing-De Wu, Jia Su

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引用次数: 0

摘要

DNA拓扑异构酶IIα (TOP2A)在DNA复制、转录和染色体分离过程中起着解决DNA拓扑胁迫的关键作用。叶黄素是脱氧鬼臼毒素的生物前体，具有抗肿瘤作用。本文探讨其在乳腺癌中的抗肿瘤作用。通过MTT、菌落形成、EdU实验和细胞周期分析，Yatein显著抑制乳腺癌细胞的增殖。此外，yatein抑制癌细胞的干性，抑制癌细胞的迁移和上皮-间质转化。机制研究表明，yatein抑制TOP2A活性，稳定TOP2A-DNA切割复合物，引起DNA损伤和细胞凋亡，提示其在乳腺癌治疗中的潜力。

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Yatein-induced DNA damage of breast cancer cells by interfering with DNA topoisomerase IIα.

DNA topoisomerase IIα (TOP2A) plays a key role in resolving DNA topological stress during replication, transcription, and chromosome segregation. Yatein, a biogenic precursor of deoxypodophyllotoxin possesses antitumor effects. Herein, we explored its antitumor effect in breast cancer. Yatein significantly inhibited the proliferation of breast cancer cells by MTT, colony formation, EdU assay, and cell cycle analysis. Moreover, yatein suppressed the stemness of cancer cells, causing inhibition of migration and epithelial-mesenchymal transition. Mechanism study revealed that yatein inhibited TOP2A activity and stabilized TOP2A-DNA cleavage complexes, caused DNA damage and apoptosis, suggesting its potential in the treatment of breast cancer.

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.