{"title":"揭示了胡柴胡在皮肤病学应用中的抗菌、生物膜抑制和光保护潜力。","authors":"Mirjana Milinkovic Sreckovic, Jovana Petrovic, Marija Ivanov, Uros Gasic, Milena Milivojevic, Danijela Stanisavljevic Ninkovic, Dejan Stojkovic","doi":"10.17179/excli2025-8344","DOIUrl":null,"url":null,"abstract":"<p><p><i>Bupleurum falcatum</i> L. is known for its therapeutic properties, especially in treating fever, inflammation, and infectious diseases. However, its potential for dermatological applications remains mainly unexplored. Thus, the present study explores antimicrobial potential of <i>B. falcatum</i> against biofilm-associated infections, antibiotic-resistant strains, and UV-induced skin damage. This aligns with the growing interest in natural products as sources of bioactive compounds with skin-protecting features. Herein, we employed maceration (M) and ultrasound-assisted extraction (USA) at 50 Hz and 100 Hz (USA 50 and USA 100) to obtain extracts from aerial parts of the plant. Chemical profiling was performed using UHPLC. Antimicrobial activity, biofilm inhibition, EPS and eDNA production were assessed using microdilution test, crystal violet, Congo red, and eDNA assays, respectively. Cytotoxicity and photoprotective effects were evaluated on human keratinocytes using the MTT assay. Chemical analysis identified 64 compounds, including benzoic and cinnamic acid derivatives, flavonoid glycosides, and saikosaponins. Extracts showed strong antimicrobial activity against <i>Pseudomonas aeruginosa</i> and methicillin-resistant <i>Staphylococcus aureus</i> (MIC as low as 0.5 mg/mL). The M extract displayed moderate biofilm inhibition and reduced eDNA production. Cytotoxicity assays confirmed safety on keratinocytes, while M and USA 100 extracts demonstrated photoprotective effects. <i>B. falcatum</i> extracts showed promising potential in addressing biofilm-associated infections, antibiotic resistance, and UV-induced skin damage. See also the graphical abstract(Fig. 1).</p>","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"24 ","pages":"779-796"},"PeriodicalIF":4.9000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381368/pdf/","citationCount":"0","resultStr":"{\"title\":\"Unveiling the antimicrobial, biofilm inhibition, and photoprotective potential of Bupleurum falcatum L. for dermatological applications.\",\"authors\":\"Mirjana Milinkovic Sreckovic, Jovana Petrovic, Marija Ivanov, Uros Gasic, Milena Milivojevic, Danijela Stanisavljevic Ninkovic, Dejan Stojkovic\",\"doi\":\"10.17179/excli2025-8344\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Bupleurum falcatum</i> L. is known for its therapeutic properties, especially in treating fever, inflammation, and infectious diseases. However, its potential for dermatological applications remains mainly unexplored. Thus, the present study explores antimicrobial potential of <i>B. falcatum</i> against biofilm-associated infections, antibiotic-resistant strains, and UV-induced skin damage. This aligns with the growing interest in natural products as sources of bioactive compounds with skin-protecting features. Herein, we employed maceration (M) and ultrasound-assisted extraction (USA) at 50 Hz and 100 Hz (USA 50 and USA 100) to obtain extracts from aerial parts of the plant. Chemical profiling was performed using UHPLC. Antimicrobial activity, biofilm inhibition, EPS and eDNA production were assessed using microdilution test, crystal violet, Congo red, and eDNA assays, respectively. Cytotoxicity and photoprotective effects were evaluated on human keratinocytes using the MTT assay. Chemical analysis identified 64 compounds, including benzoic and cinnamic acid derivatives, flavonoid glycosides, and saikosaponins. Extracts showed strong antimicrobial activity against <i>Pseudomonas aeruginosa</i> and methicillin-resistant <i>Staphylococcus aureus</i> (MIC as low as 0.5 mg/mL). The M extract displayed moderate biofilm inhibition and reduced eDNA production. Cytotoxicity assays confirmed safety on keratinocytes, while M and USA 100 extracts demonstrated photoprotective effects. <i>B. falcatum</i> extracts showed promising potential in addressing biofilm-associated infections, antibiotic resistance, and UV-induced skin damage. 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Unveiling the antimicrobial, biofilm inhibition, and photoprotective potential of Bupleurum falcatum L. for dermatological applications.
Bupleurum falcatum L. is known for its therapeutic properties, especially in treating fever, inflammation, and infectious diseases. However, its potential for dermatological applications remains mainly unexplored. Thus, the present study explores antimicrobial potential of B. falcatum against biofilm-associated infections, antibiotic-resistant strains, and UV-induced skin damage. This aligns with the growing interest in natural products as sources of bioactive compounds with skin-protecting features. Herein, we employed maceration (M) and ultrasound-assisted extraction (USA) at 50 Hz and 100 Hz (USA 50 and USA 100) to obtain extracts from aerial parts of the plant. Chemical profiling was performed using UHPLC. Antimicrobial activity, biofilm inhibition, EPS and eDNA production were assessed using microdilution test, crystal violet, Congo red, and eDNA assays, respectively. Cytotoxicity and photoprotective effects were evaluated on human keratinocytes using the MTT assay. Chemical analysis identified 64 compounds, including benzoic and cinnamic acid derivatives, flavonoid glycosides, and saikosaponins. Extracts showed strong antimicrobial activity against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MIC as low as 0.5 mg/mL). The M extract displayed moderate biofilm inhibition and reduced eDNA production. Cytotoxicity assays confirmed safety on keratinocytes, while M and USA 100 extracts demonstrated photoprotective effects. B. falcatum extracts showed promising potential in addressing biofilm-associated infections, antibiotic resistance, and UV-induced skin damage. See also the graphical abstract(Fig. 1).
期刊介绍:
EXCLI Journal publishes original research reports, authoritative reviews and case reports of experimental and clinical sciences.
The journal is particularly keen to keep a broad view of science and technology, and therefore welcomes papers which bridge disciplines and may not suit the narrow specialism of other journals. Although the general emphasis is on biological sciences, studies from the following fields are explicitly encouraged (alphabetical order):
aging research, behavioral sciences, biochemistry, cell biology, chemistry including analytical chemistry, clinical and preclinical studies, drug development, environmental health, ergonomics, forensic medicine, genetics, hepatology and gastroenterology, immunology, neurosciences, occupational medicine, oncology and cancer research, pharmacology, proteomics, psychiatric research, psychology, systems biology, toxicology