揭示了胡柴胡在皮肤病学应用中的抗菌、生物膜抑制和光保护潜力。

IF 4.9 3区 生物学 Q1 BIOLOGY
EXCLI Journal Pub Date : 2025-07-11 eCollection Date: 2025-01-01 DOI:10.17179/excli2025-8344
Mirjana Milinkovic Sreckovic, Jovana Petrovic, Marija Ivanov, Uros Gasic, Milena Milivojevic, Danijela Stanisavljevic Ninkovic, Dejan Stojkovic
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引用次数: 0

摘要

柴胡以其治疗特性而闻名,特别是在治疗发烧、炎症和传染病方面。然而,其在皮肤病学上的应用潜力仍未得到充分开发。因此,本研究探讨了镰状芽孢杆菌对生物膜相关感染、抗生素耐药菌株和紫外线诱导的皮肤损伤的抗菌潜力。这与人们对天然产品作为具有皮肤保护功能的生物活性化合物的来源日益增长的兴趣相一致。在此,我们采用浸渍(M)和超声辅助提取(USA)在50 Hz和100 Hz (USA 50和USA 100)下提取植物的空中部分。采用UHPLC进行化学分析。分别用微量稀释试验、结晶紫、刚果红和eDNA测定来评估其抗菌活性、生物膜抑制作用、EPS和eDNA的产生。用MTT法评价了其对人角质形成细胞的细胞毒性和光保护作用。化学分析鉴定出64种化合物,包括苯甲酸和肉桂酸衍生物、类黄酮苷和柴胡皂苷。提取物对铜绿假单胞菌和耐甲氧西林金黄色葡萄球菌具有较强的抑菌活性(MIC低至0.5 mg/mL)。M提取物表现出适度的生物膜抑制和减少eDNA的产生。细胞毒性试验证实了对角质形成细胞的安全性,而M和us100提取物显示出光保护作用。镰刀草提取物在解决生物膜相关感染、抗生素耐药性和紫外线诱导的皮肤损伤方面显示出良好的潜力。另见图解摘要(图1)。1).
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling the antimicrobial, biofilm inhibition, and photoprotective potential of Bupleurum falcatum L. for dermatological applications.

Unveiling the antimicrobial, biofilm inhibition, and photoprotective potential of Bupleurum falcatum L. for dermatological applications.

Unveiling the antimicrobial, biofilm inhibition, and photoprotective potential of Bupleurum falcatum L. for dermatological applications.

Unveiling the antimicrobial, biofilm inhibition, and photoprotective potential of Bupleurum falcatum L. for dermatological applications.

Bupleurum falcatum L. is known for its therapeutic properties, especially in treating fever, inflammation, and infectious diseases. However, its potential for dermatological applications remains mainly unexplored. Thus, the present study explores antimicrobial potential of B. falcatum against biofilm-associated infections, antibiotic-resistant strains, and UV-induced skin damage. This aligns with the growing interest in natural products as sources of bioactive compounds with skin-protecting features. Herein, we employed maceration (M) and ultrasound-assisted extraction (USA) at 50 Hz and 100 Hz (USA 50 and USA 100) to obtain extracts from aerial parts of the plant. Chemical profiling was performed using UHPLC. Antimicrobial activity, biofilm inhibition, EPS and eDNA production were assessed using microdilution test, crystal violet, Congo red, and eDNA assays, respectively. Cytotoxicity and photoprotective effects were evaluated on human keratinocytes using the MTT assay. Chemical analysis identified 64 compounds, including benzoic and cinnamic acid derivatives, flavonoid glycosides, and saikosaponins. Extracts showed strong antimicrobial activity against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MIC as low as 0.5 mg/mL). The M extract displayed moderate biofilm inhibition and reduced eDNA production. Cytotoxicity assays confirmed safety on keratinocytes, while M and USA 100 extracts demonstrated photoprotective effects. B. falcatum extracts showed promising potential in addressing biofilm-associated infections, antibiotic resistance, and UV-induced skin damage. See also the graphical abstract(Fig. 1).

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来源期刊
EXCLI Journal
EXCLI Journal BIOLOGY-
CiteScore
8.00
自引率
2.20%
发文量
65
审稿时长
6-12 weeks
期刊介绍: EXCLI Journal publishes original research reports, authoritative reviews and case reports of experimental and clinical sciences. The journal is particularly keen to keep a broad view of science and technology, and therefore welcomes papers which bridge disciplines and may not suit the narrow specialism of other journals. Although the general emphasis is on biological sciences, studies from the following fields are explicitly encouraged (alphabetical order): aging research, behavioral sciences, biochemistry, cell biology, chemistry including analytical chemistry, clinical and preclinical studies, drug development, environmental health, ergonomics, forensic medicine, genetics, hepatology and gastroenterology, immunology, neurosciences, occupational medicine, oncology and cancer research, pharmacology, proteomics, psychiatric research, psychology, systems biology, toxicology
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