含有blaKPC和mcr的阴沟肠杆菌复合体的分子流行病学和基因组学研究：对耐药基因传播的影响。

IF 4.6 2区医学 Q1 INFECTIOUS DISEASES

International Journal of Antimicrobial Agents Pub Date : 2025-08-28 DOI:10.1016/j.ijantimicag.2025.107602

Boqian Wang , Jingjing Fu , Yaqing He , Kexin Li , Guifang Hu , Mingda Hu , Xin Wang , Wanqiu Liu , Rui Zhang , Yuxin Wang , Buaijier Aimaiti , Xinru Zhao , Hongbin Song , Xiaofeng Hu , Hongguang Ren

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引用次数: 0

摘要

目的：系统研究全球携带blaKPC和mcr以及耐药基因共存的阴沟肠杆菌（Enterobacter cloacae complex， ECC）的分子流行病学和基因组特征。目的是为监测临床耐药菌株和超耐药质粒提供见解和建议。方法：利用NCBI GenBank数据库2003-2024年全基因组测序数据，对281株同时携带blaKPC和mcr基因的ECC分离株进行分析。我们构建了ECC菌株的系统发育树进行系统发育分析。利用ABRicate和CARD数据库鉴定耐药基因，并对其分布进行分析。利用PlasmidFinder分析blaKPC和mcr的质粒复制子类型，包括这些基因的上游和下游遗传环境。结果：同时携带blaKPC和mcr的优势基因型组合为blaKPC-3和mcr-9.1，其次为blaKPC-4和mcr-9.1，优势菌株为荷马切伊杆菌。xiangfangensis。携带blaKPC和mcr-9的IncHI2（2A）质粒主要在美国的基因组中检测到。系统发育分析表明，ECC菌株的blaKPC-mcr-9-IncHI2（2A）质粒与mcr-9-IncHI2（2A）具有较高的遗传相似性，表明后者可能获得了额外的高度可转移的blaKPC。结论：ECC菌株已成为blaKPC和mcr-9的重要储集群，IncHI2（2A）质粒是碳青霉烯类和粘菌素耐药基因水平共传播的潜在载体。应实施有效的监测，以评估单个ECC分离株甚至单个质粒中blaKPC和mcr-9共藏的流行情况。

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Molecular epidemiology and genomic insights into Enterobacter cloacae complex co-harbouring blaKPC and mcr: Implications for resistance gene transmission

Objective

This study aims to systematically investigate the molecular epidemiology and genomic characteristics of Enterobacter cloacae complex (ECC) strains globally harbouring bla_KPC and mcr, as well as the co-existence of drug resistance genes. The goal is to provide insights and recommendations for monitoring clinical drug-resistant strains and super-resistant plasmids.

Methods

This study analysed 281 ECC isolates harbouring both bla_KPC and mcr, obtained from NCBI GenBank database (2003-2024) with whole-genome sequencing data. We constructed a phylogenetic tree of ECC strains for phylogenetic analysis. Resistance genes were identified using the ABRicate and CARD databases, and their distribution was examined. Plasmid replicon types for bla_KPC and mcr were analysed with PlasmidFinder, including upstream and downstream genetic environments of these genes.

Results

The predominant genotype combinations harbouring both bla_KPC and mcr are bla_KPC-3 and mcr-9.1, followed by bla_KPC-4 and mcr-9.1, with the dominant strain being E. hormaechei ssp. xiangfangensis. The IncHI2(2A) plasmid co-harbouring bla_KPC and mcr-9 was mainly detected in genomes from the United States. Phylogenetic analysis indicated that the bla_KPC-mcr-9-IncHI2(2A) plasmids in ECC strains have a high genetic similarity to mcr-9-IncHI2(2A), suggesting that the latter may have acquired the additional highly transferable bla_KPC.

Conclusions

ECC strains have become an important reservoir cluster for bla_KPC and mcr-9, and the IncHI2(2A) plasmid is a potential vector for the horizontal co-transmission of carbapenem and colistin resistance genes. Effective monitoring should be implemented to assess the prevalence of co-harbouring bla_KPC and mcr-9 in individual ECC isolates and even in single plasmid.

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来源期刊

International Journal of Antimicrobial Agents 医学-传染病学

CiteScore

21.60

自引率

0.90%

发文量

176

审稿时长

36 days

期刊介绍： The International Journal of Antimicrobial Agents is a peer-reviewed publication offering comprehensive and current reference information on the physical, pharmacological, in vitro, and clinical properties of individual antimicrobial agents, covering antiviral, antiparasitic, antibacterial, and antifungal agents. The journal not only communicates new trends and developments through authoritative review articles but also addresses the critical issue of antimicrobial resistance, both in hospital and community settings. Published content includes solicited reviews by leading experts and high-quality original research papers in the specified fields.