伊朗东北部Gonabad Bohlool医院不同病房棘阿米巴基因分型研究

IF 3.2 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI:10.1155/ijm/8672955
Mitra Salehi, Adel Spotin, Mina Moradi, Morteza Rostamian, Hassan Reza Rokni
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引用次数: 0

摘要

背景:棘阿米巴是一种自由生活的变形虫,广泛存在于自然界的土壤、水和灰尘等不同环境中。这种寄生虫是阿米巴角膜炎和肉芽肿性阿米巴脑炎的病因。本研究旨在调查伊朗东北部Gonabad Bohlool医院不同病房棘阿米巴原虫的流行情况和基因型。方法:2023年在戈纳巴德医院各病房设备中采集样本183份;拭子在非营养性琼脂(NNA)培养基中培养,加入杀灭的大肠杆菌。在含有星状囊的阳性样品中,采用JDP1和JDP2引物进行PCR分子检测。为了确认棘阿米巴原虫,还进行了基因分型和测序。结果:183份样本中检出棘阿米巴原虫114种(62.30%);棘阿米巴污染比例最高的是急诊病房,为81.82%,最低的是手术和影像室,为50%。工作区域和设备棘阿米巴污染比例最高(66.67%),医疗设备棘阿米巴污染比例最低(56.14%)。本研究还确定了T4 (n = 10, 43.5%)、T3 (n = 4, 17.4%)、T5 (n = 4, 17.4%)、T11 (n = 3, 13%)、T2 (n = 2, 8.7%)基因型。结论:本研究结果表明,棘阿米巴在急诊病房和工作人员区域的表面和设备上更为常见。考虑到这种阿米巴原虫引起的危险并发症,建议开展卫生教育以提高对传播领域的认识,并采取卫生措施防止污染,包括消毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genotyping of <i>Acanthamoeba</i> From Different Wards of Gonabad Bohlool Hospital, Northeastern Iran.

Genotyping of <i>Acanthamoeba</i> From Different Wards of Gonabad Bohlool Hospital, Northeastern Iran.

Genotyping of <i>Acanthamoeba</i> From Different Wards of Gonabad Bohlool Hospital, Northeastern Iran.

Genotyping of Acanthamoeba From Different Wards of Gonabad Bohlool Hospital, Northeastern Iran.

Background: Acanthamoeba is a free-living amoeba that is widely found in nature in different environments such as soil, water, and dust. This parasite is the cause of amoebic keratitis and granulomatous amoebic encephalitis. This study is aimed at investigating the prevalence and genotypes of Acanthamoeba in different wards of Gonabad Bohlool Hospital, northeastern Iran. Methods: One hundred and eighty-three samples were collected from equipment in various wards of Gonabad hospital in 2023; swabs were cultured in nonnutrient agar (NNA) medium with the addition of killed Escherichia coli. In positive samples containing stellate cysts, PCR molecular testing was performed using JDP1 and JDP2 primers. To confirm Acanthamoeba, genotyping and sequencing were also done. Results: Acanthamoeba sp. was observed in 114 out of 183 samples (62.30%). The highest percentage of Acanthamoeba contamination was in the emergency ward with 81.82%, and the lowest percentage was in the operating and imaging room with 50%. Moreover, the highest percentage of Acanthamoeba contamination was in staff areas and equipment with 66.67%, and the lowest percentage was 56.14% on medical equipment. Also, in this research, the Genotypes T4 (n = 10, 43.5%), T3 (n = 4, 17.4%), T5 (n = 4, 17.4%), T11 (n = 3, 13%), and T2 (n = 2, 8.7%) were determined. Conclusions: The findings of this study showed that Acanthamoeba is more common in the emergency ward and on the surfaces and equipment of the staff areas. Considering the dangerous complications caused by this amoeba, health education to increase awareness in the field of transmission as well as health measures to prevent contamination, including disinfection, is recommended.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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