肺炎克雷伯菌临床分离株抗微生物药物耐药性模式、VI型分泌系统和毒力因素的鉴定:一项横断面研究。

IF 3.2 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI:10.1155/ijm/9634257
Mohammad Hossein Haddadi, Elahe Taki, Abbas Maleki, Jamshid Mashhadi, Fatemeh Shahi, Maryam Koupaei, Nourkhoda Sadeghifard, Reza Faraji, Melika Moradi, Hassan Valadbeigi, Saeed Khoshnood
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引用次数: 0

摘要

背景:肺炎克雷伯菌是一种与一系列严重感染相关的机会性病原体。它分为典型肺炎克雷伯菌(cKp)和高毒力肺炎克雷伯菌(hvKp)两种,其中hvKp表现出更强的毒力。本研究检测了cKp和hvKp菌株中VI型分泌系统(T6SS)的存在和毒力因子,同时评估了抗微生物药物耐药性。方法:2023年6 - 12月在伊朗伊拉姆市住院患者中采集83株肺炎克雷伯菌分离株。采用纸片扩散法进行药敏试验。采用串珠试验、碲酸盐耐药性、聚合酶链反应(PCR)检测peg-344、iucA和iutA的存在,对高致病性肺炎克雷伯菌分离株进行鉴定。此外,PCR检测毒力、β-内酰胺酶和T6SS基因。结果:83株分离株中hvKp阳性率为16.87%,cKp阳性率为83.13%。所有菌株均为多药耐药(MDR),其中18.07%对碳青霉烯类耐药。除了环丙沙星、四环素和阿奇霉素外,hvKp和cKp的抗生素耐药模式无显著差异。除环丙沙星外,t6ss阳性与t6ss阴性菌株间无显著性差异。除环丙沙星耐药外,T6SS的存在与毒力或耐药基因无显著相关性(p = 0.0219)。结论:监测、感染控制和靶向治疗对对抗高毒力和耐药肺炎克雷伯菌至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of Antimicrobial Resistance Patterns, Type VI Secretion System, and Virulence Factors in Clinical Isolates of Klebsiella pneumoniae: A Cross-Sectional Study.

Background: Klebsiella pneumoniae is an opportunistic pathogen linked to a range of severe infections. It is classified into classic K. pneumoniae (cKp) and hypervirulent K. pneumoniae (hvKp) forms, with hvKp showing enhanced virulence. This study examines Type VI secretion system (T6SS) presence and virulence factors in cKp and hvKp strains while assessing antimicrobial resistance. Methods: Eighty-three K. pneumoniae isolates were collected from hospitalized patients in Ilam, Iran, between June and December 2023. Antimicrobial susceptibility testing was conducted using the disk diffusion method. Hypervirulent K. pneumoniae isolates were identified by string test, tellurite resistance, and presence of peg-344, iucA, and iutA using polymerase chain reaction (PCR). Furthermore, PCR detected virulence, β-lactamases, and T6SS genes. Results: Of 83 isolates, 16.87% and 83.13% were hvKp and cKp, respectively. All isolates were multidrug-resistant (MDR), with 18.07% exhibiting carbapenem resistance. The pattern of antibiotic resistance in hvKp and cKp is not significantly different, except for ciprofloxacin, tetracycline, and azithromycin. Also, there was no significant difference between T6SS-positive and T6SS-negative isolates except for ciprofloxacin. T6SS presence did not significantly correlate with virulence or resistance genes, except for ciprofloxacin resistance (p = 0.0219). Conclusion: Surveillance, infection control, and targeted therapies are essential to combat hypervirulent and drug-resistant K. pneumoniae.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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