{"title":"哥伦比亚白色念珠菌种复合体外阴阴道分离株HWP1基因序列多样性和抗真菌敏感性模式","authors":"Soraya Morales-López, Yeneiris Villero Wolf, Yulibeth Torres, Deyner Lechuga, Luis Caicedo, Guillermo García-Effron","doi":"10.1155/ijm/8507361","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background:</b> <i>Candida albicans</i> complex species are the main cause of candidiasis. <b>Objectives:</b> The purpose of this study was to report the prevalence and genetic diversity of <i>C. albicans</i> complex using hyphal wall protein 1 (HWP1) gene size polymorphism, as well as the susceptibility patterns to fluconazole and voriconazole. <b>Methods:</b> A total of 170 yeast isolates were obtained from vulvovaginal samples, and phenotypic and proteomic identification was performed. <b>Results:</b> Most clinical isolates were <i>C. albicans</i> complex (<i>n</i> = 153) followed by <i>C. glabrata</i> (<i>n</i> = 13), <i>C. parapsilosis</i> complex (<i>n</i> = 2), and <i>Pichia kudriavzevii</i> (<i>n</i> = 2). Among <i>C. albicans</i> complexes, all isolates were <i>C. albicans sensu stricto</i> and 2.61% and 4.58% were resistant to fluconazole and voriconazole, respectively. <b>Conclusions:</b> The presence of different alleles was confirmed, heterozygosity was more common than homozygosity (71.03% vs. 28.97%), and some isolates showed a homozygosis pattern not previously described. Despite these genetic diversities, no specific genotype was linked to azole resistance.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"8507361"},"PeriodicalIF":3.2000,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393925/pdf/","citationCount":"0","resultStr":"{\"title\":\"HWP1 Gene Sequence Diversity and Antifungal Susceptibility Patterns in Colombian Vulvovaginal Isolates of the <i>Candida albicans</i> Species Complex.\",\"authors\":\"Soraya Morales-López, Yeneiris Villero Wolf, Yulibeth Torres, Deyner Lechuga, Luis Caicedo, Guillermo García-Effron\",\"doi\":\"10.1155/ijm/8507361\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Background:</b> <i>Candida albicans</i> complex species are the main cause of candidiasis. <b>Objectives:</b> The purpose of this study was to report the prevalence and genetic diversity of <i>C. albicans</i> complex using hyphal wall protein 1 (HWP1) gene size polymorphism, as well as the susceptibility patterns to fluconazole and voriconazole. <b>Methods:</b> A total of 170 yeast isolates were obtained from vulvovaginal samples, and phenotypic and proteomic identification was performed. <b>Results:</b> Most clinical isolates were <i>C. albicans</i> complex (<i>n</i> = 153) followed by <i>C. glabrata</i> (<i>n</i> = 13), <i>C. parapsilosis</i> complex (<i>n</i> = 2), and <i>Pichia kudriavzevii</i> (<i>n</i> = 2). Among <i>C. albicans</i> complexes, all isolates were <i>C. albicans sensu stricto</i> and 2.61% and 4.58% were resistant to fluconazole and voriconazole, respectively. <b>Conclusions:</b> The presence of different alleles was confirmed, heterozygosity was more common than homozygosity (71.03% vs. 28.97%), and some isolates showed a homozygosis pattern not previously described. Despite these genetic diversities, no specific genotype was linked to azole resistance.</p>\",\"PeriodicalId\":14098,\"journal\":{\"name\":\"International Journal of Microbiology\",\"volume\":\"2025 \",\"pages\":\"8507361\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393925/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/ijm/8507361\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/ijm/8507361","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
HWP1 Gene Sequence Diversity and Antifungal Susceptibility Patterns in Colombian Vulvovaginal Isolates of the Candida albicans Species Complex.
Background:Candida albicans complex species are the main cause of candidiasis. Objectives: The purpose of this study was to report the prevalence and genetic diversity of C. albicans complex using hyphal wall protein 1 (HWP1) gene size polymorphism, as well as the susceptibility patterns to fluconazole and voriconazole. Methods: A total of 170 yeast isolates were obtained from vulvovaginal samples, and phenotypic and proteomic identification was performed. Results: Most clinical isolates were C. albicans complex (n = 153) followed by C. glabrata (n = 13), C. parapsilosis complex (n = 2), and Pichia kudriavzevii (n = 2). Among C. albicans complexes, all isolates were C. albicans sensu stricto and 2.61% and 4.58% were resistant to fluconazole and voriconazole, respectively. Conclusions: The presence of different alleles was confirmed, heterozygosity was more common than homozygosity (71.03% vs. 28.97%), and some isolates showed a homozygosis pattern not previously described. Despite these genetic diversities, no specific genotype was linked to azole resistance.
期刊介绍:
International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.