尼日利亚零售肉类中粪肠球菌的基因组学和生物信息学研究。

IF 3.2 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2025-08-20 eCollection Date: 2025-01-01 DOI:10.1155/ijm/7325430
Charles Ayodeji Osunla, Ayorinde Akinbobola, Arif Elshafea, Esther Eyram Asare Yeboah, Olayemi Stephen Bakare, Aderonke Fayanju, Dorcas Oladayo Fatoba, Bright Boamah, Daniel Gyamfi Amoako
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引用次数: 0

摘要

粪肠球菌是一种共生和机会性病原体,因其抗微生物药物耐药性和人畜共患的潜力而日益受到重视。本研究采用全基因组测序(WGS)对零售肉类样本中的粪肠杆菌分离物进行了表征,重点研究了抗微生物药物耐药性基因(ARGs)、毒力决定因素、可移动遗传元件和系统基因组关系。从尼日利亚Akungba-Akoko的零售市场收集了50份生肉样本,包括鸡肉(n = 18)、牛肉(n = 17)和火鸡(n = 15)。确认的分离株进行了抗菌药敏试验和基于wgs的基因组分析。分离出10株粪肠球菌,主要来自鸡。所有患者均表现出对克林霉素、红霉素和四环素的耐药性。AMR的优势基因包括aac(6)-aph(2″)、ant(6)-Ia、lsa(A)、erm(B)、tet(M)和tet(L)。质粒复制子rep9c和repUS43分别与序列类型ST477和ST16相关。MGEs如IS3、IS6、IS256和IS1380与抗性和毒力基因共定位。系统基因组学分析显示了两个主要谱系(ST477和ST16),并显示了非洲分离株的地理聚类。由于存在水平基因转移和人畜共患传播的风险,食源性粪肠杆菌中多药耐药、毒力因子和MGEs的共存引起了公共卫生问题。这些发现支持在粮食系统中加强基因组监测和抗菌素耐药性控制战略的必要性,特别是在低收入和中等收入国家。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genomic and Bioinformatic Insights Into <i>Enterococcus faecalis</i> From Retail Meats in Nigeria.

Genomic and Bioinformatic Insights Into <i>Enterococcus faecalis</i> From Retail Meats in Nigeria.

Genomic and Bioinformatic Insights Into <i>Enterococcus faecalis</i> From Retail Meats in Nigeria.

Genomic and Bioinformatic Insights Into Enterococcus faecalis From Retail Meats in Nigeria.

Enterococcus faecalis is a commensal and opportunistic pathogen increasingly recognized for its antimicrobial resistance (AMR) and zoonotic potential. This study employs whole-genome sequencing (WGS) to characterize E. faecalis isolates from retail meat samples, focusing on antimicrobial resistance genes (ARGs), virulence determinants, mobile genetic elements, and phylogenomic relationships. Fifty raw meat samples, including chicken (n = 18), beef (n = 17), and turkey (n = 15), were collected from retail markets in Akungba-Akoko, Nigeria. Confirmed isolates underwent antimicrobial susceptibility testing and WGS-based genomic analysis. Ten E. faecalis isolates were recovered, predominantly from chicken. All exhibited resistance to clindamycin, erythromycin, and tetracycline. Dominant AMR genes included aac(6⁣')-aph(2), ant(6)-Ia, lsa(A), erm(B), tet(M), and tet(L). Plasmid replicons rep9c and repUS43 were associated with sequence types ST477 and ST16, respectively. MGEs such as IS3, IS6, IS256, and IS1380 colocalized with resistance and virulence genes. Phylogenomic analysis revealed two major lineages (ST477 and ST16) and indicated geographic clustering across African isolates. The co-occurrence of multidrug resistance, virulence factors, and MGEs in foodborne E. faecalis poses a public health concern due to the risk of horizontal gene transfer and zoonotic spread. These findings support the need for strengthened genomic surveillance and AMR control strategies in food systems, particularly within low- and middle-income countries.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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