Xueqian Chu, Songmin Xue, Lanyan Lin, Xuan Huang, Sisi Chen, Wulaer Adeli, Zulibiya Tuohetiyaer, Suhua Li, Chen Lu
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Expression characteristics of dual-specificity phosphatase 2 and hypoxia-inducible factor-1α in acute kidney injury and preliminary study of the effect of dual-specificity phosphatase 2 on HK-2 cells.
Acute kidney injury (AKI) is a global health problem with significant long-term harm if the prognosis is poor. Dual-specificity phosphatase 2 (DUSP2) is involved in key regulatory pathways in several disease processes, but its function in renal pathophysiology is unclear. The expression levels of DUSP2 in the peripheral blood of AKI and non-AKI patients were first examined. The expression characteristics of DUSP2 and hypoxia-inducible factor-1α (HIF-1α) in AKI conditions were analysed in renal tissues from AKI patients and different AKI model mice. Human renal tubular epithelial cells (HK-2) were utilized for in vitro experiments to investigate the effects of overexpression of DUSP2 on cell proliferation, apoptosis and HIF-1α levels under hypoxia-reoxygenation conditions. DUSP2 showed low expression in the peripheral blood serum of AKI patients. In the renal tissues of patients and mouse models of AKI, DUSP2 expression was significantly lower, and HIF-1α expression was significantly higher. Under hypoxia-reoxygenation conditions, DUSP2 overexpression promoted HK-2 cell proliferation, inhibited apoptosis and suppressed HIF-1α expression. The present study demonstrated a significant correlation between DUSP2 and HIF-1α expression in the context of AKI and revealed the protective effect of DUSP2 overexpression on renal tubular epithelial cells under hypoxia-reoxygenation, which provides a new better understanding of AKI.
期刊介绍:
Experimental Physiology publishes research papers that report novel insights into homeostatic and adaptive responses in health, as well as those that further our understanding of pathophysiological mechanisms in disease. We encourage papers that embrace the journal’s orientation of translation and integration, including studies of the adaptive responses to exercise, acute and chronic environmental stressors, growth and aging, and diseases where integrative homeostatic mechanisms play a key role in the response to and evolution of the disease process. Examples of such diseases include hypertension, heart failure, hypoxic lung disease, endocrine and neurological disorders. We are also keen to publish research that has a translational aspect or clinical application. Comparative physiology work that can be applied to aid the understanding human physiology is also encouraged.
Manuscripts that report the use of bioinformatic, genomic, molecular, proteomic and cellular techniques to provide novel insights into integrative physiological and pathophysiological mechanisms are welcomed.