硒蛋白GPX3抑制胃腺癌增殖,提高患者生存率。

IF 2.9 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chunfeng Zhang, Lijuan Ma, Ying Shao, Shanpeng Cui, Li Li
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引用次数: 0

摘要

胃腺癌(STAD)是一种在世界范围内高度流行和致死性的恶性肿瘤,其发生和发展受多种因素的调控。近年来,硒蛋白谷胱甘肽过氧化物酶3 (GPX3)因其抗氧化特性和在多种癌症细胞氧化应激调节中的作用而受到广泛关注。我们的研究深入研究了GPX3在STAD中的表达,并研究了其对肿瘤细胞生长的影响,为其潜在的抗肿瘤机制提供了新的见解。分析来自TCGA数据库的STAD组织中GPX3的表达水平,并与正常胃组织中的GPX3表达水平进行对比。比较GPX3在STAD组织和正常胃组织中的表达水平,并通过生存分析评估其与患者预后的相关性。此外,我们利用定量PCR (qPCR)和CCK-8增殖试验验证了GPX3在STAD细胞系(MNK-45、MGC-803、N87和HGC-27)中的表达变化及其对肿瘤细胞生长的影响。我们的研究结果表明,与正常胃组织相比,GPX3在STAD组织中的表达明显下调。生存分析进一步显示,GPX3高表达的患者表现出更好的长期生存率,提示其具有潜在的肿瘤抑制功能。体外实验证实GPX3在STAD细胞系中可有效下调或过表达。CCK-8增殖实验表明,GPX3过表达显著抑制肿瘤细胞增殖,而GPX3敲低促进细胞生长。本研究为GPX3作为STAD的潜在治疗靶点提供了新的实验证据,为未来STAD的分子靶向治疗提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Selenoprotein GPX3 suppresses gastric adenocarcinoma proliferation and improves patient survival.

Stomach adenocarcinoma (STAD) is a highly prevalent and lethal malignancy worldwide, with its occurrence and progression regulated by multiple factors. In recent years, selenoprotein glutathione peroxidase 3 (GPX3) has gained significant attention due to its antioxidant properties and role in cellular oxidative stress regulation across various cancers. Our study delved into the expression of GPX3 in STAD and investigated its impact on tumor cell growth, providing insights into its potential anti-tumor mechanisms. The expression levels of GPX3 were analyzed in STAD tissues sourced from the TCGA database and contrasted with the levels found in normal gastric tissues. The expression levels of GPX3 were contrasted between STAD tissues and normal gastric tissues, and their correlation with patient prognosis was assessed by survival analysis. Additionally, we validated GPX3 expression changes and its effects on tumor cell growth using quantitative PCR (qPCR) and CCK-8 proliferation assays in STAD cell lines (MNK-45, MGC-803, N87, and HGC-27). Our findings suggest that GPX3 expression is significantly downregulated in STAD tissues compared to normal gastric tissues. Survival analysis further reveals that patients with high GPX3 expression exhibit better long-term survival rates, suggesting a potential tumor-suppressive function. In vitro experiments confirmed effective knockdown or overexpression of GPX3 in STAD cell lines. CCK-8 proliferation assays demonstrated that GPX3 overexpression significantly inhibited tumor cell proliferation, whereas GPX3 knockdown promoted cell growth. This study provides new experimental evidence supporting GPX3 as a potential therapeutic target for STAD and offers a theoretical foundation for future molecular-targeted therapies for STAD.

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来源期刊
Free Radical Research
Free Radical Research 生物-生化与分子生物学
CiteScore
6.70
自引率
0.00%
发文量
47
审稿时长
3 months
期刊介绍: Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.
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