{"title":"miR-484在妊娠期糖尿病患者中的表达趋势及调控作用","authors":"Yan Hou, Li Wan, Lijia Long","doi":"10.1007/s11845-025-04043-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.</p><p><strong>Methods: </strong>The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR. Subsequently, the diagnostic and predictive capacity of miR-484 for GDM was assessed utilizing Receiver Operating Characteristic (ROC) curve. In the next phase, the effects of miR-484 on blood glucose levels and insulin resistance in GDM mice were studied by establishing a GDM mouse model. Finally, pancreatic tissues were isolated to elucidate the influence of miR-484 on inflammation processes and oxidative stress.</p><p><strong>Results: </strong>Elevated levels of miR-484 have been observed in GDM patients, demonstrating a positive correlation with blood glucose levels. ROC curve analysis indicated that miR-484 possesses substantial diagnostic and predictive capabilities for GDM, with an AUC of 0.835, a sensitivity of 80.95%, and a specificity of 82.00%. Furthermore, in GDM models, the downregulation of miR-484 significantly mitigated the adverse effects associated with GDM, leading to notable reductions in blood glucose levels and insulin resistance. Following the down-regulation of miR-484, a notable reduction in the levels of IL-1β, TNF-α, IL-6, and MDA in the pancreas was also observed. Concurrently, there was a significant increase in the levels of SOD and CAT.</p><p><strong>Conclusion: </strong>miR-484 might act as a biomarker for the early-stage recognition of GDM. Down-regulating this molecule is beneficial for lowering blood glucose, insulin resistance, and pancreatic inflammation or oxidative stress.</p>","PeriodicalId":14507,"journal":{"name":"Irish Journal of Medical Science","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The expression trend and regulatory role of miR-484 in patients with gestational diabetes mellitus.\",\"authors\":\"Yan Hou, Li Wan, Lijia Long\",\"doi\":\"10.1007/s11845-025-04043-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.</p><p><strong>Methods: </strong>The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR. Subsequently, the diagnostic and predictive capacity of miR-484 for GDM was assessed utilizing Receiver Operating Characteristic (ROC) curve. In the next phase, the effects of miR-484 on blood glucose levels and insulin resistance in GDM mice were studied by establishing a GDM mouse model. Finally, pancreatic tissues were isolated to elucidate the influence of miR-484 on inflammation processes and oxidative stress.</p><p><strong>Results: </strong>Elevated levels of miR-484 have been observed in GDM patients, demonstrating a positive correlation with blood glucose levels. ROC curve analysis indicated that miR-484 possesses substantial diagnostic and predictive capabilities for GDM, with an AUC of 0.835, a sensitivity of 80.95%, and a specificity of 82.00%. Furthermore, in GDM models, the downregulation of miR-484 significantly mitigated the adverse effects associated with GDM, leading to notable reductions in blood glucose levels and insulin resistance. Following the down-regulation of miR-484, a notable reduction in the levels of IL-1β, TNF-α, IL-6, and MDA in the pancreas was also observed. Concurrently, there was a significant increase in the levels of SOD and CAT.</p><p><strong>Conclusion: </strong>miR-484 might act as a biomarker for the early-stage recognition of GDM. Down-regulating this molecule is beneficial for lowering blood glucose, insulin resistance, and pancreatic inflammation or oxidative stress.</p>\",\"PeriodicalId\":14507,\"journal\":{\"name\":\"Irish Journal of Medical Science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Irish Journal of Medical Science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s11845-025-04043-x\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Irish Journal of Medical Science","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11845-025-04043-x","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
The expression trend and regulatory role of miR-484 in patients with gestational diabetes mellitus.
Objective: Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.
Methods: The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR. Subsequently, the diagnostic and predictive capacity of miR-484 for GDM was assessed utilizing Receiver Operating Characteristic (ROC) curve. In the next phase, the effects of miR-484 on blood glucose levels and insulin resistance in GDM mice were studied by establishing a GDM mouse model. Finally, pancreatic tissues were isolated to elucidate the influence of miR-484 on inflammation processes and oxidative stress.
Results: Elevated levels of miR-484 have been observed in GDM patients, demonstrating a positive correlation with blood glucose levels. ROC curve analysis indicated that miR-484 possesses substantial diagnostic and predictive capabilities for GDM, with an AUC of 0.835, a sensitivity of 80.95%, and a specificity of 82.00%. Furthermore, in GDM models, the downregulation of miR-484 significantly mitigated the adverse effects associated with GDM, leading to notable reductions in blood glucose levels and insulin resistance. Following the down-regulation of miR-484, a notable reduction in the levels of IL-1β, TNF-α, IL-6, and MDA in the pancreas was also observed. Concurrently, there was a significant increase in the levels of SOD and CAT.
Conclusion: miR-484 might act as a biomarker for the early-stage recognition of GDM. Down-regulating this molecule is beneficial for lowering blood glucose, insulin resistance, and pancreatic inflammation or oxidative stress.
期刊介绍:
The Irish Journal of Medical Science is the official organ of the Royal Academy of Medicine in Ireland. Established in 1832, this quarterly journal is a contribution to medical science and an ideal forum for the younger medical/scientific professional to enter world literature and an ideal launching platform now, as in the past, for many a young research worker.
The primary role of both the Academy and IJMS is that of providing a forum for the exchange of scientific information and to promote academic discussion, so essential to scientific progress.