利用深度学习在不同疾病模型的微ct上自动量化肺病理。

IF 10.8 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
EBioMedicine Pub Date : 2025-09-01 Epub Date: 2025-08-30 DOI:10.1016/j.ebiom.2025.105904
Flore Belmans, Laura Seldeslachts, Eliane Vanhoffelen, Birger Tielemans, Wim Vos, Frederik Maes, Greetje Vande Velde
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引用次数: 0

摘要

背景:Micro-CT显著提高了呼吸系统疾病动物研究到人类临床试验的效率、预测能力和可转译性。然而,对大型微型ct数据集的分析仍然是一个瓶颈。方法:我们开发了一个通用的基于深度学习(DL)的肺分割模型,使用纵向微ct图像研究唐氏综合征,病毒和真菌感染,以及不同肺部病理和疾病负担程度的恶化。二维模型在轴、冠状和矢状切片上进行交叉验证。这些单一方向模型的预测结合起来,使用多数投票或概率平均来创建一个2.5D模型。测试了这些模型在其他研究(COVID-19、肺部炎症和纤维化)、扫描仪配置和啮齿动物物种(大鼠、仓鼠、鼠)中的通用性,包括一个公开可用的数据库。结果:在内部验证数据上,2.5D概率平均模型的Dice Similarity Coefficient (DSC)均值最高(0.953±0.023),通过去除肺区以外的错误体素,进一步提高了2D模型的输出。该模型具有良好的通用性,在不同的肺部病理和扫描仪配置下,平均DSC值为0.89至0.94。从手动和自动分割中提取的生物标志物非常一致,并证明我们提出的解决方案有效地监测纵向肺病理发展和对现实世界临床前研究中治疗的反应。解释:我们基于dl的肺病理定量流水线提供了大型微ct数据集的高效分析,广泛适用于啮齿动物疾病模型和采集方案,并能够实时洞察治疗效果。资金:本研究由瓦隆公共服务(AEROVID资助FB, WV)和佛兰德研究基金会(FWO,博士授权1SF2224N给EV和1186121N/1186123N给LS,基础设施资助I006524N给GVV)支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Automated quantification of lung pathology on micro-CT in diverse disease models using deep learning.

Background: Micro-CT significantly enhances the efficiency, predictive power and translatability of animal studies to human clinical trials for respiratory diseases. However, the analysis of large micro-CT datasets remains a bottleneck.

Methods: We developed a generic deep learning (DL)-based lung segmentation model using longitudinal micro-CT images from studies of Down syndrome, viral and fungal infections, and exacerbation with variable lung pathology and degree of disease burden. 2D models were trained with cross-validation on axial, coronal and sagittal slices. Predictions from these single-orientation models were combined to create a 2.5D model using majority voting or probability averaging. The generalisability of these models to other studies (COVID-19, lung inflammation and fibrosis), scanner configurations and rodent species (rats, hamsters, degus) was tested, including a publicly available database.

Findings: On the internal validation data, the highest mean Dice Similarity Coefficient (DSC) was found for the 2.5D probability averaging model (0.953 ± 0.023), further improving the output of the 2D models by removing erroneous voxels outside the lung region. The models demonstrated good generalisability with average DSC values ranging from 0.89 to 0.94 across different lung pathologies and scanner configurations. The biomarkers extracted from manual and automated segmentations are well in agreement and proved that our proposed solution effectively monitors longitudinal lung pathology development and response to treatment in real-world preclinical studies.

Interpretation: Our DL-based pipeline for lung pathology quantification offers efficient analysis of large micro-CT datasets, is widely applicable across rodent disease models and acquisition protocols and enables real-time insights into therapy efficacy.

Funding: This research was supported by the Service Public de Wallonie (AEROVID grant to FB, WV) and The Flemish Research Foundation (FWO, doctoral mandate 1SF2224N to EV and 1186121N/1186123N to LS, infrastructure grant I006524N to GVV).

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来源期刊
EBioMedicine
EBioMedicine Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
CiteScore
17.70
自引率
0.90%
发文量
579
审稿时长
5 weeks
期刊介绍: eBioMedicine is a comprehensive biomedical research journal that covers a wide range of studies that are relevant to human health. Our focus is on original research that explores the fundamental factors influencing human health and disease, including the discovery of new therapeutic targets and treatments, the identification of biomarkers and diagnostic tools, and the investigation and modification of disease pathways and mechanisms. We welcome studies from any biomedical discipline that contribute to our understanding of disease and aim to improve human health.
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