miR-484在妊娠期糖尿病患者中的表达趋势及调控作用

IF 1.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Yan Hou, Li Wan, Lijia Long
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引用次数: 0

摘要

目的:妊娠期糖尿病(GDM)以妊娠期糖代谢异常为特征。本研究旨在探讨miR-484在GDM中的表达模式及其功能意义。方法:通过RT-qPCR比较GDM患者与正常孕妇miR-484水平。随后,利用受试者工作特征(ROC)曲线评估miR-484对GDM的诊断和预测能力。下一阶段,我们将通过建立GDM小鼠模型,研究miR-484对GDM小鼠血糖水平和胰岛素抵抗的影响。最后,分离胰腺组织以阐明miR-484对炎症过程和氧化应激的影响。结果:在GDM患者中观察到miR-484水平升高,与血糖水平呈正相关。ROC曲线分析显示,miR-484对GDM具有较强的诊断和预测能力,AUC为0.835,敏感性为80.95%,特异性为82.00%。此外,在GDM模型中,miR-484的下调显著减轻了与GDM相关的不良反应,导致血糖水平和胰岛素抵抗的显著降低。在miR-484下调后,胰腺中IL-1β、TNF-α、IL-6和MDA水平也显著降低。同时,SOD和CAT水平显著升高。结论:miR-484可能作为GDM早期识别的生物标志物。下调这一分子有利于降低血糖、胰岛素抵抗和胰腺炎症或氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The expression trend and regulatory role of miR-484 in patients with gestational diabetes mellitus.

Objective: Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.

Methods: The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR. Subsequently, the diagnostic and predictive capacity of miR-484 for GDM was assessed utilizing Receiver Operating Characteristic (ROC) curve. In the next phase, the effects of miR-484 on blood glucose levels and insulin resistance in GDM mice were studied by establishing a GDM mouse model. Finally, pancreatic tissues were isolated to elucidate the influence of miR-484 on inflammation processes and oxidative stress.

Results: Elevated levels of miR-484 have been observed in GDM patients, demonstrating a positive correlation with blood glucose levels. ROC curve analysis indicated that miR-484 possesses substantial diagnostic and predictive capabilities for GDM, with an AUC of 0.835, a sensitivity of 80.95%, and a specificity of 82.00%. Furthermore, in GDM models, the downregulation of miR-484 significantly mitigated the adverse effects associated with GDM, leading to notable reductions in blood glucose levels and insulin resistance. Following the down-regulation of miR-484, a notable reduction in the levels of IL-1β, TNF-α, IL-6, and MDA in the pancreas was also observed. Concurrently, there was a significant increase in the levels of SOD and CAT.

Conclusion: miR-484 might act as a biomarker for the early-stage recognition of GDM. Down-regulating this molecule is beneficial for lowering blood glucose, insulin resistance, and pancreatic inflammation or oxidative stress.

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来源期刊
Irish Journal of Medical Science
Irish Journal of Medical Science 医学-医学:内科
CiteScore
3.70
自引率
4.80%
发文量
357
审稿时长
4-8 weeks
期刊介绍: The Irish Journal of Medical Science is the official organ of the Royal Academy of Medicine in Ireland. Established in 1832, this quarterly journal is a contribution to medical science and an ideal forum for the younger medical/scientific professional to enter world literature and an ideal launching platform now, as in the past, for many a young research worker. The primary role of both the Academy and IJMS is that of providing a forum for the exchange of scientific information and to promote academic discussion, so essential to scientific progress.
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